All Products shortcode with no Uncategorized products. – (SHOP test speed)

application (WB, ELISA) & cross reactivity (Mouse)

application (WB, ELISA) & cross reactivity (Mouse)

application (WB, IF-P, IHC-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF-P, IHC-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, IF-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, IF-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, ELISA) & cross reactivity (Mouse)

application (WB, IF/ICC, ELISA) & cross reactivity (Mouse)

application (WB, IHC-P, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IHC-P, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, ELISA) & cross reactivity (Human)

application (WB, ELISA) & cross reactivity (Human)

application (WB, ELISA) & cross reactivity (Human, Mouse)

application (WB, ELISA) & cross reactivity (Human, Mouse)

application (WB, IHC-P, FC (intra), ELISA) & cross reactivity (Human)

application (WB, IHC-P, FC (intra), ELISA) & cross reactivity (Human)

application (WB, IHC-P, IP, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IHC-P, IP, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It can catalyze the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. T3 DNA Ligase will join blunt end and cohesive end termini. It and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids and close the gaps in these DNA substrates. In addition, T3 DNA Ligase is more tolerant to NaCl than T4 DNA Ligase (2-fold).
As with T4 DNA Ligase , addition of PEG 6000 to the T3 DNA Ligase reaction system can improve the ligation efficiency of the blunt end. 1X T4 DNA Ligase Reaction Buffer can be used in some experiments where PEG 6000 is not available, but the activity of T3 DNA Ligase is reduced to 1/10. In applications where high concentrations of NaCl need to be maintained, we recommend the use of reaction buffers without PEG 6000.

T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It can catalyze the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. T3 DNA Ligase will join blunt end and cohesive end termini. It and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids and close the gaps in these DNA substrates. In addition, T3 DNA Ligase is more tolerant to NaCl than T4 DNA Ligase (2-fold).
As with T4 DNA Ligase , addition of PEG 6000 to the T3 DNA Ligase reaction system can improve the ligation efficiency of the blunt end. 1X T4 DNA Ligase Reaction Buffer can be used in some experiments where PEG 6000 is not available, but the activity of T3 DNA Ligase is reduced to 1/10. In applications where high concentrations of NaCl need to be maintained, we recommend the use of reaction buffers without PEG 6000.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5’→3′ direction and requires the presence of template and primer. This enzyme has a 3’→5′ exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5’→3′ exonuclease function.
It is applicable to 3′ overhang removal to form blunt ends, 5′ overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis.

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5’→3′ direction and requires the presence of template and primer. This enzyme has a 3’→5′ exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5’→3′ exonuclease function.
It is applicable to 3′ overhang removal to form blunt ends, 5′ overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis.

T4 Gene 32 Protein (10 mg/mL), also known as T4 gp32 protein, is a single stranded specific DNA binding protein. The native Gene 32 Protein from bacteriophage T4 (T4gp32) is a single-stranded DNA binding protein that is required for T4 DNA replication, recombination and repair. T4 Gene 32 protein can increase the yield of long PCR products when the reaction contains proteins ranging from 0.5 to 1.0 nmol. The T4 Gene 32 protein can increase the production of PCR products，reducing the inhibitor influence of humic acid. Meanwhile, the protein can promote the digestion reaction of restriction endonucleases, reverse transcription efficiency in RT-PCR, and enhance the activity of T4 DNA polymerase.

T4 Gene 32 Protein (10 mg/mL), also known as T4 gp32 protein, is a single stranded specific DNA binding protein. The native Gene 32 Protein from bacteriophage T4 (T4gp32) is a single-stranded DNA binding protein that is required for T4 DNA replication, recombination and repair. T4 Gene 32 protein can increase the yield of long PCR products when the reaction contains proteins ranging from 0.5 to 1.0 nmol. The T4 Gene 32 protein can increase the production of PCR products，reducing the inhibitor influence of humic acid. Meanwhile, the protein can promote the digestion reaction of restriction endonucleases, reverse transcription efficiency in RT-PCR, and enhance the activity of T4 DNA polymerase.

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates. T4 Polynucleotide Kinase also catalyzes the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates.
T4 PNK is applicable to end-labeling DNA or RNA for probes and DNA sequencing, addition of 5´-phosphates to oligonucleotides to allow subsequent ligation and removal of 3´-phosphoryl groups.

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates. T4 Polynucleotide Kinase also catalyzes the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates.
T4 PNK is applicable to end-labeling DNA or RNA for probes and DNA sequencing, addition of 5´-phosphates to oligonucleotides to allow subsequent ligation and removal of 3´-phosphoryl groups.

T4 RNA ligase 1 catalyzes the formation of a 3´ → 5´ phosphodiester bond by joining the 5´ phosphate end of a nucleotide to the 3´ hydroxyl end, accompanied by the hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA, DNA, and dinucleoside pyrophosphates.

T4 RNA ligase 1 catalyzes the formation of a 3´ → 5´ phosphodiester bond by joining the 5´ phosphate end of a nucleotide to the 3´ hydroxyl end, accompanied by the hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA, DNA, and dinucleoside pyrophosphates.

T4 RNA Ligase 2, is an ATP-dependent double-stranded RNA ligase (dsRNA ligase), also known as T4 Rnl2 (gp24.1), which can be used for intramolecular circularization and intermolecular linear ligation of double-stranded RNA. Unlike T4 RNA Ligase 1, T4 RNA Ligase 2 exhibits significantly higher activity in connecting gaps (nicks) in double-stranded RNA compared to connecting ends of single-stranded RNA. T4 RNA Ligase 2 is also applicable for intra- or inter-molecular ligation of the 3′ hydroxyl of RNA chains and the 5′ phosphate of DNA chains within double-stranded nucleic acids (such as RNA double strands, RNA/DNA hybrids, and/or DNA double strands). The enzyme requires an adjacent 5´ phosphate and 3´ OH for ligation. The enzyme can also ligate the 3´ OH of RNA to the 5´ phosphate of DNA in a double stranded structure.

T4 RNA Ligase 2, is an ATP-dependent double-stranded RNA ligase (dsRNA ligase), also known as T4 Rnl2 (gp24.1), which can be used for intramolecular circularization and intermolecular linear ligation of double-stranded RNA. Unlike T4 RNA Ligase 1, T4 RNA Ligase 2 exhibits significantly higher activity in connecting gaps (nicks) in double-stranded RNA compared to connecting ends of single-stranded RNA. T4 RNA Ligase 2 is also applicable for intra- or inter-molecular ligation of the 3′ hydroxyl of RNA chains and the 5′ phosphate of DNA chains within double-stranded nucleic acids (such as RNA double strands, RNA/DNA hybrids, and/or DNA double strands). The enzyme requires an adjacent 5´ phosphate and 3´ OH for ligation. The enzyme can also ligate the 3´ OH of RNA to the 5´ phosphate of DNA in a double stranded structure.

T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA. The enzyme does not require ATP for ligation but does need the pre-adenylated substrate. T4 Rnl2 truncated is expressed from a plasmid in E. coli which encodes the first 249 amino acids of the full length T4 RNA Ligase 2. Unlike the full length ligase, T4 Rnl2 truncated is unable to adenylate the 5´ end of the substrate, and as a result it cannot ligate the phosphorylated 5´ end of RNA or DNA to the 3´ end of RNA. This enzyme, also known as Rnl2 (1-249) has been used for optimized linker ligation for the cloning of microRNAs. This enzyme reduces background ligation because it can only use adenylated primers.

T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA. The enzyme does not require ATP for ligation but does need the pre-adenylated substrate. T4 Rnl2 truncated is expressed from a plasmid in E. coli which encodes the first 249 amino acids of the full length T4 RNA Ligase 2. Unlike the full length ligase, T4 Rnl2 truncated is unable to adenylate the 5´ end of the substrate, and as a result it cannot ligate the phosphorylated 5´ end of RNA or DNA to the 3´ end of RNA. This enzyme, also known as Rnl2 (1-249) has been used for optimized linker ligation for the cloning of microRNAs. This enzyme reduces background ligation because it can only use adenylated primers.

T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q), specifically catalyzes the ligation of a 5´-adenylated DNA or RNA to the 3´ OH end of an RNA molecule. This enzyme-mediated ligation does not require ATP but necessitates a pre-adenylated adapter. T4 RNA Ligase 2, truncated KQ, a double-point mutant of T4 RNA Ligase 2 with R55K and K227Q substitutions, significantly reduces the non-specific ligation of RNA (such as concatenation or self-circularization) compared to T4 RNA Ligase 2, truncated, while maintaining enzyme activity similar to that of T4 RNA Ligase 2, truncated.