All Products shortcode with no Uncategorized products. – (SHOP test speed)

GIBBERELLIC ACID, 90%

CAS Number: 77-06-5

CAS Number: 77-06-5

CAS Number: 77-06-5

Gibberellins A4 + A7, PhytoPure

Gibberellins A4 + A7, PhytoPure

Gibco PBS, pH 7.2, is a balanced salt solution widely used in various cell culture applications to maintain a stable pH and osmotic balance, making it ideal as a wash, transport, and dilution buffer.

• • Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G solution is now routinely used with other basal media to support a wide variety of cell types in the presence of 2 to 4% FBS

n

• • Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids

n

• • Transferrin is an iron carrier and it may also help to reduce toxic levels of oxygen radicals and peroxide

n

• • Selenium, as sodium selenite, is a cofactor for glutathione peroxidase and other proteins, and is used as an antioxidant in media

n

Human EGF Recombinant Protein for Western Blot, IHC, ELISA, Ctrl, FN

nEGF has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin.

• • This product is provided as lyophilized and carrier-free

n

• • High purity: no interference from other proteins or contaminants

n

• • High biological activity: more results with less protein

n

• • Proven compatibility: to help ensure growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein

n

• • High biological activity: in-house activity testing of recombinant proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, anti-viral, and protease assays; specific activity is determined by the dose dependent proliferation of mouse BALB/3T3 cells

n

• • Mercaptoethanol is not stable in solution. so most protocols require daily supplementation

n

• • Concentration of 55mM in Dulbecco’s Phosphate Buffered Saline (DPBS)

n

• • Manufactured at a cGMP compliant facility, located in Grand Island, New York, registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards

n

• • Manufactured at a cGMP-compliant facility located in Grand Island, New York, registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards

Cell Culture, Clinical, Clinical and Translational Research, Cytogenetics, Karyotyping, Mammalian Cell Culture

The most cited neuronal cell culture supplement, B-27 Supplement is an optimized serum-free supplement used to support the low- or high-density growth and short- or long-term viability of embryonic, post-natal, and adult hippocampal and other CNS neurons. B-27 Supplement is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.

• • Provided as 50X liquid

• • Used with Neurobasal™ Medium for neuronal cell culture

• • Tested in growth assay utilizing rat (Sprague Dawley) embryonic hippocampal neurons

• • Tested also for endotoxin at a 1X concentration

BHI broth is used for the cultivation of a wide variety of microorganisms, including bacteria (streptococci, pneumococci and meningococci), yeasts and molds. It serves as a base for supplemented media containing 0.1% agar, Fildes enrichment or 6.5% sodium chloride.

• To use dehydrated product: suspend 37g in 1L of purified water
• Displays a good cultural response to Neisseria meningitides 13090, Streptococcus pneumonia 6305 and Streptococcus pyogenes 19615
• pH (3.7% solution, 25°C): 7.4 ± 0.2

Certifications

AOAC, BAM, CCAM, CLSI, CMPH2, COMPF, EPA, ISO, MCM9, SMD, SMWW, USDA

• An animal origin (AO) acid hydrolysis of casein
• Less processing results in higher sodium chloride and iron content
• It has successfully be used in the production of a number of animal health vaccines
• Bacto™ Casamino Acids, technical has been shown to support the growth of Bordetella, Haemophilus, and Vibrio species
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

• Bacto Malt Extract is the water-soluble portion of malted barley
• Due to the extraction process, this animal origin-free supplement provides a very high carbohydrate content to any medium
• It has been used successfully in microbial fermentation applications
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

• Bacto Neopeptone is an animal origin (AO) enzymatic digest of bovine (milk) and porcine protein
• This nutritional supplement provides vitamins, nucleotides, minerals, and peptides in a variety of sizes to any culture formulation
• It has been used as a component of culture media in the cultivation of human pathogens, notably Bordetella pertussis and group A streptococci
• Also used with Borrelia burgdorferi and Clostridium species, to produce vaccines for animal health application
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

Applications of Bacto Neopeptone include:

• Recommended for use in media for detection of fungi
• Reported to provide nutrients for support of spirochetes and protozoa
• Microbial fermentation
• Production of vaccines for animal health applications

• Bacto Proteose Peptone No. 3 is a member of the Bacto Proteose Peptones, which are enzymatic digests of protein
• Studies show that no single peptone is the most suitable nitrogen source for every microbiological application
• These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone
• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture media
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

• Bacto Proteose Peptone No. 3 is a member of the Bacto Proteose Peptones, which are enzymatic digests of protein
• Studies show that no single peptone is the most suitable nitrogen source for every microbiological application
• These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone
• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture media
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

• Bacto Proteose Peptone No. 3 is a member of the Bacto Proteose Peptones, which are enzymatic digests of protein
• Studies show that no single peptone is the most suitable nitrogen source for every microbiological application
• These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone
• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture media
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

• Bacto Proteose Peptones are enzymatic digests of protein
• Studies show that no single peptone is the most suitable nitrogen source for every microbiological application
• These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone.
• Bacto Proteose Peptone is used in preparing microbiological culture media and in producing bacterial toxins
• Store at 2° to 25°C in cool, dry conditions.

This supplement is a mixture of peptides, amino acids, and carbohydrates, both simple and complex. Customers have successfully used Gibco™ Bacto™ TC Lactalbumin Hydrolysate to support the growth of many mammalian, bacterial, and insect cell types, and it has been used to produce both recombinant proteins and animal health vaccines. Customers have also used this supplement as an amino acid supplement for mammalian cell culture media.

• Bacto Tryptone is an animal origin (AO) pancreatic digest of casein
• It is included in standard methods manual applications and is listed in the Reagent section of the United States Pharmacopeia as meeting the specifications for a pancreatic digest of casein, a component in many of the media listed
• The nutritional profile of this supplement is unique in that it provides a range of nutrients
• Customers have used this supplement in fermentation applications and shown that it successfully supports the growth of many bacterial species during the production of animal health vaccines
• This includes Escherichia coli, and Fusobacterium and Agrobacterium species when used together with Bacto Yeast Extract
• Store in cool, dry conditions in well-sealed containers at 2° to 25°C

Features:

• Pancreatic digest of casein
• Developed while investigating a peptone particularly suitable for the elaboration of indole by bacteria
• Animal origin nutritional supplement

Applications of Bacto Tryptone include:

• Works well in fermentation applications
• Use with common organisms such as Escherichia coli, as well as uncommon organisms such as the diatom Nitzschia laevis

• Bacto Tryptone is an animal origin (AO) pancreatic digest of casein
• It is included in standard methods manual applications and is listed in the Reagent section of the United States Pharmacopeia as meeting the specifications for a pancreatic digest of casein, a component in many of the media listed
• The nutritional profile of this supplement is unique in that it provides a range of nutrients
• Customers have used this supplement in fermentation applications and shown that it successfully supports the growth of many bacterial species during the production of animal health vaccines
• This includes Escherichia coli, and Fusobacterium and Agrobacterium species when used together with Bacto Yeast Extract
• Store in cool, dry conditions in well-sealed containers at 2° to 25°C

Features:

• Pancreatic digest of casein
• Developed while investigating a peptone particularly suitable for the elaboration of indole by bacteria
• Animal origin nutritional supplement

Applications of Bacto Tryptone include:

• Works well in fermentation applications
• Use with common organisms such as Escherichia coli, as well as uncommon organisms such as the diatom Nitzschia laevis

• Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells, derived from primary grown baker’s yeast
• These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications
• Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation
• Bacto Yeast Extract is one of the most complete and versatile fermentation supplements available
• It is an important ingredient for the microbiological assay of vitamins
• Yeast extract is also of value in the assay of antibiotics
• B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract
• Bacto Yeast Extract, Technical, was developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

Related yeast extract products include:

• Gibco Yeast Extract – Developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF – Ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g
• Difco Yeast Extract Low-Dusting (LD) – Designed to limit the amount of dust generated compared to other spray-dried yeast extracts.

• Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells, derived from primary grown baker’s yeast
• These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications
• Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation
• Bacto Yeast Extract is one of the most complete and versatile fermentation supplements available
• It is an important ingredient for the microbiological assay of vitamins
• Yeast extract is also of value in the assay of antibiotics
• B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract
• Bacto Yeast Extract, Technical, was developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

Related yeast extract products include:

• Gibco Yeast Extract – Developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF – Ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g
• Difco Yeast Extract Low-Dusting (LD) – Designed to limit the amount of dust generated compared to other spray-dried yeast extracts.

• Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells, derived from primary grown baker’s yeast
• These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications
• Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation
• Bacto Yeast Extract is one of the most complete and versatile fermentation supplements available
• It is an important ingredient for the microbiological assay of vitamins
• Yeast extract is also of value in the assay of antibiotics
• B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract
• Bacto Yeast Extract, Technical, was developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

Related yeast extract products include:

• Gibco Yeast Extract – Developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF – Ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g
• Difco Yeast Extract Low-Dusting (LD) – Designed to limit the amount of dust generated compared to other spray-dried yeast extracts

• Concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells derived from primary grown baker’s yeast
• These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications
• Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation
• Bacto Yeast Extract is an important ingredient for the microbiological assay of vitamins
• B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract
• Developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

The combination of pancreatic digest of casein and yeast extract provides synergistic nutritional benefits that are not provided by the components alone, resulting in a unique nutritional profile. Customers have shown the combined use of these two peptones, when supplied as Biosate Peptone, improves toxin production with Clostridial species for vaccines, such as Clostridium botulinum and Clostridium perfringens.

Gibco™ BiTek™ Fermentation Broth is a bacterial fermentation formulated with ingredients that exhibit superior consistency, performance, and solubility.

Developed while investigating a peptone particularly suitable for the elaboration of indole by bacteria. The final product goes through fewer refinement steps during processing than Gibco™ Bacto™ Tryptone.

Promotes an acceleration in the monoclonal antibody secretion rates of cells in a batch mode for performance in hollow fiber and stir tank systems.

• Suitable for use in perfusion systems
• Shown to increase purified monoclonal antibody yields by 5 to 25 times over classical basal media with similar supplementation
• Reduce media consumption and labor cost, reduce requirements for media storage and preparation time, decrease labor required to perform continuous feeding and decrease contamination because of decreased handling of the culture
• Concentration step is eliminated in most cases, and final bulk harvest is reduced, producing less material to run through the purification process
• Only minimal adaptation is needed
• Hybridomas grown in MAb Basal Medium can be supplemented in the same way as those grown in traditional basal media
• MAb Serum-Free and Animal-Free are complete media and do not require serum supplementation; Basal Medium does require serum supplementation

GIBCO OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free, xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bottle) and OpTmizer™ T-Cell Expansion Supplement (26 mL), which are mixed together prior to use.

• • Maintains similar phenotype and function (e.g., cytokine secretion profile) of polyclonally activated and cultured T cells as observed for conventional serum supplemented medium

n

• • Consistent performance in supporting T cell expansion

n

• • Supports high density T cell culture (e.g., >3×10⁶ CD3+ T cells/mL) in static culture
    
    With OpTmizer™ T-Cell Expansion SFM, we offer both basic and clinical research communities a new tool to enhance their success in growing human T-cells. It is specially formulated to allow for superior cell growth & viability, less variability and more consistent results, and easier transition from research to clinical applications.
    
    Workflow benefits:

n

• • Minimizing the need to handle purchasing, storage, qualification of serum and thus reducing variability and costs

n

• • More rapid T cell expansion, thus reducing cycle time and improving productivity

n

• • Complete serum-free, xeno-free medium, allowing for a seamless transition from research to clinical applications and minimizing regulatory workload
    
    “This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”

n

• Prepared according to the method described by Mueller and Miller
• Supplemented with growth factors, cystine, maltose and iron and inorganic salts
• Successful in fermentation for Pneumococcal, Diptheria, Pertussis, Tetanus and Zoster vaccines
• Support the growth of Bordetella, Corynebacterium, Pseudomonas, Haemophilus and Escherichia
• Dehydrated format with Peptones / Hydrolysates
• DNR (Latex) free

• Prepared according to the method described by Mueller and Miller
• Supplemented with growth factors, cystine, maltose and iron and inorganic salts
• Successful in fermentation for Pneumococcal, Diptheria, Pertussis, Tetanus and Zoster vaccines
• Support the growth of Bordetella, Corynebacterium, Pseudomonas, Haemophilus and Escherichia
• Dehydrated format with Peptones / Hydrolysates
• DNR (Latex) free