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Thermo Scientific™ Sulfo-EMCS (N-ε-maleimidocaproyl-oxysulfosuccinimide ester)
Thermo Scientific™ Pierce Sulfo-EMCS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 Ås).
Highlights:
- Water-soluble
- Cleavable at pH 8.5 using hydroxylamine and heat
- Lactose dehydrogenase retained 60% of its activity after reversible crosslinking
- Reactive groups: sulfo-NHS esters (homobifunctional)
- Reactive toward: amino groups