L00689-30
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GenCrispr sgRNA Screening Kit
$143.18 Add to cart View Product DetailsCas9 Nuclease NLS, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The design of single guide RNA (sgRNA) is dependent on the target region close to the PAM site. Even if you pick a target sequence that fulfills all of the described requirements, sgRNA specificity and activity is unpredictable. Therefore, it is often recommended that multiple, different sgRNAs be designed to target a gene of interest. The GenCrispr sgRNA Screening Kit provides a simple, reliable, and rapid method for assessing sgRNA efficiency before cell transduction, allowing you to identify the highly effective CRISPR sgRNA.
Components Amount 30-reaction kit 100-reaction kit GenCrispr Cas9 Nuclease 15 μL 50 μL 10X Reaction Buffer 100 μL 300 μL Positive Control sgRNA 3 μg 10 μg Positive Control Substrate 60 μL 200 μL RNase-free water 1 mL 1 mL
