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A complete media used in the cultivation of microorganisms from the class Mollicutes. Used in animal health applications for the propagation of Mycoplasma species such as Mycoplasma mycoides, Mycoplasma gallisepticum, and others for vaccine production.

• An alternative carbohydrate profile to yeast supplements for mammalian cell processes, such as CHO
• High concentrations of vitamins and carbohydrates
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

Difco Tryptone Peptone is an animal origin (AO) pancreatic digest of casein. In addition to providing a unique nutritional profile, it has a higher nitrogen content than our other casein products. Tryptone Peptone supports the growth of many different types of bacteria and fungi, including fastidious organisms and in toxin production. Customers have used this supplement in the development of animal health vaccines, such as Clostridial vaccines, and with human health Botox-like products produced from Clostridium botulinum.

Features:
• Animal origin, pancreatic digest of casein
• Primary nitrogen source in trypticase soy broth and agar
• Referenced in Official Methods of Analysis of AOAC International
• Supports the growth of many different types of bacteria and fungi

Applications of Tryptone Peptone include:
• Recommended for use in media formulations when good growth of fungi and bacteria is required
• Used in the development of animal health vaccines and with human health Botox-like products

• • Dulbecco’s Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells

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• • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12

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• • DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium

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• • It was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate

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Modified as follows

• • With: low glucose, sodium pyruvate

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• • Without: L-glutamine, phenol red, HEPES

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Using DMEM

• • DMEM contains no proteins, lipids, or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)

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• • It uses a sodium bicarbonate buffer system (3.7 g/L) and requires a 5-10% CO₂ environment to maintain physiological pH

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Description

• • Dulbecco’s Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents

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• • Formulations with calcium and magnesium are generally used as transport media or for reagent preparation

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• • Store at 15°C to 30°C

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Modified as follows

• • With: calcium, magnesium, glucose, sodium pyruvate

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• • Without: phenol red

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Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression.

• • Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL

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• • Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent

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• • Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein

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• • Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10 L in disposable bioreactors

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• • Does not contain phenol red

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• • Milligram to multigram yields of recombinant protein per liter of culture in 5 to 14 days post-transfection, which are frequently higher than transient 293-based systems

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• • Cost-effective transient expression in CHO cells, allowing scientists developing protein biotherapeutics to work with CHO-expressed proteins from start to finish in the drug development process

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• • A robust alternative mammalian transient expression host, allowing for production of proteins that are difficult to express in HEK293 cells

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• • Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows

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• • Scalable expression for cultures from <1 mL to >10 L, thus generating the amount of protein needed for your application

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• • Achieves greater than 90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression

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• • Requires one-third to one-half less plasmid DNA than other transfection reagents while achieving superior expression performance

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• • Enables options for three transient expression protocols for maximal work flexibility while still fitting into the same overall workflow used by other transient expression systems, such as FreeStyle™ MAX CHO and Expi293™

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• • Delivers robust and reproducible transfection results, giving you greater confidence in your results

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• • Demonstrates linear scalability of transfections from culture volumes of <1 mL to >10 L

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Description

• • Suitable for both adherent and suspension culture of mammalian cells, with no adaptation required

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• • Offered as a 200mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl

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• • Compared to L-glutamine: Minimizes toxic ammonia build-up; Improves cell viability and growth; Remains stable across a wide range of temperaturesI

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• • Cells cleave the dipeptide bond to release L-glutamine as needed, preventing waste build-up and maintaining a fresh supply of L-glutamine during long-term cultureI

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• • Cultures with lower levels of ammonia show improved cell viability

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• • In addition, culture conditions are more controlled with the consistent concentration not possible with L-glutamineI

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• • Room-temperature stable and ready to use

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• • Manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK, registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard

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Cell Culture, Clinical, Clinical and Translational Research, Cytogenetics, Karyotyping, Mammalian Cell Culture.

Description

• • Suitable for both adherent and suspension culture of mammalian cells, with no adaptation required

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• • Offered as a 200mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl

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• • Compared to L-glutamine: Minimizes toxic ammonia build-up; Improves cell viability and growth; Remains stable across a wide range of temperaturesI

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• • Cells cleave the dipeptide bond to release L-glutamine as needed, preventing waste build-up and maintaining a fresh supply of L-glutamine during long-term cultureI

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• • Cultures with lower levels of ammonia show improved cell viability

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• • In addition, culture conditions are more controlled with the consistent concentration not possible with L-glutamineI

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• • Room-temperature stable and ready to use

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• • Manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK, registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard

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Cell Culture, Clinical, Clinical and Translational Research, Cytogenetics, Karyotyping, Mammalian Cell Culture

• • HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media

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• • The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO₂ incubator

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• • A good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable

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• • It also has very low visible and UV light absorbance

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• • Store at 2°C to 8°C

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• • IMDM (Iscove’s Modified Dulbecco’s Medium) is well suited for rapidly proliferating, high-density cell cultures including Jurkat, COS-7, and macrophage cells

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• • Store at 2°C to 8°C, protected from light

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Modified as follows:

• • With: GlutaMAX™, phenol red

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• • Without: a-thioglycerol, 2-mercaptoethanol

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Using IMDM

• • IMDM, a modification of Dulbecco’s Modified Eagle Medium, includes selenium as well as additional amino acids and vitamins

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• • This unique medium lacks iron, with potassium nitrate replacing ferric nitrate

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• • IMDM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format

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• • IMDM contains no proteins, lipids, or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)

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• • It uses a sodium bicarbonate buffer system (3.024 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH

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Order Info

Shipping Condition: Room Temperature

L-Glutamine is an amino acid that is required for cell culture. L-Glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production. Unlike most other amino acids, L-glutamine is not stable in solution. The rate at which degradation proceeds is a function of time, temperature, and pH.

• • Optimal concentration is dependent upon the cell type and medium used to culture the cells, but generally falls in the range of 2 to 6 mM

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• • Manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK, registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard

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Bioproduction, Cell Culture, Cloning, Mammalian Cell Culture, Transformation, Viral Vaccine Production.

Neurobasal Plus Medium is a neuronal basal medium that provides the highest rates of in vitro survival of primary rodent and human stem cell-derived neurons when used with B-27 Plus Supplement. Neurobasal Plus Medium features an optimized formulation, upgraded manufacturing process, and more stringent quality control of raw materials and final product. These improvements enable the highest rates of neuronal cell survival, improved electrophysiological activity, and enhanced functional maturity versus other neuronal cell culture media systems.nnNeurobasal Plus Medium, when combined with B-27 Plus Supplement, offers:

• • At least 50% higher survival rate of primary rat neurons compared to classic Neurobasal Medium and B-27 Supplement

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• • Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems

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• • Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium

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• • Accelerates neurite outgrowth and increases the number of synaptic-positive puncta

Opti-MEMI medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts.

• • Opti-MEM I is modified as follows: with L-glutamine, phenol red

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• • Unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements

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• • Additional components allow for reduction in serum supplementation by at least 50%

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• • Uses sodium bicarbonate buffer system (2.4g/L), and therefore requires a 5-10% CO₂ environment to maintain physiological pH

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• • Sustainable packaging

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Cell Culture, Mammalian Cell Culture, Plasmid Transfection, RNAi Transfection, Synthetic siRNA Transfection, Transfection, shRNA and miR RNAi Plasmid Transfection.

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution (see protocol for details).

We offer a variety of Gibco™ PBS formulations for a range of cell culture applications.

PBS (phosphate buffered saline) is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. This PBS is manufactured without calcium, magnesium or phenol red. The complete formulation is available.

• • Animal origin-free

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• • Sustainable packaging

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Cell Culture, Mammalian Cell Culture

PBS (phosphate buffered saline) is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. This PBS is manufactured without calcium, magnesium or phenol red. The complete formulation is available.

• • Animal origin-free

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• • Sustainable packaging

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Cell Culture, Mammalian Cell Culture

PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. We offer a variety of PBS formulations for a range of cell culture applications. Find the right formulation using the media selector tool.

This PBS is manufactured as follows:
Without: Calcium, Magnesium, Phenol Red

• An animal origin-free (AOF) enzymatic digest of soy
• A nutritious and excellent carbohydrate source due to the naturally high concentrations of vitamins and carbohydrates in soy
• Customers have successfully used this supplement in CHO-based production of monoclonal antibodies for oncological and rheumatological therapeutics
• Also used with many different microbial cell types to produce animal health vaccines for swine, such as Clostridial vaccines

Applications of Phytone Peptone include:

• It is an excellent plant peptone for the cultivation of fungi and fastidious types of bacteria, such as members of the Clostridium and Neisseria genera
• It has been used in mammalian cell culture applications due to its high carbohydrate content

• • Used to reduce foaming in stirred cultures and reduce cell attachment to glass

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• • Provided at concentration of 10% and effective at working concentration of 0.1%

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• • Manufactured at a cGMP compliant facility, located in Grand Island, New York, registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards

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Cell Culture, Clinical, Clinical and Translational Research, Cytogenetics, Karyotyping, Mammalian Cell Culture

For many years, researchers have used a common recipe for making freezing medium: High-glucose Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% serum and 10% DMSO. Recovery Cell Culture Freezing Medium is an optimized version of this classic formulation, using Gibco Bovine Serum and Fetal Bovine Serum to provide improved cell recovery and viability after thawing.

• • Suitable for a wide variety of mammalian cells

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• • Can be used to freeze most mammalian cells cultured in DMEM, DMEM/F-12, MEM, RPMI 1640, and Ham’s F-12

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• • Quality tested for pH, osmolality, sterility, and endotoxin

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• • Each lot is performance tested using CHO-K1 cells

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Cell Counting, Viability & Cryopreservation, Cell Culture, Mammalian Cell Culture, Gene Expression Analysis & Genotyping, In Vitro Transcription, PCR & Real-Time PCR

A chemically defined supplement formulation to complement a variety of base media. This supplement was developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors. Developed for use in CHO mammalian culture systems and can be used either as a media supplement or a culture system feed.

A chemically defined supplement formulation to complement a variety of base media. This supplement was developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors. Developed for use in CHO mammalian culture systems and can be used either as a media supplement or a culture system feed.

An animal origin-free (AOF) protein-free chemically defined supplement. It can be used in batch and fed batch mode in cell culture processes. All components are chemically defined with known molecular formulae and structures and meet our stringent Animal Origin-Free (AOF) specification. Formulated without glucose, L-glutamine, and sodium bicarbonate. Due to the balanced nutritional nature of this supplement, rich in amino acids, it has also been used in bacterial applications.

A chemically defined supplement formulation to complement a variety of base media. This supplement was developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors. Developed for use in CHO mammalian culture systems and can be used either as a media supplement or a culture system feed.

Sodium pyruvate is commonly added to cell culture media as a carbon source in addition to glucose. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement for all cell cultures. However, if cells have been grown in medium that is supplemented with sodium pyruvate, consider continuing use of the supplement as cell growth may lag without it.

• • Final concentration of sodium pyruvate used in most cell culture media is 1mM

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• • Manufactured at a cGMP compliant facility, located in Grand Island, New York, registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards

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n nnCell Counting and Viability, Cell Counting, Viability, and Cryopreservation, Cell Culture, Mammalian Cell Culturen

Order Info

Shipping Condition: Room Temperature

Gibco TrypLE Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, TrypLE Select is:

• Gentle on cells—maintains cell health for reproducible results
• Room-temperature stable—convenient to use
• Easy to use—substitutes directly into existing protocols with no inactivation required
• Animal origin free (AOF)—pure, consistent, and effective

This TrypLE Select is modified as follows:

With
• EDTA

Without
• Phenol Red

Gentle on cells
TrypLE Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE Select’s exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room-temperature stable
TrypLE Select is room-temperature stable and ready to use. TrypLE Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Select, avoiding the need for trypsin inhibitors, such as FBS.

Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE Select is free of animal-derived components and is formulated on dedicated animal origin-free equipment.

Product Intended Use
Customers using TrypLE Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
TrypLE Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Acidicase Peptone is a hydrochloric acid hydrolysate of casein. The manufacturing process produces a casein hydrolysate, which has a high salt content of approximately 37% and nitrogen content of approximately 8%. The hydrolysis of the casein, a milk protein rich in amino acid nitrogen, is carried out until all the nitrogen is converted to amino acids or other compounds of relative simplicity. Casein contains little cystine and is deficient in tryptophan, which is destroyed by the acid treatment.

Features:
• Hydrochloric acid hydrolysate of casein
• Animal origin (AO) nutritional supplement
• Milk protein rich in amino acid nitrogen

Applications of Acidicase Peptone include:
• Intended for use as a nutritional supplement in vitamin assays, susceptibility testing, and other laboratory media and microbial fermentation in which the high salt content will not interfere

Bacto Casamino Acids is an animal origin (AO) acid hydrolysis of casein. This supplement contains low salt and iron content making it an excellent supplement for media formulations where nitrogen requirements are minimal. This supplement is successfully used in fermentation applications for pneumococcal, diphtheria, pertussis, tetanus, and zoster vaccines. It maintains many of the nutritional benefits of hydrolyzed casein, but is further supplemented with growth factors, cystine, maltose, and an optimum amount of iron and inorganic salts.

Customers have used Bacto Casamino Acids to support the growth of Bordetella, Corynebacterium, Pseudomonas, Haemophilus, and Escherichia species. Additionally, together with Bacto Yeast Extract, technical, customers have used this supplement successfully in the production of animal and human health vaccines.

Bacto Casamino Acids, technical, is an animal origin (AO) acid hydrolysis of casein. In contrast to the Bacto Casamino Acids, this supplement has been processed less and, as a result, has higher sodium chloride and iron content. Customers have successfully used Bacto Casamino Acids, technical, in the production of a number of animal health vaccines, and it has been shown to support the growth of Bordetella, Haemophilus, and Vibrio species.

Bacto Malt Extract is the water-soluble portion of malted barley. Due to the extraction process, this animal origin-free supplement provides a very high carbohydrate content to any medium. Bacto Malt Extract has been used successfully in microbial fermentation applications.

Bacto Neopeptone is an animal origin (AO) enzymatic digest of bovine (milk) and porcine protein. This nutritional supplement provides vitamins, nucleotides, minerals, and peptides in a variety of sizes to any culture formulation. Customers have used Bacto Neopeptone as a component of culture media in the cultivation of human pathogens, notably Bordetella pertussis and group A streptococci, as well as Borrelia burgdorferi and Clostridium species, to produce vaccines for animal health applications.

Features:
• Enzymatic digest of bovine (milk) and porcine protein
• Animal origin nutritional supplement
• Contains a wide variety of peptide sizes in combination with vitamins, nucleotides, and minerals

Applications of Bacto Neopeptone include:
• Recommended for use in media for detection of fungi
• Reported to provide nutrients for support of spirochetes and protozoa
• Microbial fermentation
• Production of vaccines for animal health applications

Bacto Peptone is an animal origin (AO) enzymatic digest of bovine and porcine animal proteins. The nutritive value of Bacto Peptone is largely dependent on a combination of a negligible quantity of proteases, high concentration of complex constituents, and a unique amino acid content that makes this peptone a strong nitrogen source. Customers have successfully used this supplement with a variety of cell types including Saccharomyces cerevisiae, Escherichia coli, Pasteurella multocida, Vibrio cholerae, and porcine kidney cells to produce biotherapeutics and vaccines for animal health applications.

Features:
• A strong nitrogen source
• Unique amino acid content
• High concentration of complex constituents

Applications of Bacto Peptone include:
• Vaccines for animal health applications
• Production of biotherapeutics
• Mammalian cell culture
• Microbial fermentation

Bacto Proteose Peptones are enzymatic digests of protein. Studies of peptic digests of animal tissue prepared under varying digestion parameters demonstrated that no single peptone is the most suitable nitrogen source for every microbiological application. These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone.

• Bacto Proteose Peptone is used in preparing microbiological culture media and in producing bacterial toxins
• Bacto Proteose Peptone No. 2 is used in preparing microbiological culture media
• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture media
• Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone. It offers the same beneficial nutrients as Bacto Proteose Peptone for growth promotion and toxin production in a wide range of fastidious microorganisms.

Bacto Proteose Peptone No. 2 is member of the Bacto Proteose Peptones, which are enzymatic digests of protein. Studies of peptic digests of animal tissue prepared under varying digestion parameters demonstrated that no single peptone is the most suitable nitrogen source for every microbiological application. These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone.nn• Bacto Proteose Peptone is used in preparing microbiological culture media and in producing bacterial toxinsn• Bacto Proteose Peptone No. 2 is used in preparing microbiological culture median• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture median• Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone. It offers the same beneficial nutrients as Bacto Proteose Peptone for growth promotion and toxin production in a wide range of fastidious microorganisms.

Bacto Proteose Peptone No. 3 is a member of the Bacto Proteose Peptones, which are enzymatic digests of protein. Studies of peptic digests of animal tissue prepared under varying digestion parameters demonstrated that no single peptone is the most suitable nitrogen source for every microbiological application. These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone.

• Bacto Proteose Peptone is used in preparing microbiological culture media and in producing bacterial toxins
• Bacto Proteose Peptone No. 2 is used in preparing microbiological culture media
• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture media
• Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone. It offers the same beneficial nutrients as Bacto Proteose Peptone for growth promotion and toxin production in a wide range of fastidious microorganisms.

Bacto Soytone is an enzymatic digest of soy that uses an animal origin (AO) (porcine) enzyme for digestion. The naturally high concentrations of vitamins and carbohydrates found in soy make Bacto Soytone a nutritious and excellent carbohydrate source for those not concerned about the processing step involving an animal-based material. This supplement offers a unique nutritional profile as compared to other soy products, and so can be a valuable alternative to consider for supplementing both mammalian and microbial processes.

Bacto TC Lactalbumin Hydrolysate, an animal origin peptone, is the enzymatically hydrolyzed protein portion of milk whey. This supplement is a mixture of peptides, amino acids, and carbohydrates, both simple and complex. Customers have successfully used Bacto TC Lactalbumin Hydrolysate to support the growth of many mammalian, bacterial, and insect cell types, and it has been used to produce both recombinant proteins and animal health vaccines. Customers have also used this supplement as an amino acid supplement for mammalian cell culture media.

Bacto Tryptone is an animal origin (AO) pancreatic digest of casein. It is included in standard methods manual applications and is listed in the Reagent section of the United States Pharmacopeia as meeting the specifications for a pancreatic digest of casein, a component in many of the media listed. The nutritional profile of this supplement is unique in that it provides a range of nutrients. Customers have used this supplement in fermentation applications and shown that it successfully supports the growth of many bacterial species during the production of animal health vaccines, including Escherichia coli, and Fusobacterium and Agrobacterium species when used together with Bacto Yeast Extract.

Features:
• Pancreatic digest of casein
• Developed while investigating a peptone particularly suitable for the elaboration of indole by bacteria
• Animal origin nutritional supplement

Applications of Bacto Tryptone include:
• Works well in fermentation applications
• Use with common organisms such as Escherichia coli, as well as uncommon organisms such as the diatom Nitzschia laevis

Bacto Tryptose is a mixed enzymatic hydrolysate with distinctive nutritional properties. The digestive process of tryptose results in assorted peptides of higher molecular weight suitable for long-chain amino acid requirements.

Features:
• Reported as beneficial for cell culture applications
• Animal origin (AO) nutritional supplement

Applications of Bacto Tryptose include:
• Tryptose is very useful for cultivation of streptococci, pneumococci, meningococci,
and other fastidious organisms.
• Tryptose Peptone is often used as a biomass enhancer for recombinant Escherichia coli production

Bacto Tryptose Phosphate Broth is an infusion-free buffered medium recommended for the cultivation of fastidious, pathogenic microorganisms. It is valuable in tissue culture procedures where the peptone content is considered to be a stimulating factor for cells.

Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells. They are derived from primary grown baker’s yeast. These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications. Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation.

Bacto Yeast Extract is one of the most complete and versatile fermentation supplements available. It is an important ingredient for the microbiological assay of vitamins. Yeast extract is also of value in the assay of antibiotics. B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract. Bacto Yeast Extract, Technical, was developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics.

Related yeast extract products:
• Gibco Yeast Extract—developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF—ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g.
• Difco Yeast Extract Low-Dusting (LD)—designed to limit the amount of dust generated compared to other spray-dried yeast extracts

• • Concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells derived from primary grown baker’s yeast

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• • These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications

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• • Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation

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• • Bacto Yeast Extract is an important ingredient for the microbiological assay of vitamins

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• • B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract

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• • Developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics

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• • Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

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Biosate Peptone is an animal origin (AO) mixed hydrolysate comprised of 65% pancreatic digest of casein and 35% yeast extract. This combination provides synergistic nutritional benefits that are not provided by the components alone, resulting in a unique nutritional profile. Customers have shown the combined use of these two peptones, when supplied as Biosate Peptone, improves toxin production with Clostridial species for vaccines, such as Clostridium botulinum and Clostridium perfringens.

Features:
• Hydrochloric acid hydrolysate of casein
• Animal origin nutritional supplement
• Milk protein rich in amino acid nitrogen

Applications of Biosate Peptone include:
• Intended for use as a nutritional supplement in vitamin assays, susceptibility testing, and other laboratory media, and microbial fermentation in which the high salt content will not interfere