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TaqMan™ Fast Advanced Master Mix, no UNG SKU: A44360 X

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in a short time. It provides best-in-class performance, including the widest quantifiable range, in both single and duplex reactions, even with challenging targets. TaqMan Fast Advanced Master Mix contains AmpliTaq Fast DNA Polymerase, dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components. It is supplied at a 2X concentration. Note: This TaqMan Fast Advanced Master Mix does not contain uracil-N-glycoslyase (UNG). See a complete listing of all TaqMan Fast Advanced Master Mix products, with and without UNG.

TaqMan Fast Advanced Master Mix features include:
• Best-in-class performance—superior sensitivity, accuracy, dynamic range, and specificity compared to standard mixes in standard mode
• Engineered for enhanced benchtop stability—stable at room temperature for up to 72 hours in preassembled reactions
• Optimized for multiplexing—validated for duplexing with exogenous and endogenous internal positive control assays
• Reduced run times (<40 minutes) on fast and standard instrumentation—optimized for fast mode on fast instruments and fast cycling conditions on standard instruments
• Seamlessly transitions into your experiments—validated with TaqMan Assays for gene expression and microRNAs, and TaqMan array micro-fluidic cards

Best-in-class performance
TaqMan Fast Advanced Master Mix has been designed to provide performance equal to or better than standard master mixes. It has been benchmarked against the leading suppliers’ standard and fast master mixes to help ensure that it succeeds in providing best-in-class sensitivity, accuracy, dynamic range, and specificity. TaqMan Fast Advanced Master Mix’s dynamic range (up to 7 logs) is the widest in the industry. The impressive sensitivity of the mix is showcased in the figure below which compares the Ct values of TaqMan Fast Advanced Master Mix and TaqMan Universal PCR Master Mix across a panel of gene expression assays.

Benchtop stability for high-throughput handling and convenience
TaqMan Fast Advanced Master Mix has been engineered to retain a high level of performance in preassembled reactions for up to 72 hours. The stability of this mix provides users of high-throughput liquid handling systems the assurance that the results on the first plate will mimic those of the last plate. The figure below shows an assay that was run upon assembly (time 0) and after 72 hours of incubation at 30°C, simulating the most extreme room temperature scenario.

For those with less extreme throughput needs, the enhanced stability of this master mix provides an overall added convenience to your workflow, as you are no longer constrained to immediately running plates upon assembly.

Optimized for multiplexing
We realize that confidence is paramount when it comes to your results. TaqMan Fast Advanced Master Mix has been designed to help deliver accurate results for duplex reactions using an internal positive control (IPC). The figure below shows results for the experimental target ß-actin (gene name: ACTB), which was serially diluted and amplified in single-target reactions and duplex reactions.

Validated for microRNA assays
TaqMan Fast Advanced Master Mix has been validated for use with multiple real-time PCR applications, including microRNA assays. The formulation provides high specificity and dynamic range, the two most critical performance attributes that define successful results when working with microRNAs. The data in the figure below demonstrate excellent PCR linearity over a 6-log range of input template.

Reduced run times on standard instrumentation
TaqMan Fast Advanced Master Mix has been optimized for use with both fast and standard instrumentation, enabling researchers who currently own standard instruments to also reap the performance benefits and time savings this mix provides. The figure below showcases the impressive results achieved when using TaqMan Fast Advanced Master Mix under fast thermal cycling conditions on the Applied Biosystems 7300 Real-Time PCR System. The mix has been tested with all Applied Biosystems standard real-time PCR instrumentation to enable success independent of whether or not you own a fast-enabled instrument.

$1,061.54 Add to cart View Product Details

TaqMan™ MicroRNA Assay

Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with an miRNA-specific primer, followed by real-time PCR with TaqMan probes. TaqMan MicroRNA Assays are:

• Highly specific—quantitate only mature miRNAs, not precursors
• Sensitive—conserve limited samples, requires only 1–10 nanograms of total RNA or equivalent
• Fast, simple and scalable—two-step quantitative RT-PCR assay provides high-quality results in less than three hours

Our TaqMan MicroRNA Assay catalogue is kept up to date with the Sanger database to provide comprehensive coverage with the most current annotation.

Availability and sizes
Made-to-Order assays are available in four different sizes and usually ship within two weeks. Inventoried assays are prepackaged in Small size and are ready to ship. Inventoried assays may be purchased in larger sizes by ordering them as Made to Order.

Species
TaqMan MicroRNA Assays are available for a range of species including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis. We will continue to increase the number of miRNA assays for these species in alignment with the Sanger miRBase Registry.

Assay Components
TaqMan MicroRNA Assays are delivered in two tubes: one containing the RT primer, the other containing the specific pre-formulated TaqMan Assay (TaqMan probe and PCR primer set).

Pre-formulated assay
• Forward primer: 1500 nM
• Reverse primer: 700 nM
• FAM-MGB probe: 200 nM
• RT sequences: 50 nM

TaqMan miRNA Assay selection guide

TaqMan MicroRNA Assays
Description: TaqMan MicroRNA Assays employ a novel target-specific stem–loop primer during cDNA synthesis to produce a template for real-time PCR
RT chemistry: miRNA-specific RT
Throughput: Best for 1–10 targets
Coverage: 205 species available, coverage for miRBase v.21

TaqMan Advanced miRNA Assays
Description: TaqMan Advanced miRNA Assays employ a universal RT step for a streamlined workflow, and a universal miR-Amp step to enable highly sensitive detection by real-time PCR
RT chemistry: Universal RT
Throughput: Best for >10 targets
Coverage: All human, mouse, and rat miRNAs; coverage for miRBase v.24

$306.65 Add to cart View Product Details

TARTARIC ACID A.R. GRANU 2.5K – MACR2312-06, 2.5 kg

TARTARIC ACID A.R. GRANU 2.5K

$2,023.02 Add to cart View Product Details

TARTARIC ACID A.R. GRANU 2.5K – MACR2312-06, 2.5 kg

TARTARIC ACID A.R. GRANU 2.5K

$505.76 Add to cart View Product Details

TARTARIC ACID A.R. GRANU 500GM – MACR2312-04, 500 g

TARTARIC ACID A.R. GRANU 500GM

$1,897.94 Add to cart View Product Details

TARTARIC ACID A.R. GRANU 500GM – MACR2312-04, 500 g

TARTARIC ACID A.R. GRANU 500GM

$158.17 Add to cart View Product Details

TARTARIC ACID GRAN N.F.2500GM – BAKR4105-05, 2.5 kg

L -(+) TARTARIC ACID GRAN N.F. 2500GM

$950.98 Add to cart View Product Details

TARTARIC ACID GRAN NF 500G – BAKR4104-01, 500 g

TARTARIC ACID GRAN NF 500G

$280.44 Add to cart View Product Details

TARTARIC ACID N.F. 500GM – MACR2307-04, 500 g

TARTARIC ACID N.F. 500GM

$2,614.44 Add to cart View Product Details

TARTARIC ACID N.F. 500GM – MACR2307-04, 500 g

TARTARIC ACID N.F. 500GM

$217.87 Add to cart View Product Details

Taurine

$36.73 Add to cart View Product Details

Taurine

$66.71 Add to cart View Product Details

Taurine

$224.85 Add to cart View Product Details

Taurine

$389.19 Add to cart View Product Details

Taurocholic Acid Sodium Salt, Practical Grade

Taurocholic Acid Sodium Salt

$527.03 Add to cart View Product Details

Taurocholic Acid Sodium Salt, Practical Grade

Taurocholic Acid Sodium Salt

$1,594.15 Add to cart View Product Details

1,386.22

Taurocholic Acid Sodium Salt, Practical Grade

Taurocholic Acid Sodium Salt

$6,516.02 Add to cart View Product Details

5,666.10

TBE (5X), TRIS + Borate + EDTA

$49.50 Add to cart View Product Details

TBE (5X), TRIS + Borate + EDTA

$94.79 Add to cart View Product Details

TBE buffer

Tris-Borate-EDTA electrophoresis buffer, ready-to-use

$73.47 Add to cart View Product Details

TBE buffer

Tris-Borate-EDTA electrophoresis buffer, ready-to-use

$168.14 Add to cart View Product Details

TBE Buffer, 10X

Buffer for nucleic acid electrophoresis.

$68.43 Add to cart View Product Details

TBE Buffer, 10X

Buffer for nucleic acid electrophoresis.

$354.74 Add to cart View Product Details

TBE Modifier mixture

Proprietary mixture of concentrated salts for removing DNA from TBE-buffered gels. Used with NaI.

$47.18 Add to cart View Product Details

TBE Running Buffer (10X)

$68.15 Add to cart View Product Details

TBE Sample Buffer (6X)

$86.04 Add to cart View Product Details

TBE Sample Buffer (6X)

$145.60 Add to cart View Product Details

TBE Urea Sample Buffer (2X)

$86.04 Add to cart View Product Details

TBE Urea Sample Buffer (2X)

$158.84 Add to cart View Product Details

TCH® defined serum replacement, 50X concentrate

TCH® is a fortified, multipurpose serum replacement for long-term culturing of primarily human cells and the production of cell secreted proteins. TCH is packaged as a 50X concentrate.

$211.74 Add to cart View Product Details

TCLP 110MM 50/PK

$191.41 Add to cart View Product Details

TCLP 125MM 50/PK

$284.03 Add to cart View Product Details

TCLP 14.2CM 100/PK

$344.01 Add to cart View Product Details

TCLP 142MM 50/PK

$345.77 Add to cart View Product Details

TCLP 150MM 50/PK

$384.58 Add to cart View Product Details

TCLP 47MM 100/PK

$164.57 Add to cart View Product Details

TCLP 90MM 50/PK

$131.10 Add to cart View Product Details

TCLP GLASS 0.7um 293MM 25/PK

$422.23 Add to cart View Product Details

TCM® Defined Serum Replacement

TCM™ is a fortified, multipurpose serum replacements for the long-term culturing of anchorage dependent and suspension cells from a variety of species. TCM™ is packaged as a 50X concentrate.

$205.49 Add to cart View Product Details