All Products shortcode with no Uncategorized products. – (SHOP test speed)

Thermo Scientific™ Pierce Sulfo-EMCS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 Ås).

Highlights:

• Water-soluble
• Cleavable at pH 8.5 using hydroxylamine and heat
• Lactose dehydrogenase retained 60% of its activity after reversible crosslinking
• Reactive groups: sulfo-NHS esters (homobifunctional)
• Reactive toward: amino groups

Thermo Scientific™ Pierce Sulfo-SMPB is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 Ås).

Highlights:

• Extended chain length limits steric hindrance
• Water-soluble; membrane-impermeable
• Reactive groups: sulfo-NHS ester and maleimide
• Reactive toward: amino and sulfhydryl groups

Thermo Scientific™ SuperBlock Blocking Buffers are optimized PBS and TBS solutions containing a protein that provides incredibly fast and effective blocking for ELISA, immunohistochemistry and Western blotting applications.

nBlocking microplates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.nnHighlights:n

• • Fast – block membranes in 5 to 10 minutes and ELISA plates in 2 minutes

• • Flexible – guaranteed to be biotin-free for use with streptavidin system

• • Convenience – available in multiple buffer formats (in PBS or TBS with or without Tween™-20 detergent) and package sizes (1L, 5L and dry-blend pouches)

• • Low background – the non-serum protein solution yields a high signal-to-noise ratio

• • Stable – store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Description

Thermo Scientific™ SuperSignal West Femto Substrate is an ultra-sensitive enhanced chemiluminescent substrate (ECL substrate) for detection of peroxidase activity from HRP-conjugated antibodies and probes in Western blotting.

nSuperSignal West Femto Substrate Maximum Sensitivity Substrate is an ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme. The substrate kit enables detection of low femtogram (that’s mid-zeptomole!) amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are small to be seen with typical ECL substrates.nnHighlights:n

• n
  • ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

n

• • Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes

n

• • Quantitative – produces quantitative signal that is measurable over two orders of magnitude

n

• • Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks)

n

• • Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system

n

• • Excellent signal duration – 8 hours of usable light-output when conditions are optimized

n

• • Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature

n

Thermo Scientific™ SuperSignal West Femto Substrate is an ultra-sensitive enhanced chemiluminescent substrate (ECL substrate) for detection of peroxidase activity from HRP-conjugated antibodies and probes in Western blotting.

nSuperSignal West Femto Substrate Maximum Sensitivity Substrate is an ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme. The substrate kit enables detection of low femtogram (that’s mid-zeptomole!) amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are small to be seen with typical ECL substrates.nnHighlights:n

• n
  • ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

n

• • Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes

n

• • Quantitative – produces quantitative signal that is measurable over two orders of magnitude

n

• • Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks)

n

• • Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system

n

• • Excellent signal duration – 8 hours of usable light-output when conditions are optimized

n

• • Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature

n

• • Ready to use, no preparation required

n

• • Validated for use with Thermo Scientific Pierce Matched Antibody Pairs and MiniKits

n

• • Size: 100 mL (sufficient for about ten 96-well microplates)

n

6X TriTrack DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains three different dyes (bromophenol blue, xylene cyanol FF or orange G) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solutions binds divalent metal ions and inhibits metal-dependent nucleases.nn6X TriTrack DNA Loading Dye is used for conventional DNA electrophoresis.

Highlights.

• • Three-color tracking of DNA migration during DNA electrophoresis

n

• • No DNA masking during gel exposure to UV light

n

• • EDTA binds divalent metal ions and inhibits metal dependent nucleases

n

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility. Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• n
  • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

nCompatible with:nnLids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility.Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• n
  • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

nCompatible with:nnLids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility. Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• n
  • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

nCompatible with:nnLids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility. Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• n
  • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

nCompatible with:nnLids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility.

Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• n
  • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

nCompatible with:nnLids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility.

Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

Compatible with: Lids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Thermo Scientific™ Zeba Spin Desalting Columns (7K MWCO) contain a size-exclusion chromatography resin that provides excellent protein desalting performance in a centrifuge format for 2µL to 4mL samples.

nThese specially designed polypropylene centrifuge columns are pre-packed with high-performance Zeba Desalting Resin to facilitate rapid protein desalting with high recovery. The resin effectively traps salts and other small sample components but does not impede proteins and other macromolecules, which pass directly into the centrifuge collection tube. These particular columns use Zeba Resin having a molecular-weight cutoff (MWCO) rating equal to 7K; this ensures that proteins larger than 7000 MW will be recovered with high efficiency.nnThe easy-to-use spin column format dramatically improves results over standard drip-column methodologies. This system eliminates the need to wait for samples to emerge by gravity flow and to monitor fractions for protein recovery. Samples are processed in these desalting columns using a standard centrifuge or microcentrifuge. No chromatography hardware or column preparation steps are required. Multiple samples can be processed in less than 10 minutes. Zeba Spin Desalting Columns are available in five different column sizes, and these provide trouble-free desalting and buffer exchange for sample volumes ranging from 2µL to 4mL

Highlights:

• • Fast centrifuge format – resin and columns are optimized for rapid processing by centrifugation with standard collection tubes and rotors

• • Replaces drip-column methods – no need to collect and screen slowly emerging protein fractions

• • Exceptional protein yields – recover greater than 95% in typical applications with minimal dilution

• • Five convenient sizes – columns with 75µL to 10mL of Zeba Resin provide desalting matched for sample volumes between 2µL and 4mL

• • 7K MWCO – resin is rated to recover proteins larger than 7000 daltons and retain (remove) solutes smaller than 1000 MW

Requires:nnMicrocentrifuge and microcentrifuge collection tubes (for 75µL and 0.5mL columns) or centrifuge and collection tubes (15mL tubes for 2 and 5mL columns; 50mL tubes for 10mL columns)

This lyophilized enzyme is from Bacillus thermoproteolyticus. This enzyme is characterized by its excellent heat stability and substrate specificity towards isoleucine, methionine and valine. Activity: 7000 PU/mg of protein.

THIAMINE CONTROL DIET, Modified

THIAMINE CONTROL DIET, Modified

THIAMINE CONTROL DIET, Modified

Thiamine Control Diet, Modified

Thiamine Control Diet, Modified

Thiamine Control Diet, Modified

THIAMINE DEFICIENT DIET, Modified

THIAMINE DEFICIENT DIET, Modified

THIAMINE DEFICIENT DIET, Modified

Thiamine Deficient Diet, Modified

Thiamine Deficient Diet, Modified

Thiamine Deficient Diet, Modified

Thiamine Hydrochloride

Thiamine Hydrochloride

Thiamine Hydrochloride

Thiamine, Hydrochloride

Thiamine, Hydrochloride

Thiamine, Hydrochloride

Thiamine, Hydrochloride

Thiamphenicol

Thiamphenicol

Thiazovivin, NeuroPure

Thiazovivin, NeuroPure

Thidiazuron, PhytoPure

Thidiazuron, PhytoPure