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application (WB, IF/ICC, IHC-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, IHC-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, ELISA) & cross reactivity (Mouse)

application (WB, ELISA) & cross reactivity (Mouse)

application (WB, IF-P, IHC-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF-P, IHC-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, IF-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, IF-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, ELISA) & cross reactivity (Mouse)

application (WB, IF/ICC, ELISA) & cross reactivity (Mouse)

application (WB, IHC-P, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IHC-P, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, ELISA) & cross reactivity (Human)

application (WB, ELISA) & cross reactivity (Human)

application (WB, ELISA) & cross reactivity (Human, Mouse)

application (WB, ELISA) & cross reactivity (Human, Mouse)

application (WB, IHC-P, FC (intra), ELISA) & cross reactivity (Human)

application (WB, IHC-P, FC (intra), ELISA) & cross reactivity (Human)

application (WB, IHC-P, IP, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IHC-P, IP, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It can catalyze the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. T3 DNA Ligase will join blunt end and cohesive end termini. It and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids and close the gaps in these DNA substrates. In addition, T3 DNA Ligase is more tolerant to NaCl than T4 DNA Ligase (2-fold).
As with T4 DNA Ligase , addition of PEG 6000 to the T3 DNA Ligase reaction system can improve the ligation efficiency of the blunt end. 1X T4 DNA Ligase Reaction Buffer can be used in some experiments where PEG 6000 is not available, but the activity of T3 DNA Ligase is reduced to 1/10. In applications where high concentrations of NaCl need to be maintained, we recommend the use of reaction buffers without PEG 6000.

T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It can catalyze the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. T3 DNA Ligase will join blunt end and cohesive end termini. It and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids and close the gaps in these DNA substrates. In addition, T3 DNA Ligase is more tolerant to NaCl than T4 DNA Ligase (2-fold).
As with T4 DNA Ligase , addition of PEG 6000 to the T3 DNA Ligase reaction system can improve the ligation efficiency of the blunt end. 1X T4 DNA Ligase Reaction Buffer can be used in some experiments where PEG 6000 is not available, but the activity of T3 DNA Ligase is reduced to 1/10. In applications where high concentrations of NaCl need to be maintained, we recommend the use of reaction buffers without PEG 6000.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5’→3′ direction and requires the presence of template and primer. This enzyme has a 3’→5′ exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5’→3′ exonuclease function.
It is applicable to 3′ overhang removal to form blunt ends, 5′ overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis.