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Thermo Scientific™ Pierce PolyHis Protein Interaction Pull-Down Kit purifies protein interaction complexes involving practically any user-supplied His-tagged fusion protein.

The PolyHis Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with recombinant proteins containing a histidine (6xHis) fusion tag. You provide the tagged fusion protein (bait) and cells expressing the putative protein interaction target (prey), and the pull-down kit supplies everything else: cell lysis buffer, microcentrifuge spin columns, cobalt-IMAC affinity resin (agarose beads), optimized binding and elution buffers, and a detailed protocol. This kit is designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.

Highlights:

• 6xHis pull-down – purifies protein interactors of any His-tagged fusion protein
• Complete kit – provides all components and detailed protocol for purifying protein:protein interactions
• No special equipment needed – uses common laboratory equipment and reagents (e.g., microcentrifuge)
• Convenient – microcentrifuge spin columns facilitate simple and efficient manipulation of cobalt IMAC agarose beads, including simple processing of multiple samples
• Flexible – instructions include protocols for bait and prey proteins from different sources

Includes:

Cobalt chelate agarose resin, lysis buffer, TBS pack, imidazole solution for elution, microcentrifuge spin columns and collection tubes

Recommended for:

Discover a new protein:protein interaction from a cell lysate; Confirm a putative interaction from a cell lysate or with a previously purified protein; Extract protein:protein interaction information from in vitro transcription/translation lysates