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This broad-spectrum Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases. The 100X cocktails, available in regular and EDTA-free formulations and several package formats, are refrigerator-stable liquids that are very easy to use for nearly any sample or experimental scale. Highlights:

• • Complete protection all-in-one cocktail contains both protease and phosphatase inhibitors

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• • Multiple package sizes choose from four convenient packages sizes to match nearly any scale

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• • Convenient the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats

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• • Easy storage aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails

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• • Compatible can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift

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• • Validated fully compatible with Thermo Scientific Pierce™ Cell Lysis Buffers

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nIncludes:nnFormulations with and without supplementary vial of EDTA for metalloprotease inhibition, both of which are offered in four package sizes.

This broad-spectrum Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases. The 100X cocktails, available in regular and EDTA-free formulations and several package formats, are refrigerator-stable liquids that are very easy to use for nearly any sample or experimental scale. Highlights:

• • Complete protection all-in-one cocktail contains both protease and phosphatase inhibitors

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• • Multiple package sizes choose from four convenient packages sizes to match nearly any scale

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• • Convenient the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats

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• • Easy storage aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails

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• • Compatible can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift

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• • Validated fully compatible with Thermo Scientific Pierce™ Cell Lysis Buffers

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nIncludes:nnFormulations with and without supplementary vial of EDTA for metalloprotease inhibition, both of which are offered in four package sizes.

Glucoside detergents are a class of non-ionic detergents commonly used in biochemical and biophysical research. These detergents are derived from glucose and possess unique properties that make them suitable for various applications. Glucoside detergents are known for their mildness, biocompatibility, and ability to solubilize and stabilize a wide range of biomolecules, including membrane proteins. Glucoside detergents are also compatible with many downstream applications, such as protein purification, crystallization, and structural studies.

Features of glucoside detergents
• Gentle on proteins, minimizing denaturation
• Non-ionic nature—reduces electrostatic interactions
• Low critical micelle concentration (CMC)
• High-purity with low UV absorptivity

Overall, glucoside detergents are versatile and can be used in various experimental techniques, including protein purification, crystallization, and structural biology studies. They are compatible with a wide range of biomolecules and can be employed in both membrane and soluble protein research. With their gentle nature and versatile performance, glucoside detergents have become valuable tools in the field of biochemical research.

Octyl-beta-Glucoside
Octyl-beta-Glucoside (OG) is a low molecular weight, non-ionic detergent with an octyl chain attached to the glucose moiety that has been widely used for membrane protein solubilization. OG is generally more soluble in aqueous solutions and forms clear and stable solutions at higher concentrations. OG typically forms larger micelles compared to OTG. The larger micelles of OG can be advantageous for certain applications, such as protein purification or structural studies, as they provide better solubility and reduced interference during downstream processes.

Properties of OG
• Alternative names: octyl-beta-glucopyranoside, octyl-beta-D-glucopyranoside
• Molecular weight: 292.37 g
• Micelle molecular weight: 8000 g
• Critical micelle concentration (CMC): 23 to 25 mM (0.6716 to 0.7300%, w/v)
• Aggregation number: 27
• Cloud point: >100°C
• Optically clear: low absorbance at 280 nm

Octylthio Glucoside
Octylthio Glucoside (OTG) is a low molecular weight, nonionic detergent that is effective for cell lysis and nondenaturing protein solubilization. It is resistant to beta-D-glucoside glucohydrolase degradation because the thioether group present in OTG provides protection against enzymatic hydrolysis. As a result, OTG can remain stable in the presence of beta-D-glucoside glucohydrolase enzymes, making it useful in applications where enzymatic stability is desired. However, it is important to note that the specific stability of OTG can still be influenced by factors such as enzyme concentration, reaction conditions, and the specific enzymes present.

Properties of OTG
• Chemical name: n-octyl-β-D-thioglucopyranoside
• Molecular weight: 44 g
• CMC: 9 mM (0.2772%, w/v)
• Cloud point: >100°C
• Optically clear: low absorbance at 280 nm

n-Nonyl-β-D-glucoside
n-Nonyl-β-D-glucoside (NG) has a longer alkyl chain (nonyl) compared to other glucosides. The longer alkyl chain in n-Nonyl-β-D-glucoside can confer higher hydrophobicity and potentially stronger detergent properties. This can enhance the solubilization of hydrophobic biomolecules and improve the extraction of membrane proteins. It may also induce different interactions with lipid bilayers, influencing the stability, fluidity, and permeability of the lipid bilayer, potentially impacting the behavior of membrane proteins. In addition, NG forms larger micelles compared to glucosides with shorter alkyl chains.

Properties of NG
• CMC: (H2O) ~ 6.5 mM (0.20%)
• Aggregation number: ~ 133
• Purity: ≥ 99% β+α (by HPLC analysis)
• pH: 5-8 (1% solution in water)

Thermo Scientific™ HisProbe-HRP is an nickel (Ni²+)-activated derivative of horseradish peroxidase (HRP). This product has been optimized for direct detection of recombinant histidine-tagged proteins and other histidine-rich proteins.

Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The detection kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC).

Highlights:

• Fast – His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol
• Economical – no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies
• High specificity – HisProbe-HRP can recognize his-tags independent of adjacent tags
• Less steric hindrance – HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies

Thermo Scientific™ Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of “heavy“ ¹³C- or ¹⁵N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

• Efficient – 100% label incorporation into proteins of living cells
• Flexible – different isotopes of heavy and/or light amino acids for arginine, lysine, leucine and proline enable the quantitation of peptides derived from MS-grade proteases
• Multiplex capabilities – several alternative isotopes of arginine and lysine are available that allow the analysis of multiple treatment conditions in each experiment
• High-quality supplements – heavy amino acids with >99% isotope purity

Get a gas-tight syringe for manual injections in your GC system. This syringe features a Luer-Tip connection and is available in a variety of sizes. Made of quality materials, they are designed for easy, accurate, precise and reproducible injections.

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter.

Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

Get a gas-tight syringe for manual injections in your GC system. This syringe features a Luer-Tip connection and is available in a variety of sizes. Made of quality materials, they are designed for easy, accurate, precise and reproducible injections.

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter.

Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

Thermo Scientific™ Pierce MBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short aromatic spacer arm (7.3 Ås).

Highlights:

• Popular for forming enzyme immunoconjugates
• Reactive groups: NHS-ester and maleimide
• Reactive toward: amino and sulfhydryl groups

Perform many small-volume applications with these safe diagnostic bottles that are leakproof when used with Nalgene closures. Ideal for use in test kits, sample storage and reagent shipping.

Perform many small-volume applications with Thermo Scientific™ Nalgene™ HDPE Diagnostic Bottles with Closure. These bottles are ideal for use in test kits, sample storage and shipping of small-volume, valuable reagents. They come packed in rigid trays for easy handling.

Save time and minimize contamination with gamma-sterilized Thermo Scientific™ Nalgene™ Rapid-Flow™ Filter Storage Bottles. Use with Nalgene Bottle-Top filters or as replacement/extra receptacles for filter units. Excellent as single-use storage containers.

• • Headspace allows for extra serum or other additives to be added to the filtrate

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• • Leakproof† screw caps to eliminate pH shift in bottle for longer life of stored media

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• • Sterilized by gamma radiation and individually bagged for a 5-year sterile shelf life

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• • Non-pyrogenic and non-cytotoxic

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• • Available with Fit for Purpose Sterile documentation

Increase filtration efficiency. Thermo Scientific™ Nalgene™ Rapid-Flow™ single use bottle tops feature the exclusive Rapid-Flow support column design, providing fast flow rates and high throughput. The last line of defense against cell culture contamination—Nalgene Rapid-Flow bottle tops are the best choice for the filtration of cell culture media, serum, additives and buffers.

• • Available in PES and SFCA membranes. Devices are color coded according to membrane.

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• • Devices feature the fastest flow rate and lowest clogging of comparable alternatives

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• • 0.1um PES available for mycoplasma protection

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• • 0.2um PES devices are stem cell and mouse embryonic assay (MEA) tested

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• • Non-pyrogenic and endotoxin free – Rapid-Flow filters meet the most rigorous testing standards for any vacuum filtration unit

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• • Available with Fit for Purpose Sterile documentation

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This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Prevent chemical accidents in the lab with these FEP Wash Bottles. Bottles labeled following the Globally Harmonized System (GHS).

Prevent chemical accidents in the lab with Nalgene™ Right-to-Understand FEP Wash Bottles. Bottles are permanently etched with chemical identification and safe handling information following the Globally Harmonized System (GHS) specifications as applicable to secondary laboratory containers. Wash bottles are ready to use when you transfer harsh laboratory chemicals from manufacturers’ containers. Six bottles available. For use with methyl ethyl ketone; n-hexane; toluene; xylene; methylene chloride; acetone.

Temporarily contain waste on the benchtop or under the lab hood using Thermo Scientific™ Nalgene™ Safety Waste Funnel Systems. Funnels feature the Easy Snap Safety Latch to minimize spillage if accidentally tipped over and a larger diameter funnel for easy pouring. The vent system helps reduce fumes and splashing while the removable neck screen traps large particles, like stir bars. Designed for solvent, chemical and biological waste, it is recommended for use with Nalgene containers.

Features:

• Polypropylene closure and funnel adaptor, polytetrafluoroethylene vent membrane
• Available in 10″ diameter funnel with 83B closure and 5.5″ funnel with 38-430 closure

Includes:

• HDPE funnel with attached lid
• Polypropylene closure/funnel adapter
• Screen insert
• Polypropylene/PTFE 0.45μm vent plug
• Filter

Thermo Scientific™ Nunc 15mL & 50mL Conical Sterile Polypropylene Centrifuge Tubes are premium, high-quality conical tubes that are environmentally friendly, offer the highest cleanliness with a recyclable plastic rack and allow for increased traceability with the largest writing area on the market. Nunc Labware Products are made from high-purity resins and molded using our state-of-the-art processes. Plastic labware is a safer alternative to glass without sacrificing accuracy.

• • Environmentally friendly recyclable plastic rack that reduces contamination from traditional foam packaging

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• • Leakproof to protect your samples

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Thermo Scientific™ Nunc 15mL & 50mL Conical Sterile Polypropylene Centrifuge Tubes are premium, high-quality conical tubes that are environmentally friendly, offer the highest cleanliness with a recyclable plastic rack and allow for increased traceability with the largest writing area on the market. Nunc Labware Products are made from high-purity resins and molded using our state-of-the-art processes. Plastic labware is a safer alternative to glass without sacrificing accuracy.

• • Environmentally friendly recyclable plastic rack that reduces contamination from traditional foam packaging

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• • Leakproof to protect your samples

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Thermo Scientific™ Nunc 15mL & 50mL Conical Sterile Polypropylene Centrifuge Tubes are premium, high-quality conical tubes that are environmentally friendly, offer the highest cleanliness with a recyclable plastic rack and allow for increased traceability with the largest writing area on the market. Nunc Labware Products are made from high-purity resins and molded using our state-of-the-art processes. Plastic labware is a safer alternative to glass without sacrificing accuracy.

• • Environmentally friendly recyclable plastic rack that reduces contamination from traditional foam packaging

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• • Leakproof to protect your samples

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Thermo Scientific™ Nunc 15mL & 50mL Conical Sterile Polypropylene Centrifuge Tubes are premium, high-quality conical tubes that are environmentally friendly, offer the highest cleanliness with a recyclable plastic rack and allow for increased traceability with the largest writing area on the market. Nunc Labware Products are made from high-purity resins and molded using our state-of-the-art processes. Plastic labware is a safer alternative to glass without sacrificing accuracy.

• • Environmentally friendly recyclable plastic rack that reduces contamination from traditional foam packaging

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• • Leakproof to protect your samples

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Store samples and perform agglutination assays with Thermo Scientific™ Nunc™ 96 DeepWell™ Polystyrene Plates, featuring transparent round bottom deep wells that hold up to 900µL per well.

• • Alphanumeric grid provides quick and easy sample identification.

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• • Optimized for robotics and liquid handling systems

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• • Round-bottom wells for optimal sample recovery

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• • Untreated surface

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• • Sealable with well caps and sealing tape

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• • Customized bar coding is available

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Store samples and perform agglutination assays with Thermo Scientific™ Nunc™ 96 DeepWell™ Polystyrene Plates, featuring transparent round bottom deep wells that hold up to 900µL per well.

• • Alphanumeric grid provides quick and easy sample identification.

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• • Optimized for robotics and liquid handling systems

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• • Round-bottom wells for optimal sample recovery

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• • Untreated surface

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• • Sealable with well caps and sealing tape

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• • Customized bar coding is available

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Increase storage capacity and improve mixing using Thermo Scientific™ Nunc™ 1.3 and 2.0mL DeepWell™ Plates with Shared-Wall Technology. Ideal for sample collection, storage, combinatorial chemistry and library applications, and resistant to most chemicals, solvents and alcohols used in combinatorial chemistry.

Features:

• • Ideal as a collection plate for Nunc Filter Plates

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• • Storage plate for compounds, samples or biomolecules

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• • Round-bottom wells reduce liquid retention

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• • Natural color

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• • Customized barcoding is available

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Working volumes:

• • 260251 and 260252 50-1000µL/well

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• • 278743 and 278752 50-1900 µL/well

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Recommended for: Sample collection; storage; combinatorial chemistry; library applications; bacterial and yeast growth; collection plates for Nunc filter plates

Perform adherent cell cultures in biotechnology, pharmaceutical and research laboratories, as well as in the production of vaccines and diagnostic kits with Thermo Scientific™ Nunc™ Cell Culture Tubes.

Features:

• Polystyrene tubes, polyethylene caps
• Flat-sided tube provides 5.5cm² culture area for grow of adherent cells
• Flat-sided tube allows easy microscopy; a coverslip can be used in the tube
• Medium retained in the flat-sided tube in horizontal position
• Treated with Thermo Scientific™ Nunclon™ Delta covering

Perform adherent cell cultures in biotechnology, pharmaceutical and research laboratories, as well as in the production of vaccines and diagnostic kits with Thermo Scientific™ Nunc™ Cell Culture Tubes.

Features:

• Polystyrene tubes, polyethylene caps
• Flat-sided tube provides 5.5cm² culture area for grow of adherent cells
• Flat-sided tube allows easy microscopy; a coverslip can be used in the tube
• Medium retained in the flat-sided tube in horizontal position
• Treated with Thermo Scientific™ Nunclon™ Delta covering

Non-treated Thermo Scientific™ Nunc™ Rectangular Dishes with ANSI™ standard dimensions are optimized for molecular biology applications using robotic instruments.

Rectangular Dishes 1, 4 and 8-Well

• • 1-well dish is ideal for HTS Petri dish applications

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• • 4- and 8-well dishes are ideal for assay compartmentalization, probe multiple gene sub-set

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• • 4-well dish holds four glass slides for storage

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n1-Well Format

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  • Convenient plate format for cell culture

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• • HTS Petri dish applications

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n4- and 8-Well Formatsn

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  • Assay compartmentalization

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• • Probe multiple gene subsets

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• • Wash Western blot strips

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• • 4-well dish holds four glass slides

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• • Useful in all areas of cell culture, including scale-up and cloning

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Ordering Information: Customized bar coding is available

Stringent quality specifications, and convenient features make Thermo Scientific™ Nunc™ Serological Pipettes a great choice for daily lab work. Bold, clear bi-directional graduations make it easy-to-read fluid volumes and dispensing amounts. Choose from a broad selection of sizes and packaging options.

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• • Wide Range of Volumes and Packaging Option

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• • Color-coded pipettes and packaging for convenient volume selection

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• • Individually packed pipettes in either paper/plastic or plastic peel for high sterility assurance

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• • Reduce packaging waste with bulk packed options

• • Ensures dispensing accuracy +/-2% for 1mL and 2 mL and +/-1% for 5 mL, 10 mL, 25 mL, 50 mL at full volume

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• • High contrast graduations for easy-to-read volumes

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• • Reverse graduations aid in determining dispensing volume

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• • Increase pipette capacity, and accommodates additional volumes

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• • Prevent aerosol or liquid contamination of the pipetting device while further reducing sample-to-sample contamination risks

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Specifications:

• • Material: Ultra clear, 100% virgin polystyrene for maximum clarity

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• • Contaminant Free: RNase/DNase free, Human DNA free, Non-Cytotoxic, Non-Pyrogenic

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• • Sterility: E-Beam irradiation to SAL of 10^-6

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• • Shelf Life: 5 years from Manufacturing Date

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• • Certificate of Quality

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Stringent quality specifications, and convenient features make Thermo Scientific™ Nunc™ Serological Pipettes a great choice for daily lab work. Bold, clear bi-directional graduations make it easy-to-read fluid volumes and dispensing amounts. Choose from a broad selection of sizes and packaging options.

• • Wide Range of Volumes and Packaging Option

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• • Color-coded pipettes and packaging for convenient volume selection

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• • Individually packed pipettes in either paper/plastic or plastic peel for high sterility assurance

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• • Reduce packaging waste with bulk packed options

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• • Ensures dispensing accuracy +/-2% for 1mL and 2 mL and +/-1% for 5 mL, 10 mL, 25 mL, 50 mL at full volume

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• • High contrast graduations for easy-to-read volumes

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• • Reverse graduations aid in determining dispensing volume

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• • Increase pipette capacity, and accommodates additional volumes

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• • Prevent aerosol or liquid contamination of the pipetting device while further reducing sample-to-sample contamination risks

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Specifications:

• • Material: Ultra clear, 100% virgin polystyrene for maximum clarity

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• • Contaminant Free: RNase/DNase free, Human DNA free, Non-Cytotoxic, Non-Pyrogenic

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• • Sterility: E-Beam irradiation to SAL of 10^-6

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• • Shelf Life: 5 years from Manufacturing Date

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Product Testing 

• • Certificate of Quality

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Perform quick protein runs with the economical and simple, yet rugged Thermo Scientific™ Owl™ P81 Single-Sided Vertical Electrophoresis System.

Features:

• Upper buffer chamber extends the length of the gel to distribute heat evenly across the entire gel surface
• Side clamps press glass plates against silicone gasket to provide uniform pressure and form a leakproof seal resulting in flat, even banding patterns
• Notched alumina ceramic plate (included) may be used in front of notched glass for cooler, faster runs

Includes:

• Upper and lower buffer chambers
• Thermo Scientific™ SuperSafe™ lid with attached power supply leads
• Blank glass plate
• Notched glass plate
• Notched alumina plate
• Two combs (10 well, 0.8mm thick)
• Spacer set (0.8mm thick)

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. A blue chromophore is bound to all proteins, except proteins of two reference bands of 70kDa and 25kDa that are colored with an orange dye and one green reference band of 10kDa. PageRuler Plus Prestained Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required before loading onto a gel.nnHighlights:n

• • Size range – nine proteins spanning 10 to 250kDa

• • Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil

• • Sharp bands – color-coded bands of similar intesity for easy visualization

• • Quality tested – each lot evaluated by SDS-PAGE and Western blotting

• • Bright reference bands – orange at 70 and 25kDa, and green at 10kDa

• • Membrane-compatible – colored bands transfer to membranes for Western blotting

Includes:nnDye-stained proteins in 62.5mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN₃ and 33% glycerol.

Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.

nBenefits of using Pierce Concentrated Buffer stock solutions include:
• Convenient—no weighing, no pH adjustment, no need to stock individual components
• Improved accuracy—ready-to-use formula, easily diluted without weighing chemical components
• Consistent quality—our quality control ensures that every prep will yield the same, consistent buffer

20X PBS Tween-20 can be used to prepare PBS-Tween (PBS-T) wash buffers for ELISA, western, and other immunoassays, as well as blocking buffer for plate-based assays. When diluted to 1X with water, 20X PBS Tween-20 makes 10 mM sodium phosphate, 0.15 M NaCl, 0.05% Tween™ 20 buffer at pH 7.5.

20X TBS Tween-20 (Tris-buffered saline with Tween™ 20 detergent) can be used to prepare TBS-Tween (TBS-T) wash buffers or blocking buffer diluent for applications such as ELISA, western blotting, and other immunoassays. When diluted 20-fold in water, a TBS Tween-20 buffer containing 25 mM Tris, 0.15 M NaCl, 0.05% Tween-20 at pH 7.5 is ready for use.

20X Tris-Buffered Saline (TBS) is a stock solution for preparing Tris-NaCl buffer for use as a wash buffer and antibody diluent for ELISA, western blotting, and other immunoassays. 20X Tris Buffered Saline (TBS) makes 25 mM Tris, 0.15 M NaCl with pH 7.2 to 7.5 when diluted to 1X with water.

20X Phosphate-Buffered Saline (PBS) is ideal for preparing PBS buffers for use in crosslinking, biotinylation, and fluorescent labeling reactions requiring an amine-free buffer. When diluted 20-fold in water, the solution yields 10 mM sodium phosphate, 0.15 M NaCl at pH 7.5.

10X Tris-Glycine-SDS Buffer makes 0.025 M Tris, 0.192M glycine, 0.1% sodium dodecyl sulfate (SDS) at pH 8.5 when diluted to 1X with water.

20X Borate Buffer is ideal for preparing sodium borate buffer solutions for use in protein modification procedures requiring amine-free buffer at an alkaline pH. 20X Borate Buffer makes 50 mM borate at pH 8.5 when diluted to 1X with water.

20X Modified Dulbecco’s PBS is used for preparing physiological Na- and K-phosphate buffered saline (D-PBS) used for wash buffers and antibody diluents in immunoassays like ELISA and western blot. 20X Modified Dulbecco’s PBS makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, pH 7.4 when diluted to 1X with water.

10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.5) transfer buffer used for western blotting using Tris-glycine gel electrophoresis. When diluted 10-fold in water or 20% methanol, the solution yields 0.025 M Tris, 0.192 M glycine at pH 8.5.

10X TBE Buffer stock solution is ideal for casting and preparing Tris-borate-EDTA (TBE) running buffer used for agarose and polyacrylamide gel electrophoresis of nucleic acids. When diluted to 1X, the TBE Buffer contains 0.089 M Tris, 0.089 M borate, 2 mM EDTA with pH 8.2 to 8.4.

20X Modified Dulbecco’s PBS Tween-20 will make physiological sodium- and potassium-phosphate buffered saline (D-PBS) to be used as a wash buffer for ELISA, western, and other immunoassays or as diluent or blocking buffer for plate-based assays. 20X Modified Dulbecco’s PBS Tween-20 (D-PBS-T) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, 0.05% Tween™ 20 at pH 7.4 when diluted to 1X with water.

20X TAE Buffer is a space-saving stock solution that is ideal for casting and preparing Tris-acetate EDTA (TAE) running buffer for agarose gel electrophoresis of nucleic acids. When diluted to 1X with water, a 0.04 M Tris, 0.04 M acetate, 1 mM EDTA buffer at pH 8.2 to 8.4 is ready for use.

Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.

nBenefits of using Pierce Concentrated Buffer stock solutions include:
• Convenient—no weighing, no pH adjustment, no need to stock individual components
• Improved accuracy—ready-to-use formula, easily diluted without weighing chemical components
• Consistent quality—our quality control ensures that every prep will yield the same, consistent buffer

20X PBS Tween-20 can be used to prepare PBS-Tween (PBS-T) wash buffers for ELISA, western, and other immunoassays, as well as blocking buffer for plate-based assays. When diluted to 1X with water, 20X PBS Tween-20 makes 10 mM sodium phosphate, 0.15 M NaCl, 0.05% Tween™ 20 buffer at pH 7.5.

20X TBS Tween-20 (Tris-buffered saline with Tween™ 20 detergent) can be used to prepare TBS-Tween (TBS-T) wash buffers or blocking buffer diluent for applications such as ELISA, western blotting, and other immunoassays. When diluted 20-fold in water, a TBS Tween-20 buffer containing 25 mM Tris, 0.15 M NaCl, 0.05% Tween-20 at pH 7.5 is ready for use.

20X Tris-Buffered Saline (TBS) is a stock solution for preparing Tris-NaCl buffer for use as a wash buffer and antibody diluent for ELISA, western blotting, and other immunoassays. 20X Tris Buffered Saline (TBS) makes 25 mM Tris, 0.15 M NaCl with pH 7.2 to 7.5 when diluted to 1X with water.

20X Phosphate-Buffered Saline (PBS) is ideal for preparing PBS buffers for use in crosslinking, biotinylation, and fluorescent labeling reactions requiring an amine-free buffer. When diluted 20-fold in water, the solution yields 10 mM sodium phosphate, 0.15 M NaCl at pH 7.5.

10X Tris-Glycine-SDS Buffer makes 0.025 M Tris, 0.192M glycine, 0.1% sodium dodecyl sulfate (SDS) at pH 8.5 when diluted to 1X with water.

20X Borate Buffer is ideal for preparing sodium borate buffer solutions for use in protein modification procedures requiring amine-free buffer at an alkaline pH. 20X Borate Buffer makes 50 mM borate at pH 8.5 when diluted to 1X with water.

20X Modified Dulbecco’s PBS is used for preparing physiological Na- and K-phosphate buffered saline (D-PBS) used for wash buffers and antibody diluents in immunoassays like ELISA and western blot. 20X Modified Dulbecco’s PBS makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, pH 7.4 when diluted to 1X with water.

10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.5) transfer buffer used for western blotting using Tris-glycine gel electrophoresis. When diluted 10-fold in water or 20% methanol, the solution yields 0.025 M Tris, 0.192 M glycine at pH 8.5.

10X TBE Buffer stock solution is ideal for casting and preparing Tris-borate-EDTA (TBE) running buffer used for agarose and polyacrylamide gel electrophoresis of nucleic acids. When diluted to 1X, the TBE Buffer contains 0.089 M Tris, 0.089 M borate, 2 mM EDTA with pH 8.2 to 8.4.

20X Modified Dulbecco’s PBS Tween-20 will make physiological sodium- and potassium-phosphate buffered saline (D-PBS) to be used as a wash buffer for ELISA, western, and other immunoassays or as diluent or blocking buffer for plate-based assays. 20X Modified Dulbecco’s PBS Tween-20 (D-PBS-T) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, 0.05% Tween™ 20 at pH 7.4 when diluted to 1X with water.

20X TAE Buffer is a space-saving stock solution that is ideal for casting and preparing Tris-acetate EDTA (TAE) running buffer for agarose gel electrophoresis of nucleic acids. When diluted to 1X with water, a 0.04 M Tris, 0.04 M acetate, 1 mM EDTA buffer at pH 8.2 to 8.4 is ready for use.

Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.

nBenefits of using Pierce Concentrated Buffer stock solutions include:
• Convenient—no weighing, no pH adjustment, no need to stock individual components
• Improved accuracy—ready-to-use formula, easily diluted without weighing chemical components
• Consistent quality—our quality control ensures that every prep will yield the same, consistent buffer

20X PBS Tween-20 can be used to prepare PBS-Tween (PBS-T) wash buffers for ELISA, western, and other immunoassays, as well as blocking buffer for plate-based assays. When diluted to 1X with water, 20X PBS Tween-20 makes 10 mM sodium phosphate, 0.15 M NaCl, 0.05% Tween™ 20 buffer at pH 7.5.

20X TBS Tween-20 (Tris-buffered saline with Tween™ 20 detergent) can be used to prepare TBS-Tween (TBS-T) wash buffers or blocking buffer diluent for applications such as ELISA, western blotting, and other immunoassays. When diluted 20-fold in water, a TBS Tween-20 buffer containing 25 mM Tris, 0.15 M NaCl, 0.05% Tween-20 at pH 7.5 is ready for use.

20X Tris-Buffered Saline (TBS) is a stock solution for preparing Tris-NaCl buffer for use as a wash buffer and antibody diluent for ELISA, western blotting, and other immunoassays. 20X Tris Buffered Saline (TBS) makes 25 mM Tris, 0.15 M NaCl with pH 7.2 to 7.5 when diluted to 1X with water.

20X Phosphate-Buffered Saline (PBS) is ideal for preparing PBS buffers for use in crosslinking, biotinylation, and fluorescent labeling reactions requiring an amine-free buffer. When diluted 20-fold in water, the solution yields 10 mM sodium phosphate, 0.15 M NaCl at pH 7.5.

10X Tris-Glycine-SDS Buffer makes 0.025 M Tris, 0.192M glycine, 0.1% sodium dodecyl sulfate (SDS) at pH 8.5 when diluted to 1X with water.

20X Borate Buffer is ideal for preparing sodium borate buffer solutions for use in protein modification procedures requiring amine-free buffer at an alkaline pH. 20X Borate Buffer makes 50 mM borate at pH 8.5 when diluted to 1X with water.

20X Modified Dulbecco’s PBS is used for preparing physiological Na- and K-phosphate buffered saline (D-PBS) used for wash buffers and antibody diluents in immunoassays like ELISA and western blot. 20X Modified Dulbecco’s PBS makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, pH 7.4 when diluted to 1X with water.

10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.5) transfer buffer used for western blotting using Tris-glycine gel electrophoresis. When diluted 10-fold in water or 20% methanol, the solution yields 0.025 M Tris, 0.192 M glycine at pH 8.5.

10X TBE Buffer stock solution is ideal for casting and preparing Tris-borate-EDTA (TBE) running buffer used for agarose and polyacrylamide gel electrophoresis of nucleic acids. When diluted to 1X, the TBE Buffer contains 0.089 M Tris, 0.089 M borate, 2 mM EDTA with pH 8.2 to 8.4.

20X Modified Dulbecco’s PBS Tween-20 will make physiological sodium- and potassium-phosphate buffered saline (D-PBS) to be used as a wash buffer for ELISA, western, and other immunoassays or as diluent or blocking buffer for plate-based assays. 20X Modified Dulbecco’s PBS Tween-20 (D-PBS-T) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, 0.05% Tween™ 20 at pH 7.4 when diluted to 1X with water.

20X TAE Buffer is a space-saving stock solution that is ideal for casting and preparing Tris-acetate EDTA (TAE) running buffer for agarose gel electrophoresis of nucleic acids. When diluted to 1X with water, a 0.04 M Tris, 0.04 M acetate, 1 mM EDTA buffer at pH 8.2 to 8.4 is ready for use.

Thermo Scientific™ Pierce BCA Protein Assay Kit remains one of the most popular protein quantitation methods worldwide.

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n

• • Colorimetric – estimate visually or measure with a standard spectrophotometer or plate reader (562nm)

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• • Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods

n

• • Compatible – unaffected by typical concentrations of most ionic and nonionic detergents

n

• • Moderately fast – much easier and four times faster than the classical Lowry method

n

• • High linearity – linear working range for BSA equals 20 to 2000µg/mL

n

• • Sensitive – detect down to 5µg/mL with the enhanced protocol

n

• • Studying protein:protein interactions

n

• • Measuring column fractions after affinity chromatography

n

• • Estimating percent recovery of membrane proteins from cell extracts

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• • High-throughput screening of fusion proteins

Thermo Scientific™ Pierce Biotinylated Protein Interaction Pull-Down Kit contains the necessary components to capture and purify interactors of a biotin-labeled protein or ligand.

The kit makes it possible to perform an efficient pull-down assay with high protein recovery and reproducibility. This protocol uses immobilized streptavidin to capture a biotinylated protein and a spin column format that enables complete gel retention during each capture, wash and elution step. When the biotinylated bait protein is immobilized, it can withstand stringent washes and remains bound during prey protein elution, which results in an isolated prey protein without bait protein contamination. This technique is ideal when using native or recombinant proteins that are not expressed with a fusion tag. Additionally, prey protein is isolated from the immobilized bait protein using native binding conditions and nondenaturing elution conditions. The Biotinylated Protein Interaction Pull-Down Kit is designed to teach the method to first-time users and to increase ease-of-use and convenience for experienced researchers.

Highlights:

• Biotin pull-down – purifies protein interactors of any biotinylated protein or biotin-tagged ligand
• Complete kit – provides all components and detailed protocol for purifying protein:protein interactions
• No special equipment needed – uses common laboratory equipment and reagents (e.g., microcentrifuge)
• Spin column efficiency – microcentrifuge spin columns facilitate microcentrifuge spin columns facilitate simple and efficient manipulation of streptavidin agarose beads, including simple processing of multiple samples

Includes:

Streptavidin agarose resin, TBS pack, biotin blocking buffer, wash buffer, elution buffer, spin columns and collection tubes

Recommended for:

Discover a new protein interaction complex using a biotinylated protein as the bait to capture a putative binding partner from a cell lysate or other sample; Confirm expression of a known protein interaction target in a cell lysate; Determine the ability of two purified proteins to interact (bind) in various buffer conditions; Extract protein:protein interaction information from in vitro transcription/ translation lysates

Perform ribonucleotide and oligonucleotide RNA isolation with Thermo Scientific™ Pierce Boronic Acid Resin.

This immobilized boronic acid is an easy-to-use polyacrylamide affinity support for purification of ribonucleotides and other small molecular weight compounds that contain cis-diol groups. The ligand (m-aminophenyl-boronic acid) binds to the cis-diol groups on the sugar portion of nucleotides, forming a reversible five-member ring complex. After washing away non-bound molecules from the sample, the complex can be dissociated and the nucleotide eluted by lowering the pH or by addition of sorbitol.

Highlights:

• Ligand – boronic acid
• Support – beaded polyacrylamide (1800 MW exclusion limit)
• Spacer – m-aminophenyl group
• Loading – 100μmol boronate per milliliter of settled resin
• Capacity – at least 99% binding and recovery of 110μmol AMP per milliliter of settled resin
• Format – 50% resin slurry in 0.05% sodium azide

Measure protein concentration using a stable formulation of the original Bradford reagent described in the literature with Thermo Scientific™ Pierce Coomassie Protein Assay Kit.

The Pierce Coomassie Protein Assay Kit is a ready-to-use formulation of the popular assay reagent originally described by Bradford in 1976. When mixed with a protein solution, the acidic Coomassie-dye reagent changes color from brown to blue in proportion to the amount of protein present in the sample. Protein determinations are made by comparison to the color response of protein assay standards, usually prepared as a series of known dilutions of bovine serum albumin (BSA) or bovine gamma globulin (BGG). The kit includes Coomassie Protein Assay Reagent and a package of Albumin Standard Ampules. The simple procedure is adaptable to nearly any volume scale, including test tubes, cuvets and microplates.

Highlights:

• Bradford reagent – stable, ready-to-use kit of the classical Bradford assay reagent
• Colorimetric – measure with a standard spectrophotometer or plate reader (595nm)
• Easy to use – single reagent; no working reagent preparation required
• Fast – almost immediate color development; add, mix and read results
• Broad range – detects protein concentration in the range 1 to 1500μg/mL
• Flexible – microplate and cuvet protocols provided with the instructions and adaptable to several target working ranges

Includes:

Coomassie Protein Assay Reagent and 10 ampules of Albumin Standard

Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometry (MS) provide high sensitivity and mass accuracy; however, interfering molecules from the sample can result in reduced sensitivity and poor-quality spectra. Sample clean-up with C18 resins significantly improves signal-to-noise ratios and sequence coverage by removing MS-incompatible salts and detergents commonly used in protein or peptide preparation. The optimized protocol for the Pierce C-18 Spin Columns effectively removes contaminants and elutes peptides in MS-compatible solutions.

Pierce C18 Spin Columns
Thermo Scientific Pierce C18 Spin Columns are ready-to-use centrifuge columns of porous C18 reverse-phase resin for peptide sample preparation for MS and other analysis methods. The protocol has been optimized to efficiently remove interfering contaminants and release peptides in MS-compatible solutions. Columns can be used effectively for processing peptides derived from 10 ng to 30 μg of protein. Sensitivity and detection limits are dependent on the downstream application.

• Efficient—effectively removes interfering contaminants to improve spectral quality and is suitable for a variety of reverse-phase-compatible contaminants
• Flexible—works with a wide variety of load volumes and concentrations; no need to reduce sample volume before application
• Convenient—spin column format requires no special equipment to process multiple samples simultaneously
• Robust—special C18 resin allows excellent recovery percentages, even at low (sub-picomole) sample loads

Pierce C18 Spin Tips
Thermo Scientific Pierce C18 Spin Tips enable fast and efficient capture, concentration, desalting, and elution of up to 10 μg of peptides per 20 μL of sample with volumes ranging from 10 to 100 μL. The five-step procedure is simple and requires less than five minutes to process protein digests, strong cation exchange fractions, and other protein and peptide samples for mass spectrometric (MS) analyses. The Pierce C18 Spin Tips offer excellent flow properties with a high-efficiency monolithic C18 sorbent for fast wetting, loading, washing, and eluting.

• Efficient—effectively removes interfering contaminants to improve spectral quality and is suitable for a variety of reverse-phase-compatible contaminants
• Rapid—C18 fast-flow tips have low resistance and improved flow
• Convenient—spin tips are provided with tip adaptor for easy centrifugation
• Cleaner sample—device filters particulates that can cause autosampler and column clogging

Applications
• Clean-up of peptides after protein digestion
• Peptide sample concentration and desalting
• Sample preparation for MALDI peptide analysis and proteomics workflows

Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometry (MS) are essential for studying biological compounds because of the high sensitivity and mass accuracy they provide. However, many of the buffers and compounds common to biological samples interfere with both MALDI-MS and ESI-MS, resulting in reduced sensitivity and poor-quality spectra. Pierce C-18 Spin Columns remove interfering contaminants and release peptides in MS-compatible solutions, providing an effective tool that aids in the MS examination of post-translational modifications or for the identification of proteins by peptide mapping.

Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometry (MS) provide high sensitivity and mass accuracy; however, interfering molecules from the sample can result in reduced sensitivity and poor-quality spectra. Sample clean-up with C18 resins significantly improves signal-to-noise ratios and sequence coverage by removing MS-incompatible salts and detergents commonly used in protein or peptide preparation. The optimized protocol for the Pierce C-18 Spin Columns effectively removes contaminants and elutes peptides in MS-compatible solutions.

Pierce C18 Spin Columns
Thermo Scientific Pierce C18 Spin Columns are ready-to-use centrifuge columns of porous C18 reverse-phase resin for peptide sample preparation for MS and other analysis methods. The protocol has been optimized to efficiently remove interfering contaminants and release peptides in MS-compatible solutions. Columns can be used effectively for processing peptides derived from 10 ng to 30 μg of protein. Sensitivity and detection limits are dependent on the downstream application.

• Efficient—effectively removes interfering contaminants to improve spectral quality and is suitable for a variety of reverse-phase-compatible contaminants
• Flexible—works with a wide variety of load volumes and concentrations; no need to reduce sample volume before application
• Convenient—spin column format requires no special equipment to process multiple samples simultaneously
• Robust—special C18 resin allows excellent recovery percentages, even at low (sub-picomole) sample loads

Pierce C18 Spin Tips
Thermo Scientific Pierce C18 Spin Tips enable fast and efficient capture, concentration, desalting, and elution of up to 10 μg of peptides per 20 μL of sample with volumes ranging from 10 to 100 μL. The five-step procedure is simple and requires less than five minutes to process protein digests, strong cation exchange fractions, and other protein and peptide samples for mass spectrometric (MS) analyses. The Pierce C18 Spin Tips offer excellent flow properties with a high-efficiency monolithic C18 sorbent for fast wetting, loading, washing, and eluting.

• Efficient—effectively removes interfering contaminants to improve spectral quality and is suitable for a variety of reverse-phase-compatible contaminants
• Rapid—C18 fast-flow tips have low resistance and improved flow
• Convenient—spin tips are provided with tip adaptor for easy centrifugation
• Cleaner sample—device filters particulates that can cause autosampler and column clogging

Applications
• Clean-up of peptides after protein digestion
• Peptide sample concentration and desalting
• Sample preparation for MALDI peptide analysis and proteomics workflows

Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometry (MS) are essential for studying biological compounds because of the high sensitivity and mass accuracy they provide. However, many of the buffers and compounds common to biological samples interfere with both MALDI-MS and ESI-MS, resulting in reduced sensitivity and poor-quality spectra. Pierce C-18 Spin Columns remove interfering contaminants and release peptides in MS-compatible solutions, providing an effective tool that aids in the MS examination of post-translational modifications or for the identification of proteins by peptide mapping.

Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.

Benefits of using Pierce Concentrated Buffer stock solutions include:
• Convenient—no weighing, no pH adjustment, no need to stock individual components
• Improved accuracy—ready-to-use formula, easily diluted without weighing chemical components
• Consistent quality—our quality control ensures that every prep will yield the same, consistent buffer

20X PBS Tween-20 can be used to prepare PBS-Tween (PBS-T) wash buffers for ELISA, western, and other immunoassays, as well as blocking buffer for plate-based assays. When diluted to 1X with water, 20X PBS Tween-20 makes 10 mM sodium phosphate, 0.15 M NaCl, 0.05% Tween™ 20 buffer at pH 7.5.

20X TBS Tween-20 (Tris-buffered saline with Tween™ 20 detergent) can be used to prepare TBS-Tween (TBS-T) wash buffers or blocking buffer diluent for applications such as ELISA, western blotting, and other immunoassays. When diluted 20-fold in water, a TBS Tween-20 buffer containing 25 mM Tris, 0.15 M NaCl, 0.05% Tween-20 at pH 7.5 is ready for use.

20X Tris-Buffered Saline (TBS) is a stock solution for preparing Tris-NaCl buffer for use as a wash buffer and antibody diluent for ELISA, western blotting, and other immunoassays. 20X Tris Buffered Saline (TBS) makes 25 mM Tris, 0.15 M NaCl with pH 7.2 to 7.5 when diluted to 1X with water.

20X Phosphate-Buffered Saline (PBS) is ideal for preparing PBS buffers for use in crosslinking, biotinylation, and fluorescent labeling reactions requiring an amine-free buffer. When diluted 20-fold in water, the solution yields 10 mM sodium phosphate, 0.15 M NaCl at pH 7.5.

10X Tris-Glycine-SDS Buffer makes 0.025 M Tris, 0.192M glycine, 0.1% sodium dodecyl sulfate (SDS) at pH 8.5 when diluted to 1X with water.

20X Borate Buffer is ideal for preparing sodium borate buffer solutions for use in protein modification procedures requiring amine-free buffer at an alkaline pH. 20X Borate Buffer makes 50 mM borate at pH 8.8 when diluted to 1X with water.

20X Modified Dulbecco’s PBS is used for preparing physiological Na- and K-phosphate buffered saline (D-PBS) used for wash buffers and antibody diluents in immunoassays like ELISA and western blot. 20X Modified Dulbecco’s PBS makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, pH 7.4 when diluted to 1X with water.

10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.5) transfer buffer used for western blotting using Tris-glycine gel electrophoresis. When diluted 10-fold in water or 20% methanol, the solution yields 0.025 M Tris, 0.192 M glycine at pH 8.5.

10X TBE Buffer stock solution is ideal for casting and preparing Tris-borate-EDTA (TBE) running buffer used for agarose and polyacrylamide gel electrophoresis of nucleic acids. When diluted to 1X, the TBE Buffer contains 0.089 M Tris, 0.089 M borate, 2 mM EDTA with pH 8.2 to 8.4.

20X Modified Dulbecco’s PBS Tween-20 will make physiological sodium- and potassium-phosphate buffered saline (D-PBS) to be used as a wash buffer for ELISA, western, and other immunoassays or as diluent or blocking buffer for plate-based assays. 20X Modified Dulbecco’s PBS Tween-20 (D-PBS-T) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, 0.05% Tween™ 20 at pH 7.4 when diluted to 1X with water.

20X TAE Buffer is a space-saving stock solution that is ideal for casting and preparing Tris-acetate EDTA (TAE) running buffer for agarose gel electrophoresis of nucleic acids. When diluted to 1X with water, a 0.04 M Tris, 0.04 M acetate, 1 mM EDTA buffer at pH 8.2 to 8.4 is ready for use.

Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.

Benefits of using Pierce Concentrated Buffer stock solutions include:
• Convenient—no weighing, no pH adjustment, no need to stock individual components
• Improved accuracy—ready-to-use formula, easily diluted without weighing chemical components
• Consistent quality—our quality control ensures that every prep will yield the same, consistent buffer

20X PBS Tween-20 can be used to prepare PBS-Tween (PBS-T) wash buffers for ELISA, western, and other immunoassays, as well as blocking buffer for plate-based assays. When diluted to 1X with water, 20X PBS Tween-20 makes 10 mM sodium phosphate, 0.15 M NaCl, 0.05% Tween™ 20 buffer at pH 7.5.

20X TBS Tween-20 (Tris-buffered saline with Tween™ 20 detergent) can be used to prepare TBS-Tween (TBS-T) wash buffers or blocking buffer diluent for applications such as ELISA, western blotting, and other immunoassays. When diluted 20-fold in water, a TBS Tween-20 buffer containing 25 mM Tris, 0.15 M NaCl, 0.05% Tween-20 at pH 7.5 is ready for use.

20X Tris-Buffered Saline (TBS) is a stock solution for preparing Tris-NaCl buffer for use as a wash buffer and antibody diluent for ELISA, western blotting, and other immunoassays. 20X Tris Buffered Saline (TBS) makes 25 mM Tris, 0.15 M NaCl with pH 7.2 to 7.5 when diluted to 1X with water.

20X Phosphate-Buffered Saline (PBS) is ideal for preparing PBS buffers for use in crosslinking, biotinylation, and fluorescent labeling reactions requiring an amine-free buffer. When diluted 20-fold in water, the solution yields 10 mM sodium phosphate, 0.15 M NaCl at pH 7.5.

10X Tris-Glycine-SDS Buffer makes 0.025 M Tris, 0.192M glycine, 0.1% sodium dodecyl sulfate (SDS) at pH 8.5 when diluted to 1X with water.

20X Borate Buffer is ideal for preparing sodium borate buffer solutions for use in protein modification procedures requiring amine-free buffer at an alkaline pH. 20X Borate Buffer makes 50 mM borate at pH 8.8 when diluted to 1X with water.

20X Modified Dulbecco’s PBS is used for preparing physiological Na- and K-phosphate buffered saline (D-PBS) used for wash buffers and antibody diluents in immunoassays like ELISA and western blot. 20X Modified Dulbecco’s PBS makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, pH 7.4 when diluted to 1X with water.

10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.5) transfer buffer used for western blotting using Tris-glycine gel electrophoresis. When diluted 10-fold in water or 20% methanol, the solution yields 0.025 M Tris, 0.192 M glycine at pH 8.5.

10X TBE Buffer stock solution is ideal for casting and preparing Tris-borate-EDTA (TBE) running buffer used for agarose and polyacrylamide gel electrophoresis of nucleic acids. When diluted to 1X, the TBE Buffer contains 0.089 M Tris, 0.089 M borate, 2 mM EDTA with pH 8.2 to 8.4.

20X Modified Dulbecco’s PBS Tween-20 will make physiological sodium- and potassium-phosphate buffered saline (D-PBS) to be used as a wash buffer for ELISA, western, and other immunoassays or as diluent or blocking buffer for plate-based assays. 20X Modified Dulbecco’s PBS Tween-20 (D-PBS-T) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, 0.05% Tween™ 20 at pH 7.4 when diluted to 1X with water.

20X TAE Buffer is a space-saving stock solution that is ideal for casting and preparing Tris-acetate EDTA (TAE) running buffer for agarose gel electrophoresis of nucleic acids. When diluted to 1X with water, a 0.04 M Tris, 0.04 M acetate, 1 mM EDTA buffer at pH 8.2 to 8.4 is ready for use.

Thermo Scientific Pierce Coomassie Brilliant Blue dyes are composed of one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains. Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye.

Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye.

Typically, coomassie gel stains and protein assay reagents are formulated as very acidic solutions in 25 to 50% methanol. In acidic conditions, the dye binds to proteins primarily through basic amino acids (primarily arginine, lysine, and histidine), and the number of coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Protein binding causes the dye to change from reddish-brown to bright blue (absorption maximum equals 595 nm).

Features include:
• Easy detection—develops intensely colored complexes with proteins
• High sensitivity—can determine as little as 0.5 μg/cm2 of protein present in a gel matrix
• Reversible staining—anion of Coomassie Brilliant Blue dye formed in the acidic staining medium combines with the protonated amino groups of proteins by electrostatic interaction; resulting complex is reversible under proper conditions
• Differentiation between bound and unbound dye—when dissolved in 0.01 M citrate buffer at pH 3.0, has an absorption maximum at 555 nm; protein-dye complex is characterized by a peak slightly broader than that of free dye with a maximum at 549 nm

Thermo Scientific Pierce Dimethylformamide (DMF) is a sequencing-grade preparation with high purity and consistent absorptivity properties for use in HPLC and mass spectrometry methods.

This sequencing-grade dimethylformamide is specially purified by a proprietary method and tested to ensure lot-to-lot consistency with a low UV absorbance to provide you with the most sensitive detection across all wavelengths. DMF is packaged in solvent-rinsed amber glass bottles and sealed with PTFE-lined fluorocarbon caps for ultimate protection.

Highlights:

• Highly purified and tested to ensure reliable performance in HPLC, mass spectrometry and sequencing applications
• Packaged in solvent-rinsed amber glass bottles and sealed with PTFE-lined fluorocarbon caps

Product Details:

• General properties of DMF
• Alternative names: n,n-Dimethylformamide, n-Formyldimethylamine
• Molecular formula: C₃H₇ON
• Molecular weight: 73.09
• Density:0.945g/mL
• CAS number: 68-12-2

Pierce sequencing-grade DMF is specially purified and tested to the highest specifications to ensure the integrity of your data, maximize sensitivity in your assay and prolong the life of your equipment.

Thermo Scientific™ Pierce Disposable Plastic Columns are empty columns with matching frits and caps for use in a variety of resin-based affinity or desalting techniques.

These bulk packages of empty columns are economical and convenient devices for packing affinity purification supports and desalting resins for gravity-flow procedures. Three columns sizes are offered, suitable for procedures requiring 0.5 to 10mL resin-bed volumes. The plastic columns are supplied with matching pairs of polyethylene discs and polypropylene caps (top and bottom). The columns are economically-priced for single use and sufficiently durable to prepare and store columns for multiple uses.

Highlights:

• For bench top gravity-flow affinity purification and size exclusion chromatography
• Supplied complete with porous polyethylene discs, stoppers and end caps
• Columns can be pre-packed and stored until needed
• Compatible with most types of aqueous buffer eluents commonly used in chromatography
• Plastic funnels can be attached to expand the buffer reservoir for increased flow rates

Includes:

Trial pack contains two columns of each available size (six columns total), matching column accessories (discs and caps), and one funnel (column reservoir extender)

Thermo Scientific™ Pierce Disposable Plastic Columns are empty columns with matching frits and caps for use in a variety of resin-based affinity or desalting techniques.

These bulk packages of empty columns are economical and convenient devices for packing affinity purification supports and desalting resins for gravity-flow procedures. Three columns sizes are offered, suitable for procedures requiring 0.5 to 10mL resin-bed volumes. The plastic columns are supplied with matching pairs of polyethylene discs and polypropylene caps (top and bottom). The columns are economically-priced for single use and sufficiently durable to prepare and store columns for multiple uses.

Highlights:

• For bench top gravity-flow affinity purification and size exclusion chromatography
• Supplied complete with porous polyethylene discs, stoppers and end caps
• Columns can be pre-packed and stored until needed
• Compatible with most types of aqueous buffer eluents commonly used in chromatography
• Plastic funnels can be attached to expand the buffer reservoir for increased flow rates

Includes:

Trial pack contains two columns of each available size (six columns total), matching column accessories (discs and caps), and one funnel (column reservoir extender)

Thermo Scientific™ Pierce Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as the percent of total purified protein mass.

The Glycoprotein Carbohydrate Estimation Kit includes six purified glycoprotein standards and the required assay reagents to effectively estimate the amount of oxidizable glycosylation (% carbohydrate by weight) of purified protein samples. Sugar groups in the glycoprotein sample are first oxidized with sodium meta-periodate to produce detectable aldehyde groups. Then the sample is reacted with the colorimetric Glycoprotein Detection Reagent. Absorbance at 550nm of the resulting purple reaction product is measured with a spectrophotometer or plate reader. Finally, the carbohydrate content in the glycoprotein is calculated by comparison to results from the five glycoprotein standards that are included in the kit.

Highlights:

• Qualitative – easily identifies purified proteins as glycoproteins or samples as contaminated with sugars
• Semi-quantitative – estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards
• Simple procedure – completed in less than 75 minutes; instructions include microplate and test tube protocols
• Adaptable – standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components

Includes:

Sodium periodate, assay buffer, detection reagent and a 5-vial set of glycoprotein standards

Recommended for:

Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents; Characterize and classify purified protein fractions from an affinity procedure; Assess sugar and carbohydrate contamination in non-glycosylated protein samples

Thermo Scientific™ Pierce Heptafluorobutyric Acid (HFBA) is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

Highlights:

• Typical purity is 99.7% by GC; <0.1% water
• Sequencing reagent for classical and automated Edman degradation of peptides and proteins
• Density: 1.645
• B.P. 120°C
• Packaged under nitrogen in amber glass ampules or bottles
• Clear, colorless liquid

Ordering Information:

X in the ordering table indicates that hazardous shipping charges apply.

Thermo Scientific™ Pierce Heptafluorobutyric Acid (HFBA) is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

Highlights:

• Typical purity is 99.7% by GC; <0.1% water
• Sequencing reagent for classical and automated Edman degradation of peptides and proteins
• Density: 1.645
• B.P. 120°C
• Packaged under nitrogen in amber glass ampules or bottles
• Clear, colorless liquid

Ordering Information:

X in the ordering table indicates that hazardous shipping charges apply

Thermo Scientific™ Pierce PolyHis Protein Interaction Pull-Down Kit purifies protein interaction complexes involving practically any user-supplied His-tagged fusion protein.

The PolyHis Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with recombinant proteins containing a histidine (6xHis) fusion tag. You provide the tagged fusion protein (bait) and cells expressing the putative protein interaction target (prey), and the pull-down kit supplies everything else: cell lysis buffer, microcentrifuge spin columns, cobalt-IMAC affinity resin (agarose beads), optimized binding and elution buffers, and a detailed protocol. This kit is designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.

Highlights:

• 6xHis pull-down – purifies protein interactors of any His-tagged fusion protein
• Complete kit – provides all components and detailed protocol for purifying protein:protein interactions
• No special equipment needed – uses common laboratory equipment and reagents (e.g., microcentrifuge)
• Convenient – microcentrifuge spin columns facilitate simple and efficient manipulation of cobalt IMAC agarose beads, including simple processing of multiple samples
• Flexible – instructions include protocols for bait and prey proteins from different sources

Includes:

Cobalt chelate agarose resin, lysis buffer, TBS pack, imidazole solution for elution, microcentrifuge spin columns and collection tubes

Recommended for:

Discover a new protein:protein interaction from a cell lysate; Confirm a putative interaction from a cell lysate or with a previously purified protein; Extract protein:protein interaction information from in vitro transcription/translation lysates