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Store samples and perform agglutination assays with Thermo Scientific™ Nunc™ 96 DeepWell™ Polystyrene Plates, featuring transparent round bottom deep wells that hold up to 900µL per well.

• • Alphanumeric grid provides quick and easy sample identification.

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• • Optimized for robotics and liquid handling systems

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• • Round-bottom wells for optimal sample recovery

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• • Untreated surface

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• • Sealable with well caps and sealing tape

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• • Customized bar coding is available

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This reducing agent-compatible (RAC) version of the Thermo Scientific™ Pierce BCA Protein Assay provides accurate determination of protein concentration in samples that contain dithiothreitol (DTT), 2-mercaptoethanol (BME), TCEP and other disulfide reducing agents.

The BCA Protein Assay Kit – Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA Assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, 2-ME or TCEP. Available in tube and microplate formats, these reducing agent compatible (RAC) BCA Kits extend the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.

Highlights:

• Compatible – assay samples that contain up to 5mM DTT, 35mM BME, or 10mM TCEP
• BCA technology – only a slight modification of the standard BCA Protein Assay protocol (15-minute incubation with Compatibility Reagent); no precipitation steps required
• Small samples – requires only 25μL (standard kit) or less than 10μL (microplate kit) of sample
• Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)
• Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods
• High linearity – linear working range for BSA equals 125 to 2000μg/mL

Includes:

BCA Reagents A and B, Compatibility Reagent, Reconstitution Buffer, and Albumin Standard (2mg/mL); Microplate kit also includes assay-compatible 96-well microplates

ActivX™ Desthiobiotin-GTP Probes contain a modified biotin attached to the nucleotide by a labile acyl-phosphate bond. ActivX active-site enrichment probes are advantageous for determining active enzyme levels through the use of analog-tagged probes to allow profiling of both inactive and active enzymes. The ActivX probes selectively enrich only those enzymes that are functionally active and biologically relevant at the time of labeling. With selective enrichment, one can identify and profile target enzyme classes across samples or can assess the specificity and affinity of enzyme inhibitors.

After removal of GTP or GDP nucleotides from enzymes, the desthiobiotin-GTP probe can be used to covalently modify conserved lysine residues in the GTPase nucleotide-binding site. Desthiobiotin-GTP can selectively enrich, identify and profile target enzyme classes in samples. Pre-incubation of samples with small-molecule inhibitors that compete with active-site probes can be used to determine inhibitor binding affinity and target specificity. The ActivX Desthiobiotin-GTP Probe covalently labels the active site of GTPases and GTPase subunits of G-protein coupled receptors.

Applications of ActivX Desthiobiotin-GTP Probe:
• Broad enrichment of GTP-binding proteins from tissues, cells and sub-cellular proteomes
• Enrichment of enzymes based on function
• Profiling of dozens to hundreds of inhibitor targets

Assessment of active-site labeling with desthiobioin probes can be accomplished by either Western blot or mass spectrometry (MS). For the Western blot workflow, desthiobiotin-labeled proteins are enriched for SDS-PAGE analysis and subsequent detection with specific antibodies. For the MS workflow, desthiobiotin-labeled proteins are reduced, alkylated and enzymatically digested to peptides. Only the desthiobiotin-labeled, active-site peptides are enriched for analysis by LC-MS/MS. Both workflows can be used for determining inhibitor target binding, but only the MS workflow can identify global inhibitor targets and off-targets.

These products are subject to a limited use label license.

Thermo Scientific™ Pierce Agaroses are highly purified and carefully blended formulations of regular- or low-melting agarose that provide uniform lot-to-lot consistency for preparation of electrophoresis gels.

Highlights:

• Agarose I (Molecular Biology Grade)
• A regular melting-temperature agarose for electrophoresis requiring an exceptional level of purity with unequaled performance
• Agarose II (Low Melt)
• A low temperature agarose for special applications requiring a lower melting temperature
• Optimal for “in-gel” restriction digestion/ligation and DNA recovery
• At 1%, forms a very manageable clear gel that provides excellent resolution and melting characteristics
• Melts at approximately 65°C and remains fluid at 37°C for at least 24 hours, providing an excellent matrix for enzymatic manipulation of samples

Thermo Scientific™ Pierce Agaroses are highly purified and carefully blended formulations of regular- or low-melting agarose that provide uniform lot-to-lot consistency for preparation of electrophoresis gels.

Highlights:

• Agarose I (Molecular Biology Grade)
• A regular melting-temperature agarose for electrophoresis requiring an exceptional level of purity with unequaled performance
• Agarose II (Low Melt)
• A low temperature agarose for special applications requiring a lower melting temperature
• Optimal for “in-gel” restriction digestion/ligation and DNA recovery
• At 1%, forms a very manageable clear gel that provides excellent resolution and melting characteristics
• Melts at approximately 65°C and remains fluid at 37°C for at least 24 hours, providing an excellent matrix for enzymatic manipulation of samples

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Thermo Scientific™ Protease Inhibitors are small packages of individual protease inhibitor peptides and compounds for customized formulation or modification of protease inhibitor cocktails.

The individual reagents include AEBSF, aprotinin, bestatin, E64, leupeptin, pepstatin A, and PMSF. Although Thermo Scientific Halt™ Protease Inhibitor Cocktails provide convenient and optimized broad-spectrum protease inhibition for routine cell lysis and assay needs, certain applications require a more customized approach. By offering the individual high-quality reagents used to make our cocktail products, we provide you with the tools needed to apply a protease inhibitor individually, supplement a cocktail, or combine several components to make customized protease inhibitor cocktails for specialized applications.

Highlights:

• High-quality reagents in convenient and affordable package sizes
• Purchase individually to prepare customized protease inhibitor cocktails
• Supplement Halt Protease Inhibitor Single-Use Cocktails, whose formulations are fully disclosed, to achieve desired concentrations of specific component reagents

Perform quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ Black 96-Well Immuno Plates. Featuring a choice of three surface types and optimal binding, these plates minimize background in fluorescence reading.   Features:

• • Plate has SBS footprint — fits standard equipment

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• • Available with flat- or round-bottom wells

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• • Certified binding homogeneity/reproducibility

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nChoice of surface:n

• • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) are for absorption of hydrophobic molecules.

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• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays.

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• • Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules.

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nCompatible with:nnCompatible with lids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Perform quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ Black 96-Well Immuno Plates. Featuring a choice of three surface types and optimal binding, these plates minimize background in fluorescence reading.

Features:

• • Plate has SBS footprint — fits standard equipment

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• • Available with flat- or round-bottom wells

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• • Certified binding homogeneity/reproducibility

n

nChoice of surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) are for absorption of hydrophobic molecules.

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays.

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• • Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules.

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Compatible with: Compatible with lids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Perform quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ Black 96-Well Immuno Plates. Featuring a choice of three surface types and optimal binding, these plates minimize background in fluorescence reading.Features:

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Certified binding homogeneity/reproducibility

n

nChoice of surface:n

• • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) are for absorption of hydrophobic molecules.

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays.

n

• • Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules.

n

Compatible with: Compatible with lids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Perform quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ Black 96-Well Immuno Plates. Featuring a choice of three surface types and optimal binding, these plates minimize background in fluorescence reading.Features:

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Certified binding homogeneity/reproducibility

n

nChoice of surface:n

• • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) are for absorption of hydrophobic molecules.

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays.

n

• • Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules.

n

Compatible with: Compatible with lids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Thermo Scientific™ Blocker BSA Blocking Buffers are ready-to-use, 10X PBS or TBS solutions of bovine serum albumin protein for blocking steps in Western blotting, ELISA, immunohistochemistry and nucleic acid detection methods.

nThese blocking buffers are 10% (w/v) solutions of high-quality BSA that are useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. Blocker BSA Buffers are most frequently diluted 10-fold (to 1% BSA) for initial testing, but other buffer concentrations can be beneficial for specific systems. Blocker BSA is usually more effective than nonfat milk blocking buffers for biotin-avidin systems because it contains a single purified protein that is devoid endogenous biotin.nnHighlights:n

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  • Purified protein – 10% solutions of high-quality bovine serum albumin; a single purified protein provides fewer chances of cross-reaction with assay components than serum or milk solutions

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• • Convenient – concentrated formulation saves storage space and can be diluted easily to obtain optimal blocking results for specific applications

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• • Easy to use – no waiting for powder to dissolve with this ready-to-dilute liquid concentrate

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• • Flexible – available in PBS and TBS formulations to suit a variety of applications

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Thermo Scientific™ Bond-Breaker TCEP Solution, Neutral pH is a stable, 0.5M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulfide bonds.

nHighlights:n

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  • Ready-to-use, odorless, stable and neutral 0.5M TCEP solution

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• • Eliminates TCEP·HCl stock solution preparation and neutralization prior to SDS-PAGE gel loading

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• • Convenient, time-saving format for TCEP reductions

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• • Neutral pH minimizes possibility of amide bond cleavage during reduction

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• • Room temperature-stable, saves valuable refrigerator space

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• • Contributes to more pleasant, safer laboratory environment

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• • Unequalled high-resolution separations

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• • Exceptionally high efficiency

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• • Highest resolution of monoclonal antibody variants

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• • Unmatched column-to-column and lot-to-lot reproducibility

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• • Hydrophobic interactions eliminated

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• • Excellent for stability testing

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• • Exceptionally high capacity provides exceptionally high yields with high purity

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• • Refined selectivity for high resolution of laboratory-scale oligonucleotide purification by anion-exchange chromatography

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• • Compatible with high pH, solvents, and high temperatures

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• • Pressure and chemically stable monolith support coated with functionalized latex nanobeads

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Thermo Scientific™ Easy-Titer Antibody Assay Kits enable accurate determination of antibody concentration in serum and culture supernatant, and include kits specific for mouse IgG, rabbit IgG, human IgG (H+L), human IgG (Fc), or human IgM.

These kits include antibody-sensitized microspheres to measure the specific concentration of mouse, rabbit and human antibodies by an easy and rapid micro-agglutination technique using standard microplates and UV-Vis plate reader (spectrophotometer). Each kit is specific for a particular species and class of immunoglobulin and, unlike total protein assays, can specifically measure the concentration of target antibody in samples (e.g., serum, plasma, culture supernatant) that contain other proteins. The kits are extremely sensitive, requiring very small sample volumes. Antibody concentration is determined from the assay response (absorbance) by comparison to a standard curve prepared using dilutions of a known antibody sample (sold separately).

Highlights:

• Kits for five popular targets – choose a kit specific for a particular species and class of immunoglobulin; no cross-reaction with other species and classes of the target antibody
• Antibody-based specificity – measure concentration of target antibody in a sample, not just total protein; no need to purify antibody to assess its concentration
• Faster and easier than ELISA – three-component, homogenous assay; 10 minutes total incubation time
• No special equipment needed – uses standard vortex mixer, pipetter, 96-well microplate, plate shaker and reader (measure absorbance at 340nm or 400nm)
• Sensitive – assay range (standard curve) 8 to 500ng/mL; requires starting sample of at least 300ng/mL
• Reproducible – coefficient of variation less than 5%; error depends on dilution and pipetting technique
• Antibody standards sold separately – use any antibody standard with proper target identity and known concentration (greater than 10μg/mL)

Easy-Titer Antibody Assays are faster and easier than ELISA:

1. Prepare standards (5 to 500ng/mL) by diluting purified antibody in Kit Dilution Buffer

2. Prepare samples by diluting at least 20-fold in Dilution Buffer to within assay range (8 to 500ng/mL).

3. Vortex vial of microsphere beads to create homogeneous suspension.

4. Pipette 20μL of bead suspension and 20μL of each sample and standard into 96-well microplate wells.

5. Incubate microplate for 5 minutes with vigorous mixing.

6. Add 100μL of Kit Blocking Reagent.

7. Incubate microplate for 5 minutes with vigorous mixing.

8. Measure absorbance on standard plate reader (340nm or 405nm).

9. Plot standard curve and interpolate samples to determine concentration.

Includes:

Each kit contains target-specific IgG sensitized microspheres, dilution buffer and blocking buffer

Thermo Scientific™ Easy-Titer Antibody Assay Kits enable accurate determination of antibody concentration in serum and culture supernatant, and include kits specific for mouse IgG, rabbit IgG, human IgG (H+L), human IgG (Fc), or human IgM.

These kits include antibody-sensitized microspheres to measure the specific concentration of mouse, rabbit and human antibodies by an easy and rapid micro-agglutination technique using standard microplates and UV-Vis plate reader (spectrophotometer). Each kit is specific for a particular species and class of immunoglobulin and, unlike total protein assays, can specifically measure the concentration of target antibody in samples (e.g., serum, plasma, culture supernatant) that contain other proteins. The kits are extremely sensitive, requiring very small sample volumes. Antibody concentration is determined from the assay response (absorbance) by comparison to a standard curve prepared using dilutions of a known antibody sample (sold separately).

Highlights:

• Kits for five popular targets – choose a kit specific for a particular species and class of immunoglobulin; no cross-reaction with other species and classes of the target antibody
• Antibody-based specificity – measure concentration of target antibody in a sample, not just total protein; no need to purify antibody to assess its concentration
• Faster and easier than ELISA – three-component, homogenous assay; 10 minutes total incubation time
• No special equipment needed – uses standard vortex mixer, pipetter, 96-well microplate, plate shaker and reader (measure absorbance at 340nm or 400nm)
• Sensitive – assay range (standard curve) 8 to 500ng/mL; requires starting sample of at least 300ng/mL
• Reproducible – coefficient of variation less than 5%; error depends on dilution and pipetting technique
• Antibody standards sold separately – use any antibody standard with proper target identity and known concentration (greater than 10μg/mL)

Easy-Titer Antibody Assays are faster and easier than ELISA:

1. Prepare standards (5 to 500ng/mL) by diluting purified antibody in Kit Dilution Buffer

2. Prepare samples by diluting at least 20-fold in Dilution Buffer to within assay range (8 to 500ng/mL).

3. Vortex vial of microsphere beads to create homogeneous suspension.

4. Pipette 20μL of bead suspension and 20μL of each sample and standard into 96-well microplate wells.

5. Incubate microplate for 5 minutes with vigorous mixing.

6. Add 100μL of Kit Blocking Reagent.

7. Incubate microplate for 5 minutes with vigorous mixing.

8. Measure absorbance on standard plate reader (340nm or 405nm).

9. Plot standard curve and interpolate samples to determine concentration.

Includes:

Each kit contains target-specific IgG sensitized microspheres, dilution buffer and blocking buffer

Thermo Scientific™ Easy-Titer Antibody Assay Kits enable accurate determination of antibody concentration in serum and culture supernatant, and include kits specific for mouse IgG, rabbit IgG, human IgG (H+L), human IgG (Fc), or human IgM.

These kits include antibody-sensitized microspheres to measure the specific concentration of mouse, rabbit and human antibodies by an easy and rapid micro-agglutination technique using standard microplates and UV-Vis plate reader (spectrophotometer). Each kit is specific for a particular species and class of immunoglobulin and, unlike total protein assays, can specifically measure the concentration of target antibody in samples (e.g., serum, plasma, culture supernatant) that contain other proteins. The kits are extremely sensitive, requiring very small sample volumes. Antibody concentration is determined from the assay response (absorbance) by comparison to a standard curve prepared using dilutions of a known antibody sample (sold separately).

Highlights:

• Kits for five popular targets – choose a kit specific for a particular species and class of immunoglobulin; no cross-reaction with other species and classes of the target antibody
• Antibody-based specificity – measure concentration of target antibody in a sample, not just total protein; no need to purify antibody to assess its concentration
• Faster and easier than ELISA – three-component, homogenous assay; 10 minutes total incubation time
• No special equipment needed – uses standard vortex mixer, pipetter, 96-well microplate, plate shaker and reader (measure absorbance at 340nm or 400nm)
• Sensitive – assay range (standard curve) 8 to 500ng/mL; requires starting sample of at least 300ng/mL
• Reproducible – coefficient of variation less than 5%; error depends on dilution and pipetting technique
• Antibody standards sold separately – use any antibody standard with proper target identity and known concentration (greater than 10μg/mL)

Easy-Titer Antibody Assays are faster and easier than ELISA:

1. Prepare standards (5 to 500ng/mL) by diluting purified antibody in Kit Dilution Buffer

2. Prepare samples by diluting at least 20-fold in Dilution Buffer to within assay range (8 to 500ng/mL).

3. Vortex vial of microsphere beads to create homogeneous suspension.

4. Pipette 20μL of bead suspension and 20μL of each sample and standard into 96-well microplate wells.

5. Incubate microplate for 5 minutes with vigorous mixing.

6. Add 100μL of Kit Blocking Reagent.

7. Incubate microplate for 5 minutes with vigorous mixing.

8. Measure absorbance on standard plate reader (340nm or 405nm).

9. Plot standard curve and interpolate samples to determine concentration.

Includes:

Each kit contains target-specific IgG sensitized microspheres, dilution buffer and blocking buffer

Use Thermo Scientific™ Electronic Hand-Held Vial Crimpers and Decrimpers to attach or remove crimp caps from vials, respectively. These hand-held, high-power crimpers and decrimpers are available in 8 mm, 11 mm, 13 mm, and 20 mm sizes and allow you to seal or remove crimp caps with a simple, one-handed push of a button. Use for crimping of vials with aluminum, pressure-release, and bi-metal caps.nnThe electronic crimper’s message window conveniently provides all-important information at one glance. This device is Performance Level 3, which means it is designed to protect your most valuable samples and deliver consistent, reproducible results for your most demanding analyses.

Thermo Scientific™ Pierce EMCS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 Ås).

Highlights:

• Reactive groups: NHS ester and maleimide
• Reactive toward: amino and sulfhydryl groups

Thermo Scientific™ Pierce Ethidium Bromide is available as both dilute (0.625mg/mL) and more concentrated (10mg/mL) solutions to accommodate most molecular biology applications for fluorescent DNA visualization.

Thermo Scientific™ Pierce Ethidium Bromide is available as both dilute (0.625mg/mL) and more concentrated (10mg/mL) solutions to accommodate most molecular biology applications for fluorescent DNA visualization.

Highlights:

0.625mg/mL EtBr Solution:

• Each drop contains 25μg of ethidium bromide and is ideal for a 50mL agarose gel
• After adding one drop of solution per 50mL of gel, the final concentration of EtBr will be 0.5μg/mL, which is the recommended concentration for electrophoresis of nucleic acids
• Can also be used as the running buffer or for staining gels after electrophoresis

10mg/mL EtBr Solution:

• A safe and convenient way to use ethidium bromide while minimizing exposure

Thermo Scientific™ Pierce Fluoraldehyde (OPA) is o-phthalaldehyde, a highly sensitive fluorescent pre- or post-column derivatization reagent for peptide or amino acid detection and quantitation in HPLC.

Fluoraldehyde Crystals are a specially purified, fluorogenic-grade preparation of o-phthalaldehyde (OPA). This amine-reactive compound is primarily used for high-sensitivity, fluorescent detection of primary amines, including amino acids, peptides, proteins and polyamines in pre- or post-column chromatographic effluents.

Highlights:

• For pre- or post-column amino acid derivatization for fluorescent detection and quantitation
• Reacts with all primary amine-containing analytes to yield fluorescent derivatives (ex/em = 340nm/455nm)
• Provides an accurate measure of both composition and absolute protein-peptide content
• Ideal for work with recombinant proteins and synthetic peptides
• Can be used for fluorescent protein or peptide assay
• Can be prepared as a stable aqueous solution in borate (see instructions)

Reagent Properties:

• Can be prepared as a stable aqueous solution in borate (see instructions)
• Alternative names: Fluoraldehyde Crystals, OPA
• Chemical name: ortho-Phthalaldehyde
• Reactive toward: Primary amines (–NH2)
• Chemical formula: C8H6O2
• CAS number: 643-79-8
• Molecular weight: 134.12
• Form: Pale yellow, free-flowing crystals

Thermo Scientific™ Pierce Fluoraldehyde (OPA) Reagent is a ready-to-use solution of o-phthalaldehyde, a fluorescent pre- or post-column derivatization reagent for peptide or amino acid detection and quantitation in HPLC.

Fluoraldehyde Reagent contains OPA (o-phthalaldehyde), which reacts with primary amines of amino acids, peptide and proteins to enable fluorescent detection and quantitation. The reagent that can be used as a protein or peptide assay reagent or as a pre- or post-column detection reagent for amino acid analysis (HPLC). Reaction of OPA with proteins and peptides yields linear results over a wide range of concentrations. The Fluoraldehyde Reagent Solution is supplied ready to use and enables fast quantitation of proteins or peptides in solution.

Highlights:

• For pre- or post-column amino acid derivatization for fluorescent detection and quantitation
• Reacts with all primary amine-containing analytes to yield fluorescent isoindole derivatives
• Provides an accurate measure of both composition and absolute protein-peptide content
• Ideal for work with recombinant proteins and synthetic peptides
• Can be used for fluorescent protein or peptide assay
• Pre-column derivatization mixtures can be injected into LC without any processing

Reagent Properties:

• Formulation: 0.8mg/mL o-phthalaldehyde (OPA) in proprietary buffer, pH 10
• Form: Clear, colorless to slightly yellow liquid
• Reactive toward: Primary amines (–NH2)
• Detection: Fluorescence (excitation/emission = 340nm/455nm)
• Application: Pre- or post-column derivatization for detection by HPLC

Thermo Scientific™ Pierce Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as the percent of total purified protein mass.

The Glycoprotein Carbohydrate Estimation Kit includes six purified glycoprotein standards and the required assay reagents to effectively estimate the amount of oxidizable glycosylation (% carbohydrate by weight) of purified protein samples. Sugar groups in the glycoprotein sample are first oxidized with sodium meta-periodate to produce detectable aldehyde groups. Then the sample is reacted with the colorimetric Glycoprotein Detection Reagent. Absorbance at 550nm of the resulting purple reaction product is measured with a spectrophotometer or plate reader. Finally, the carbohydrate content in the glycoprotein is calculated by comparison to results from the five glycoprotein standards that are included in the kit.

Highlights:

• Qualitative – easily identifies purified proteins as glycoproteins or samples as contaminated with sugars
• Semi-quantitative – estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards
• Simple procedure – completed in less than 75 minutes; instructions include microplate and test tube protocols
• Adaptable – standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components

Includes:

Sodium periodate, assay buffer, detection reagent and a 5-vial set of glycoprotein standards

Recommended for:

Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents; Characterize and classify purified protein fractions from an affinity procedure; Assess sugar and carbohydrate contamination in non-glycosylated protein samples

Thermo Scientific™ Pierce GMBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (7.3 Ås).

Highlights:

• Less immunogenic than SMCC
• Reactive groups: NHS ester and maleimide
• Reactive toward: amino and sulfhydryl groups

This broad-spectrum Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases. The 100X cocktails, available in regular and EDTA-free formulations and several package formats, are refrigerator-stable liquids that are very easy to use for nearly any sample or experimental scale. Highlights:

• • Complete protection all-in-one cocktail contains both protease and phosphatase inhibitors

n

• • Multiple package sizes choose from four convenient packages sizes to match nearly any scale

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• • Convenient the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats

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• • Easy storage aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails

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• • Compatible can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift

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• • Validated fully compatible with Thermo Scientific Pierce™ Cell Lysis Buffers

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nIncludes:nnFormulations with and without supplementary vial of EDTA for metalloprotease inhibition, both of which are offered in four package sizes.

This broad-spectrum Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases. The 100X cocktails, available in regular and EDTA-free formulations and several package formats, are refrigerator-stable liquids that are very easy to use for nearly any sample or experimental scale. Highlights:

• • Complete protection all-in-one cocktail contains both protease and phosphatase inhibitors

n

• • Multiple package sizes choose from four convenient packages sizes to match nearly any scale

n

• • Convenient the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats

n

• • Easy storage aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails

n

• • Compatible can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift

n

• • Validated fully compatible with Thermo Scientific Pierce™ Cell Lysis Buffers

n

nIncludes:nnFormulations with and without supplementary vial of EDTA for metalloprotease inhibition, both of which are offered in four package sizes.

Glucoside detergents are a class of non-ionic detergents commonly used in biochemical and biophysical research. These detergents are derived from glucose and possess unique properties that make them suitable for various applications. Glucoside detergents are known for their mildness, biocompatibility, and ability to solubilize and stabilize a wide range of biomolecules, including membrane proteins. Glucoside detergents are also compatible with many downstream applications, such as protein purification, crystallization, and structural studies.

Features of glucoside detergents
• Gentle on proteins, minimizing denaturation
• Non-ionic nature—reduces electrostatic interactions
• Low critical micelle concentration (CMC)
• High-purity with low UV absorptivity

Overall, glucoside detergents are versatile and can be used in various experimental techniques, including protein purification, crystallization, and structural biology studies. They are compatible with a wide range of biomolecules and can be employed in both membrane and soluble protein research. With their gentle nature and versatile performance, glucoside detergents have become valuable tools in the field of biochemical research.

Octyl-beta-Glucoside
Octyl-beta-Glucoside (OG) is a low molecular weight, non-ionic detergent with an octyl chain attached to the glucose moiety that has been widely used for membrane protein solubilization. OG is generally more soluble in aqueous solutions and forms clear and stable solutions at higher concentrations. OG typically forms larger micelles compared to OTG. The larger micelles of OG can be advantageous for certain applications, such as protein purification or structural studies, as they provide better solubility and reduced interference during downstream processes.

Properties of OG
• Alternative names: octyl-beta-glucopyranoside, octyl-beta-D-glucopyranoside
• Molecular weight: 292.37 g
• Micelle molecular weight: 8000 g
• Critical micelle concentration (CMC): 23 to 25 mM (0.6716 to 0.7300%, w/v)
• Aggregation number: 27
• Cloud point: >100°C
• Optically clear: low absorbance at 280 nm

Octylthio Glucoside
Octylthio Glucoside (OTG) is a low molecular weight, nonionic detergent that is effective for cell lysis and nondenaturing protein solubilization. It is resistant to beta-D-glucoside glucohydrolase degradation because the thioether group present in OTG provides protection against enzymatic hydrolysis. As a result, OTG can remain stable in the presence of beta-D-glucoside glucohydrolase enzymes, making it useful in applications where enzymatic stability is desired. However, it is important to note that the specific stability of OTG can still be influenced by factors such as enzyme concentration, reaction conditions, and the specific enzymes present.

Properties of OTG
• Chemical name: n-octyl-β-D-thioglucopyranoside
• Molecular weight: 44 g
• CMC: 9 mM (0.2772%, w/v)
• Cloud point: >100°C
• Optically clear: low absorbance at 280 nm

n-Nonyl-β-D-glucoside
n-Nonyl-β-D-glucoside (NG) has a longer alkyl chain (nonyl) compared to other glucosides. The longer alkyl chain in n-Nonyl-β-D-glucoside can confer higher hydrophobicity and potentially stronger detergent properties. This can enhance the solubilization of hydrophobic biomolecules and improve the extraction of membrane proteins. It may also induce different interactions with lipid bilayers, influencing the stability, fluidity, and permeability of the lipid bilayer, potentially impacting the behavior of membrane proteins. In addition, NG forms larger micelles compared to glucosides with shorter alkyl chains.

Properties of NG
• CMC: (H2O) ~ 6.5 mM (0.20%)
• Aggregation number: ~ 133
• Purity: ≥ 99% β+α (by HPLC analysis)
• pH: 5-8 (1% solution in water)

Thermo Scientific™ HisProbe-HRP is an nickel (Ni²+)-activated derivative of horseradish peroxidase (HRP). This product has been optimized for direct detection of recombinant histidine-tagged proteins and other histidine-rich proteins.

Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The detection kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC).

Highlights:

• Fast – His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol
• Economical – no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies
• High specificity – HisProbe-HRP can recognize his-tags independent of adjacent tags
• Less steric hindrance – HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies

Thermo Scientific™ Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of “heavy“ ¹³C- or ¹⁵N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

• Efficient – 100% label incorporation into proteins of living cells
• Flexible – different isotopes of heavy and/or light amino acids for arginine, lysine, leucine and proline enable the quantitation of peptides derived from MS-grade proteases
• Multiplex capabilities – several alternative isotopes of arginine and lysine are available that allow the analysis of multiple treatment conditions in each experiment
• High-quality supplements – heavy amino acids with >99% isotope purity

Get a gas-tight syringe for manual injections in your GC system. This syringe features a Luer-Tip connection and is available in a variety of sizes. Made of quality materials, they are designed for easy, accurate, precise and reproducible injections.

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter.

Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

Get a gas-tight syringe for manual injections in your GC system. This syringe features a Luer-Tip connection and is available in a variety of sizes. Made of quality materials, they are designed for easy, accurate, precise and reproducible injections.

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter.

Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

Thermo Scientific™ Pierce MBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short aromatic spacer arm (7.3 Ås).

Highlights:

• Popular for forming enzyme immunoconjugates
• Reactive groups: NHS-ester and maleimide
• Reactive toward: amino and sulfhydryl groups

Perform many small-volume applications with these safe diagnostic bottles that are leakproof when used with Nalgene closures. Ideal for use in test kits, sample storage and reagent shipping.

Perform many small-volume applications with Thermo Scientific™ Nalgene™ HDPE Diagnostic Bottles with Closure. These bottles are ideal for use in test kits, sample storage and shipping of small-volume, valuable reagents. They come packed in rigid trays for easy handling.

Save time and minimize contamination with gamma-sterilized Thermo Scientific™ Nalgene™ Rapid-Flow™ Filter Storage Bottles. Use with Nalgene Bottle-Top filters or as replacement/extra receptacles for filter units. Excellent as single-use storage containers.

• • Headspace allows for extra serum or other additives to be added to the filtrate

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• • Leakproof† screw caps to eliminate pH shift in bottle for longer life of stored media

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• • Sterilized by gamma radiation and individually bagged for a 5-year sterile shelf life

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• • Non-pyrogenic and non-cytotoxic

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• • Available with Fit for Purpose Sterile documentation

Increase filtration efficiency. Thermo Scientific™ Nalgene™ Rapid-Flow™ single use bottle tops feature the exclusive Rapid-Flow support column design, providing fast flow rates and high throughput. The last line of defense against cell culture contamination—Nalgene Rapid-Flow bottle tops are the best choice for the filtration of cell culture media, serum, additives and buffers.

• • Available in PES and SFCA membranes. Devices are color coded according to membrane.

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• • Devices feature the fastest flow rate and lowest clogging of comparable alternatives

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• • 0.1um PES available for mycoplasma protection

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• • 0.2um PES devices are stem cell and mouse embryonic assay (MEA) tested

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• • Non-pyrogenic and endotoxin free – Rapid-Flow filters meet the most rigorous testing standards for any vacuum filtration unit

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• • Available with Fit for Purpose Sterile documentation

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This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Prevent chemical accidents in the lab with these FEP Wash Bottles. Bottles labeled following the Globally Harmonized System (GHS).

Prevent chemical accidents in the lab with Nalgene™ Right-to-Understand FEP Wash Bottles. Bottles are permanently etched with chemical identification and safe handling information following the Globally Harmonized System (GHS) specifications as applicable to secondary laboratory containers. Wash bottles are ready to use when you transfer harsh laboratory chemicals from manufacturers’ containers. Six bottles available. For use with methyl ethyl ketone; n-hexane; toluene; xylene; methylene chloride; acetone.

Temporarily contain waste on the benchtop or under the lab hood using Thermo Scientific™ Nalgene™ Safety Waste Funnel Systems. Funnels feature the Easy Snap Safety Latch to minimize spillage if accidentally tipped over and a larger diameter funnel for easy pouring. The vent system helps reduce fumes and splashing while the removable neck screen traps large particles, like stir bars. Designed for solvent, chemical and biological waste, it is recommended for use with Nalgene containers.

Features:

• Polypropylene closure and funnel adaptor, polytetrafluoroethylene vent membrane
• Available in 10″ diameter funnel with 83B closure and 5.5″ funnel with 38-430 closure

Includes:

• HDPE funnel with attached lid
• Polypropylene closure/funnel adapter
• Screen insert
• Polypropylene/PTFE 0.45μm vent plug
• Filter

Thermo Scientific™ Nunc 15mL & 50mL Conical Sterile Polypropylene Centrifuge Tubes are premium, high-quality conical tubes that are environmentally friendly, offer the highest cleanliness with a recyclable plastic rack and allow for increased traceability with the largest writing area on the market. Nunc Labware Products are made from high-purity resins and molded using our state-of-the-art processes. Plastic labware is a safer alternative to glass without sacrificing accuracy.

• • Environmentally friendly recyclable plastic rack that reduces contamination from traditional foam packaging

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• • Leakproof to protect your samples

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Thermo Scientific™ Nunc 15mL & 50mL Conical Sterile Polypropylene Centrifuge Tubes are premium, high-quality conical tubes that are environmentally friendly, offer the highest cleanliness with a recyclable plastic rack and allow for increased traceability with the largest writing area on the market. Nunc Labware Products are made from high-purity resins and molded using our state-of-the-art processes. Plastic labware is a safer alternative to glass without sacrificing accuracy.

• • Environmentally friendly recyclable plastic rack that reduces contamination from traditional foam packaging

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• • Leakproof to protect your samples

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Thermo Scientific™ Nunc 15mL & 50mL Conical Sterile Polypropylene Centrifuge Tubes are premium, high-quality conical tubes that are environmentally friendly, offer the highest cleanliness with a recyclable plastic rack and allow for increased traceability with the largest writing area on the market. Nunc Labware Products are made from high-purity resins and molded using our state-of-the-art processes. Plastic labware is a safer alternative to glass without sacrificing accuracy.

• • Environmentally friendly recyclable plastic rack that reduces contamination from traditional foam packaging

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• • Leakproof to protect your samples

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Thermo Scientific™ Nunc 15mL & 50mL Conical Sterile Polypropylene Centrifuge Tubes are premium, high-quality conical tubes that are environmentally friendly, offer the highest cleanliness with a recyclable plastic rack and allow for increased traceability with the largest writing area on the market. Nunc Labware Products are made from high-purity resins and molded using our state-of-the-art processes. Plastic labware is a safer alternative to glass without sacrificing accuracy.

• • Environmentally friendly recyclable plastic rack that reduces contamination from traditional foam packaging

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• • Leakproof to protect your samples

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Store samples and perform agglutination assays with Thermo Scientific™ Nunc™ 96 DeepWell™ Polystyrene Plates, featuring transparent round bottom deep wells that hold up to 900µL per well.

• • Alphanumeric grid provides quick and easy sample identification.

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• • Optimized for robotics and liquid handling systems

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• • Round-bottom wells for optimal sample recovery

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• • Untreated surface

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• • Sealable with well caps and sealing tape

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• • Customized bar coding is available

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Store samples and perform agglutination assays with Thermo Scientific™ Nunc™ 96 DeepWell™ Polystyrene Plates, featuring transparent round bottom deep wells that hold up to 900µL per well.

• • Alphanumeric grid provides quick and easy sample identification.

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• • Optimized for robotics and liquid handling systems

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• • Round-bottom wells for optimal sample recovery

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• • Untreated surface

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• • Sealable with well caps and sealing tape

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• • Customized bar coding is available

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Increase storage capacity and improve mixing using Thermo Scientific™ Nunc™ 1.3 and 2.0mL DeepWell™ Plates with Shared-Wall Technology. Ideal for sample collection, storage, combinatorial chemistry and library applications, and resistant to most chemicals, solvents and alcohols used in combinatorial chemistry.

Features:

• • Ideal as a collection plate for Nunc Filter Plates

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• • Storage plate for compounds, samples or biomolecules

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• • Round-bottom wells reduce liquid retention

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• • Natural color

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• • Customized barcoding is available

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Working volumes:

• • 260251 and 260252 50-1000µL/well

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• • 278743 and 278752 50-1900 µL/well

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Recommended for: Sample collection; storage; combinatorial chemistry; library applications; bacterial and yeast growth; collection plates for Nunc filter plates

Perform adherent cell cultures in biotechnology, pharmaceutical and research laboratories, as well as in the production of vaccines and diagnostic kits with Thermo Scientific™ Nunc™ Cell Culture Tubes.

Features:

• Polystyrene tubes, polyethylene caps
• Flat-sided tube provides 5.5cm² culture area for grow of adherent cells
• Flat-sided tube allows easy microscopy; a coverslip can be used in the tube
• Medium retained in the flat-sided tube in horizontal position
• Treated with Thermo Scientific™ Nunclon™ Delta covering

Perform adherent cell cultures in biotechnology, pharmaceutical and research laboratories, as well as in the production of vaccines and diagnostic kits with Thermo Scientific™ Nunc™ Cell Culture Tubes.

Features:

• Polystyrene tubes, polyethylene caps
• Flat-sided tube provides 5.5cm² culture area for grow of adherent cells
• Flat-sided tube allows easy microscopy; a coverslip can be used in the tube
• Medium retained in the flat-sided tube in horizontal position
• Treated with Thermo Scientific™ Nunclon™ Delta covering

Non-treated Thermo Scientific™ Nunc™ Rectangular Dishes with ANSI™ standard dimensions are optimized for molecular biology applications using robotic instruments.

Rectangular Dishes 1, 4 and 8-Well

• • 1-well dish is ideal for HTS Petri dish applications

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• • 4- and 8-well dishes are ideal for assay compartmentalization, probe multiple gene sub-set

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• • 4-well dish holds four glass slides for storage

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n1-Well Format

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  • Convenient plate format for cell culture

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• • HTS Petri dish applications

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n4- and 8-Well Formatsn

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  • Assay compartmentalization

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• • Probe multiple gene subsets

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• • Wash Western blot strips

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• • 4-well dish holds four glass slides

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• • Useful in all areas of cell culture, including scale-up and cloning

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Ordering Information: Customized bar coding is available