L00684-12
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Ni-IDA Resin FF Prepacked Column
$40.54 Add to cart View Product DetailsGenScript Ni-IDA Resin FF is a 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by three coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. Ni-IDA Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. Ni-IDA Resin FF Prepacked Column is an excellent choice for high performance purification of polyhistidine-tagged proteins.
Cat. No. Product Name Volume supplied Resin content L00684-12 Ni-IDA Resin FF prepacked column (1mlx2) 1mlx2 prepacked column L00684-51 Ni-IDA Resin FF prepacked column (5mlx1) 5mlx1 L00684-55 Ni-IDA Resin FF prepacked column (5mlx5) 5mlx5 Characteristics
Binding capacity >40 mg of histidine-tagged protein /ml settled resin Matrix spherical 6% highly cross-linked agarose Average particle size 90 μm (45-165 μm) Storage solution 20% ethanol Storage and Stability 18 months when stored unopened at 2-8 °C Column compatibility
The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).
