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application (WB, IF/ICC, IHC-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, IHC-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, ELISA) & cross reactivity (Mouse)

application (WB, ELISA) & cross reactivity (Mouse)

application (WB, IF-P, IHC-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF-P, IHC-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, IF-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, IF-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, ELISA) & cross reactivity (Mouse)

application (WB, IF/ICC, ELISA) & cross reactivity (Mouse)

application (WB, IHC-P, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IHC-P, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, ELISA) & cross reactivity (Human)

application (WB, ELISA) & cross reactivity (Human)

application (WB, ELISA) & cross reactivity (Human, Mouse)

application (WB, ELISA) & cross reactivity (Human, Mouse)

application (WB, IHC-P, FC (intra), ELISA) & cross reactivity (Human)

application (WB, IHC-P, FC (intra), ELISA) & cross reactivity (Human)

application (WB, IHC-P, IP, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IHC-P, IP, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It can catalyze the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. T3 DNA Ligase will join blunt end and cohesive end termini. It and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids and close the gaps in these DNA substrates. In addition, T3 DNA Ligase is more tolerant to NaCl than T4 DNA Ligase (2-fold).
As with T4 DNA Ligase , addition of PEG 6000 to the T3 DNA Ligase reaction system can improve the ligation efficiency of the blunt end. 1X T4 DNA Ligase Reaction Buffer can be used in some experiments where PEG 6000 is not available, but the activity of T3 DNA Ligase is reduced to 1/10. In applications where high concentrations of NaCl need to be maintained, we recommend the use of reaction buffers without PEG 6000.

T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It can catalyze the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. T3 DNA Ligase will join blunt end and cohesive end termini. It and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids and close the gaps in these DNA substrates. In addition, T3 DNA Ligase is more tolerant to NaCl than T4 DNA Ligase (2-fold).
As with T4 DNA Ligase , addition of PEG 6000 to the T3 DNA Ligase reaction system can improve the ligation efficiency of the blunt end. 1X T4 DNA Ligase Reaction Buffer can be used in some experiments where PEG 6000 is not available, but the activity of T3 DNA Ligase is reduced to 1/10. In applications where high concentrations of NaCl need to be maintained, we recommend the use of reaction buffers without PEG 6000.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5’→3′ direction and requires the presence of template and primer. This enzyme has a 3’→5′ exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5’→3′ exonuclease function.
It is applicable to 3′ overhang removal to form blunt ends, 5′ overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis.

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5’→3′ direction and requires the presence of template and primer. This enzyme has a 3’→5′ exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5’→3′ exonuclease function.
It is applicable to 3′ overhang removal to form blunt ends, 5′ overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis.

T4 Gene 32 Protein (10 mg/mL), also known as T4 gp32 protein, is a single stranded specific DNA binding protein. The native Gene 32 Protein from bacteriophage T4 (T4gp32) is a single-stranded DNA binding protein that is required for T4 DNA replication, recombination and repair. T4 Gene 32 protein can increase the yield of long PCR products when the reaction contains proteins ranging from 0.5 to 1.0 nmol. The T4 Gene 32 protein can increase the production of PCR products，reducing the inhibitor influence of humic acid. Meanwhile, the protein can promote the digestion reaction of restriction endonucleases, reverse transcription efficiency in RT-PCR, and enhance the activity of T4 DNA polymerase.

T4 Gene 32 Protein (10 mg/mL), also known as T4 gp32 protein, is a single stranded specific DNA binding protein. The native Gene 32 Protein from bacteriophage T4 (T4gp32) is a single-stranded DNA binding protein that is required for T4 DNA replication, recombination and repair. T4 Gene 32 protein can increase the yield of long PCR products when the reaction contains proteins ranging from 0.5 to 1.0 nmol. The T4 Gene 32 protein can increase the production of PCR products，reducing the inhibitor influence of humic acid. Meanwhile, the protein can promote the digestion reaction of restriction endonucleases, reverse transcription efficiency in RT-PCR, and enhance the activity of T4 DNA polymerase.

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates. T4 Polynucleotide Kinase also catalyzes the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates.
T4 PNK is applicable to end-labeling DNA or RNA for probes and DNA sequencing, addition of 5´-phosphates to oligonucleotides to allow subsequent ligation and removal of 3´-phosphoryl groups.

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates. T4 Polynucleotide Kinase also catalyzes the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates.
T4 PNK is applicable to end-labeling DNA or RNA for probes and DNA sequencing, addition of 5´-phosphates to oligonucleotides to allow subsequent ligation and removal of 3´-phosphoryl groups.

T4 RNA ligase 1 catalyzes the formation of a 3´ → 5´ phosphodiester bond by joining the 5´ phosphate end of a nucleotide to the 3´ hydroxyl end, accompanied by the hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA, DNA, and dinucleoside pyrophosphates.

T4 RNA ligase 1 catalyzes the formation of a 3´ → 5´ phosphodiester bond by joining the 5´ phosphate end of a nucleotide to the 3´ hydroxyl end, accompanied by the hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA, DNA, and dinucleoside pyrophosphates.