RK20516

T4 RNA Ligase 2, is an ATP-dependent double-stranded RNA ligase (dsRNA ligase), also known as T4 Rnl2 (gp24.1), which can be used for intramolecular circularization and intermolecular linear ligation of double-stranded RNA. Unlike T4 RNA Ligase 1, T4 RNA Ligase 2 exhibits significantly higher activity in connecting gaps (nicks) in double-stranded RNA compared to connecting ends of single-stranded RNA. T4 RNA Ligase 2 is also applicable for intra- or inter-molecular ligation of the 3′ hydroxyl of RNA chains and the 5′ phosphate of DNA chains within double-stranded nucleic acids (such as RNA double strands, RNA/DNA hybrids, and/or DNA double strands). The enzyme requires an adjacent 5´ phosphate and 3´ OH for ligation. The enzyme can also ligate the 3´ OH of RNA to the 5´ phosphate of DNA in a double stranded structure.

T4 RNA Ligase 2, is an ATP-dependent double-stranded RNA ligase (dsRNA ligase), also known as T4 Rnl2 (gp24.1), which can be used for intramolecular circularization and intermolecular linear ligation of double-stranded RNA. Unlike T4 RNA Ligase 1, T4 RNA Ligase 2 exhibits significantly higher activity in connecting gaps (nicks) in double-stranded RNA compared to connecting ends of single-stranded RNA. T4 RNA Ligase 2 is also applicable for intra- or inter-molecular ligation of the 3′ hydroxyl of RNA chains and the 5′ phosphate of DNA chains within double-stranded nucleic acids (such as RNA double strands, RNA/DNA hybrids, and/or DNA double strands). The enzyme requires an adjacent 5´ phosphate and 3´ OH for ligation. The enzyme can also ligate the 3´ OH of RNA to the 5´ phosphate of DNA in a double stranded structure.