L00683-51
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High Affinity Ni-Charged Resin FF Prepacked Column
$100.05 Add to cart View Product DetailsGenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.
Cat. No. Product Name Volume supplied Resin content L00683-12 High Affinity Ni-charged Resin FF prepacked column (1mlx2) 1mlx2 prepacked column L00683-51 High Affinity Ni-charged Resin FF prepacked column (5mlx1) 5mlx1 L00683-55 High Affinity Ni-charged Resin FF prepacked column (5mlx5) 5mlx5 Storage
Store in 20% ethanol at 2°C to 8°C. Do not freeze.Characteristics
Binding capacity >40 mg of histidine-tagged protein /ml settled resin Matrix spherical 6% highly cross-linked agarose Average particle size 90 μm (45-165 μm) Storage solution 20% ethanol Storage and Stability 18 months when stored unopened at 2-8 °C Column compatibility
The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).Reagents Compatible
Denaturants Detergents Reducing agents Salts Others 6 M Gu·HCl 2% Triton X-100 20 mM β-ME 4 M MgCl2 50% glycerol 8 M Urea 2% Tween 20 1 mM DTT 5 mM CaCl2 20% ethanol 1% CHAPS 2 M NaCl 1 mM EDTA
