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Tetramethylammonium Hydroxide Pentahydrate

Tetramethylammonium Hydroxide Pentahydrate

Tetramethylammonium Hydroxide Pentahydrate

Tetramisole hydrochloride

Tetrandrine, NeuroPure

Tetrandrine, NeuroPure

Thalidomide

Thalidomide

Thalidomide

THAPSIGARGICIN

THAPSIGARGICIN

Thapsigargin

Thapsigargin

Thapsigargin (high purity)

THENOYLTRIFLUOROACETONE

THENOYLTRIFLUOROACETONE

THENOYLTRIFLUOROACETONE

Theophylline, anhydrous

Theophylline, anhydrous

Theophylline, anhydrous

Theophylline, anhydrous

Chloramine-T trihydrate, >-97%, C7H14ClNNaO5S, CAS Number-7080-50-4, 5kg, 97+%, White, 192 deg.C, 281.69, GHS Signal Word: Danger.

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Increase your lab’s efficiency with the variable speed Thermo Scientific™ Tube Revolver / Rotator. A small footprint, flexible design and simple display help increase flexiblity and effectiveness.

• • Interchangeable rotisseries accommodate different tube sizes for maximum flexiblity. Each paddle can be adjusted from 0-90 degrees.

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• • Achieve more effective mixing with oscillating mode. Oscillation will occur at every 40 degrees in the rotation cycle.

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• • Small footprint

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• • Plug-and-play simplicity

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• • Maintenance-free motor, quiet operation

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Warranty and Services

Two-year warranty.

Thermo Scientific™ Easy-Titer Antibody Assay Kits enable accurate determination of antibody concentration in serum and culture supernatant, and include kits specific for mouse IgG, rabbit IgG, human IgG (H+L), human IgG (Fc), or human IgM.

nThese kits include antibody-sensitized microspheres to measure the specific concentration of mouse, rabbit and human antibodies by an easy and rapid micro-agglutination technique using standard microplates and UV-Vis plate reader (spectrophotometer). Each kit is specific for a particular species and class of immunoglobulin and, unlike total protein assays, can specifically measure the concentration of target antibody in samples (e.g., serum, plasma, culture supernatant) that contain other proteins. The kits are extremely sensitive, requiring very small sample volumes. Antibody concentration is determined from the assay response (absorbance) by comparison to a standard curve prepared using dilutions of a known antibody sample (sold separately).

Thermo Scientific™ Pierce BCA Protein Assay Kit remains one of the most popular protein quantitation methods worldwide.

Thermo Scientific™ Pierce Dimethylsulfoxide (DMSO) is a sequencing-grade preparation with high purity and consistent absorptivity properties for use in HPLC and mass spectrometry methods.nn nnA sequencing-grade preparation with high purity for use in traditional amino acid analyzers and other high-volume applications.nnThis sequencing-grade dimethylsulfoxide is >99.5% pure DMSO. The economically priced 950mL bottles are an excellent choice for protein and peptide applications that requiring large volumes of the water-miscible solvent. The 50mL bottles are provide convenience and minimize waste for small dissolution and reaction volumes. The purity test ensures that preparation is essentially devoid of amines. The formulation was originally developed for peptide sequencing applications with amino acid analyzers, which required high purity solvent and an affordable price. This DMSO is packaged in solvent-rinsed amber glass bottles and sealed with PTFE-lined fluorocarbon caps for ultimate protection.

Perform a variety of antibody affinity purification methods with Thermo Scientific™ Pierce Protein A Agarose, a standard-capacity Protein A beaded agarose resin.nn nnPierce Protein A Agarose consists of purified native Protein A that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose. The agarose beads have physical and chemical properties that enable them to be used in a variety of batch- or column-type affinity purification procedure. Several package formats are offered: 5mL and 25mL bottles of resin slurry, 1mL pre-packed columns and a complete IgG purification kit.nnProtein A is a bacteria-derived protein that binds with high affinity and specificity to the Fc portion of antibodies, especially those of the IgG class. Compared to its alternative (Protein G), Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs and cats. Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly. Protein A also binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype.

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.

nTMB (3,3′,5,5′ tetramethylbenzidine) is a chromagen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) with major absorbances at 370 and 652nm. The color then changes to yellow (measured at 450nm) upon addition of sulfuric or phosphoric acid to stop the reaction. TMB is very sensitive and more quickly oxidized than other HRP substrates, resulting in faster color development. Unlike other commercially available substrates, these products contain no DMF or DMSO. There are three formulations that differ primarily in their sensitivities. Slow TMB is intermediate in sensitivity, ideal for kinetic readings. The sensitivity of the Turbo TMB compares to that of OPD used at approximately 1mg/mL. Ultra TMB-ELISA produces the highest signal-to-noise ratio and sensitivity in the picogram range. Highlights:

• • Ready-to-use solutions

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• • No hydrogen peroxide required

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• • No filtering required

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• • Noncarcinogenic

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• • Various sensitivities to suit any assay

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n1-Step TMB Substratesn

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  • Ready-to-use, one-component substrates

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• • Do not require hydrogen peroxide

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• • Noncarcinogenic

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• • Do not contain DMF or DMSO

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• • Available in three formulations that vary by sensitivity

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nTMB Substrate Kitn

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  • More sensitive than OPD in endpoint ELISAs

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• • Easy to use with results in seconds

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• • Ipon receipt store at 4°C, protected from moisture

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nIncludes:nnTMB Substrate Kit (PI-34021): Peroxidase substrate, 200 mL; Peroxide solution, 200 mL

Perform DNA and protein quantitation in 260nm and 280nm range with Thermo Scientific™ Nunc™ 96-Well UV Microplates. The 96-Well format allows for a higher throughput of sample screening compared to cuvettes.

Thermo Scientific™ 96-Well Microtiter Microplates are ideal for micro-volume applications such as storage, sample or reagent transfer, serial dilution, hemagglutination, blood typing and compliment fixation.

Features:

• Use for high-throughput screening and research
• Clear plates for colorimetric applications
• Standard footprint, 128 × 86mm
• Available as both polystyrene square wells and vinyl square wells
• Vinyl Microtiter microplates are used for complement fixation, agglutination and blood typing
• Styrene Microtiter microplates are used for complement fixation, agglutination, microbiology testing and blood typing

Ordering Information:

Polystyrene microplates are available as 50 units/package. Vinyl microplates are available as 25 or 100 units/package, depending on product.

Store samples and perform agglutination assays with Thermo Scientific™ Nunc™ 96 DeepWell™ Polystyrene Plates, featuring transparent round bottom deep wells that hold up to 900µL per well.

• • Alphanumeric grid provides quick and easy sample identification.

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• • Optimized for robotics and liquid handling systems

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• • Round-bottom wells for optimal sample recovery

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• • Untreated surface

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• • Sealable with well caps and sealing tape

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• • Customized bar coding is available

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This reducing agent-compatible (RAC) version of the Thermo Scientific™ Pierce BCA Protein Assay provides accurate determination of protein concentration in samples that contain dithiothreitol (DTT), 2-mercaptoethanol (BME), TCEP and other disulfide reducing agents.

The BCA Protein Assay Kit – Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA Assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, 2-ME or TCEP. Available in tube and microplate formats, these reducing agent compatible (RAC) BCA Kits extend the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.

Highlights:

• Compatible – assay samples that contain up to 5mM DTT, 35mM BME, or 10mM TCEP
• BCA technology – only a slight modification of the standard BCA Protein Assay protocol (15-minute incubation with Compatibility Reagent); no precipitation steps required
• Small samples – requires only 25μL (standard kit) or less than 10μL (microplate kit) of sample
• Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)
• Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods
• High linearity – linear working range for BSA equals 125 to 2000μg/mL

Includes:

BCA Reagents A and B, Compatibility Reagent, Reconstitution Buffer, and Albumin Standard (2mg/mL); Microplate kit also includes assay-compatible 96-well microplates

ActivX™ Desthiobiotin-GTP Probes contain a modified biotin attached to the nucleotide by a labile acyl-phosphate bond. ActivX active-site enrichment probes are advantageous for determining active enzyme levels through the use of analog-tagged probes to allow profiling of both inactive and active enzymes. The ActivX probes selectively enrich only those enzymes that are functionally active and biologically relevant at the time of labeling. With selective enrichment, one can identify and profile target enzyme classes across samples or can assess the specificity and affinity of enzyme inhibitors.

After removal of GTP or GDP nucleotides from enzymes, the desthiobiotin-GTP probe can be used to covalently modify conserved lysine residues in the GTPase nucleotide-binding site. Desthiobiotin-GTP can selectively enrich, identify and profile target enzyme classes in samples. Pre-incubation of samples with small-molecule inhibitors that compete with active-site probes can be used to determine inhibitor binding affinity and target specificity. The ActivX Desthiobiotin-GTP Probe covalently labels the active site of GTPases and GTPase subunits of G-protein coupled receptors.

Applications of ActivX Desthiobiotin-GTP Probe:
• Broad enrichment of GTP-binding proteins from tissues, cells and sub-cellular proteomes
• Enrichment of enzymes based on function
• Profiling of dozens to hundreds of inhibitor targets

Assessment of active-site labeling with desthiobioin probes can be accomplished by either Western blot or mass spectrometry (MS). For the Western blot workflow, desthiobiotin-labeled proteins are enriched for SDS-PAGE analysis and subsequent detection with specific antibodies. For the MS workflow, desthiobiotin-labeled proteins are reduced, alkylated and enzymatically digested to peptides. Only the desthiobiotin-labeled, active-site peptides are enriched for analysis by LC-MS/MS. Both workflows can be used for determining inhibitor target binding, but only the MS workflow can identify global inhibitor targets and off-targets.

These products are subject to a limited use label license.

Thermo Scientific™ Pierce Agaroses are highly purified and carefully blended formulations of regular- or low-melting agarose that provide uniform lot-to-lot consistency for preparation of electrophoresis gels.

Highlights:

• Agarose I (Molecular Biology Grade)
• A regular melting-temperature agarose for electrophoresis requiring an exceptional level of purity with unequaled performance
• Agarose II (Low Melt)
• A low temperature agarose for special applications requiring a lower melting temperature
• Optimal for “in-gel” restriction digestion/ligation and DNA recovery
• At 1%, forms a very manageable clear gel that provides excellent resolution and melting characteristics
• Melts at approximately 65°C and remains fluid at 37°C for at least 24 hours, providing an excellent matrix for enzymatic manipulation of samples

Thermo Scientific™ Pierce Agaroses are highly purified and carefully blended formulations of regular- or low-melting agarose that provide uniform lot-to-lot consistency for preparation of electrophoresis gels.

Highlights:

• Agarose I (Molecular Biology Grade)
• A regular melting-temperature agarose for electrophoresis requiring an exceptional level of purity with unequaled performance
• Agarose II (Low Melt)
• A low temperature agarose for special applications requiring a lower melting temperature
• Optimal for “in-gel” restriction digestion/ligation and DNA recovery
• At 1%, forms a very manageable clear gel that provides excellent resolution and melting characteristics
• Melts at approximately 65°C and remains fluid at 37°C for at least 24 hours, providing an excellent matrix for enzymatic manipulation of samples

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Thermo Scientific™ Protease Inhibitors are small packages of individual protease inhibitor peptides and compounds for customized formulation or modification of protease inhibitor cocktails.

The individual reagents include AEBSF, aprotinin, bestatin, E64, leupeptin, pepstatin A, and PMSF. Although Thermo Scientific Halt™ Protease Inhibitor Cocktails provide convenient and optimized broad-spectrum protease inhibition for routine cell lysis and assay needs, certain applications require a more customized approach. By offering the individual high-quality reagents used to make our cocktail products, we provide you with the tools needed to apply a protease inhibitor individually, supplement a cocktail, or combine several components to make customized protease inhibitor cocktails for specialized applications.

Highlights:

• High-quality reagents in convenient and affordable package sizes
• Purchase individually to prepare customized protease inhibitor cocktails
• Supplement Halt Protease Inhibitor Single-Use Cocktails, whose formulations are fully disclosed, to achieve desired concentrations of specific component reagents

Perform quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ Black 96-Well Immuno Plates. Featuring a choice of three surface types and optimal binding, these plates minimize background in fluorescence reading.   Features:

• • Plate has SBS footprint — fits standard equipment

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• • Available with flat- or round-bottom wells

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• • Certified binding homogeneity/reproducibility

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nChoice of surface:n

• • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) are for absorption of hydrophobic molecules.

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• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays.

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• • Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules.

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nCompatible with:nnCompatible with lids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane