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Tube contains 0.5 mm diameter yttria-stabilized zirconium oxide beads. Used to lyse yeast, fungi, algae. For use on FastPrep® instruments or any bead beater.

Tube contains 0.5 mm diameter yttria-stabilized zirconium oxide beads. Used to lyse yeast, fungi, algae. For use on FastPrep® instruments or any bead beater.

Tube contains 0.5 mm diameter yttria-stabilized zirconium oxide beads. Used to grind soft animal and plant tissues. For use on FastPrep® instruments or any bead beater.

Tube contains 0.5 mm diameter yttria-stabilized zirconium oxide beads. Used to grind soft animal and plant tissues. For use on FastPrep® instruments or any bead beater.

Tube contains 0.5 mm diameter yttria-stabilized zirconium oxide beads. Used to grind soft animal and plant tissues. For use on FastPrep® instruments or any bead beater.

Sample preparation tubes for use on FastPrep® instruments

Talniflumate, NeuroPure

Talniflumate, NeuroPure

Tamoxifen

Tamoxifen

Tamoxifen

Tamoxifen

Tandutinib, NeuroPure

Tandutinib, NeuroPure

Tandutinib, NeuroPure

Tannic Acid

Tannic Acid

Tannic Acid

Taps

Taps

Ready-to-use PCR mastermix

Kit for general PCR applications, includes Taq DNA Pol at 5 U/µL, dNTPs at 10 mM each, 10x Incubation Buffer with and without MgSO4, MgSO4 at 25 mM

Kit for general PCR applications, includes Taq DNA Pol at 5 U/µL, dNTPs at 10 mM each, 10x Incubation Buffer with and without MgSO4, MgSO4 at 25 mM

Kit for general PCR applications, includes Taq DNA Pol at 5 U/µL, dNTPs at 10 mM each, 10x Incubation Buffer with and without MgCl₂, MgCl₂ at 25 mM

Kit for general PCR applications, includes Taq DNA Pol at 5 U/µL, dNTPs at 10 mM each, 10x Incubation Buffer with and without MgSO4, MgSO4 at 25 mM

Kit for general PCR applications, includes Taq DNA Pol at 5 U/µL, dNTPs at 25 mM each, 10x Incubation Buffer with and without MgSO4, MgSO4 at 25 mM

Kit for general PCR applications, includes Taq DNA Pol at 5 U/µL, dNTPs at 25 mM each, 10x Incubation Buffer with and without MgSO4, MgSO4 at 25 mM

Kit for general PCR applications, includes Taq DNA Pol at 5 U/µL, dNTPs at 25 mM each, 10x Incubation Buffer with and without MgSO4, MgSO4 at 25 mM

Recombinant form – for general PCR applications
– Highly reproducible results
– Ideal for T/A cloning
– Ultra pure enzyme
– Highly processive

Recombinant form – for general PCR applications
– Highly reproducible results
– Ideal for T/A cloning
– Ultra pure enzyme
– Highly processive

Recombinant form – for general PCR applications
– Highly reproducible results
– Ideal for T/A cloning
– Ultra pure enzyme
– Highly processive

Recombinant form – for general PCR applications
– Highly reproducible results
– Ideal for T/A cloning
– Ultra pure enzyme
– Highly processive

Taq DNA Pol(5U/ul)

Recombinant form – for general PCR applications
– Highly reproducible results
– Ideal for T/A cloning
– Ultra pure enzyme
– Highly processive

Recombinant form – for general PCR applications
– Highly reproducible results
– Ideal for T/A cloning
– Ultra pure enzyme
– Highly processive

Recombinant form, for general PCR applications, includes direct loading incubation buffer.

Recombinant form, for general PCR applications, includes direct loading incubation buffer.

Recombinant form – for general PCR applications
– Highly reproducible results
– Ideal for T/A cloning
– Ultra pure enzyme
– Highly processive

Recombinant form – for general PCR applications
– Highly reproducible results
– Ideal for T/A cloning
– Ultra pure enzyme
– Highly processive

Taq Storage Buffer

Ready-to-use PCR mastermix

Ready-to-use mastermix for direct loading of PCR products on an agarose gel

Ready-to-use mastermix for direct loading of PCR products on an agarose gel

Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5’ end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3’ end.

Features include:
• Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
• Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
• Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
• Accurate—identify small fold-changes with high accuracy of quantitation
• Extensive content—over 1.8 million predesigned assays available for over 25 different species
• Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific’s bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
• Ideal for multiplexing—combine one FAM-labeled and one VIC-labeled assay to create a duplex assay for two different gene targets in the same qPCR well

Approximate ship time
Made to order: 4–6 days in North America, 6–10 days in Europe

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.

The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification. TaqMan Fast Advanced Cells-to-Ct cell lysis reagents are supplied with highest sensitivity Fast Advanced reverse transcription enzyme mix, 2x RT buffer, and best-in-class TaqMan Fast Advanced master mix. The TaqMan Fast Advanced Cells-to-Ct Kit gives you all the sensitivity you need in the fastest workflow available: get gene expressions analysis results from an entire plate of cells in about 80 minutes.

• Complete—optimized workflow includes cell lysis reagents with gDNA removal, new Fast Advanced RT enzyme mix, buffer, and new TaqMan Fast Advanced Master Mix
• Fast—7-minute sample prep, including DNase treatment, at room temperature
• Easy—lyse samples in a tube or directly in culture plates
• Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
• Efficient—contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples

Unique method eliminates the need to isolate RNA from cultured cells
The Cells-to-Ct method features a unique lysis reagent that lyses cells and protects RNA. DNA removal occurs simultaneously in the 5-minute lysis step to save time and improve ease of use. Lysis is then stopped by the addition of a “Stop Solution” that irreversibly binds to the lysis reagents.

The TaqMan Fast Advanced Cells-to-CT Kit contains reverse transcription (RT) reagents for cDNA synthesis and TaqMan Fast Advanced Master Mix for real-time PCR analysis. TaqMan primer/probe sets are sold separately.

Simple 7-minute sample preparation
The TaqMan Fast Advanced Cells-to-CT Kit is designed for 10–100,000 cultured cells/sample. Cells are washed in PBS and lysed in solution for five minutes at room temperature; DNase treatment can be performed simultaneously. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The lysates are now ready for reverse transcription or storage at –20°C for a short term or -80°C for a long term.

Maximize sensitivity of detection
Unlike some competitor kits that limit the amount of lysate in the RT reaction to 10%, the TaqMan Fast Advanced Cells-to-CT Kit can accommodate 45% of the total RT reaction volume as cell lysate. Additionally, cDNA can comprise up to 45% of the real-time PCR reaction volume. The large lysate volume in the optimized RT reaction, along with the large cDNA volume in the subsequent real-time PCR using the TaqMan Fast Advanced Master Mix, lead to maximum sensitivity. The master mix amplifies the target precisely and accurately, enabling the detection of small quantities of target, such as transcripts expressed at low levels.

Reduced sample loss and transfer error
Because samples can be processed directly in culture wells (96 or 384 wells), sample handling and the potential for sample loss or transfer error are minimized, facilitating rapid, high-throughput processing. Unlike old-fashioned, multi-step RNA isolation protocols, no heating, washing, or centrifugation steps are required. The kit greatly simplifies a laborious 30–60 minute process and reduces it to 7 minutes.

Compatible with gold standard TaqMan Gene Expression Assays
The TaqMan Fast Advanced Cells-to-CT Kit workflow enables unsurpassed gene expression evaluation with any of the >700,000 TaqMan Gene Expression Assays. This kit has been extensively tested for specificity with a broad selection of TaqMan Gene Expression Assays.

Proven performance, proven together
All components of the TaqMan Fast Advanced Cells-to-CT Kit have been optimized for consistent and reliable performance. This removes the guesswork involved in assembling separate sample preparation, RT, and real-time PCR kits. And the TaqMan Fast Advanced Cells-to-CT Kit has been validated with TaqMan Gene Expression Assays for added quality assurance, giving you the sensitivity you need in the fastest sample-to-answer workflow available.

The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification. TaqMan Fast Advanced Cells-to-Ct cell lysis reagents are supplied with highest sensitivity Fast Advanced reverse transcription enzyme mix, 2x RT buffer, and best-in-class TaqMan Fast Advanced master mix. The TaqMan Fast Advanced Cells-to-Ct Kit gives you all the sensitivity you need in the fastest workflow available: get gene expressions analysis results from an entire plate of cells in about 80 minutes.

• Complete—optimized workflow includes cell lysis reagents with gDNA removal, new Fast Advanced RT enzyme mix, buffer, and new TaqMan Fast Advanced Master Mix
• Fast—7-minute sample prep, including DNase treatment, at room temperature
• Easy—lyse samples in a tube or directly in culture plates
• Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
• Efficient—contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples

Unique method eliminates the need to isolate RNA from cultured cells
The Cells-to-Ct method features a unique lysis reagent that lyses cells and protects RNA. DNA removal occurs simultaneously in the 5-minute lysis step to save time and improve ease of use. Lysis is then stopped by the addition of a “Stop Solution” that irreversibly binds to the lysis reagents.

The TaqMan Fast Advanced Cells-to-CT Kit contains reverse transcription (RT) reagents for cDNA synthesis and TaqMan Fast Advanced Master Mix for real-time PCR analysis. TaqMan primer/probe sets are sold separately.

Simple 7-minute sample preparation
The TaqMan Fast Advanced Cells-to-CT Kit is designed for 10–100,000 cultured cells/sample. Cells are washed in PBS and lysed in solution for five minutes at room temperature; DNase treatment can be performed simultaneously. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The lysates are now ready for reverse transcription or storage at –20°C for a short term or -80°C for a long term.

Maximize sensitivity of detection
Unlike some competitor kits that limit the amount of lysate in the RT reaction to 10%, the TaqMan Fast Advanced Cells-to-CT Kit can accommodate 45% of the total RT reaction volume as cell lysate. Additionally, cDNA can comprise up to 45% of the real-time PCR reaction volume. The large lysate volume in the optimized RT reaction, along with the large cDNA volume in the subsequent real-time PCR using the TaqMan Fast Advanced Master Mix, lead to maximum sensitivity. The master mix amplifies the target precisely and accurately, enabling the detection of small quantities of target, such as transcripts expressed at low levels.

Reduced sample loss and transfer error
Because samples can be processed directly in culture wells (96 or 384 wells), sample handling and the potential for sample loss or transfer error are minimized, facilitating rapid, high-throughput processing. Unlike old-fashioned, multi-step RNA isolation protocols, no heating, washing, or centrifugation steps are required. The kit greatly simplifies a laborious 30–60 minute process and reduces it to 7 minutes.

Compatible with gold standard TaqMan Gene Expression Assays
The TaqMan Fast Advanced Cells-to-CT Kit workflow enables unsurpassed gene expression evaluation with any of the >700,000 TaqMan Gene Expression Assays. This kit has been extensively tested for specificity with a broad selection of TaqMan Gene Expression Assays.

Proven performance, proven together
All components of the TaqMan Fast Advanced Cells-to-CT Kit have been optimized for consistent and reliable performance. This removes the guesswork involved in assembling separate sample preparation, RT, and real-time PCR kits. And the TaqMan Fast Advanced Cells-to-CT Kit has been validated with TaqMan Gene Expression Assays for added quality assurance, giving you the sensitivity you need in the fastest sample-to-answer workflow available.