ABclonal Technology

application (WB, ELISA) & cross reactivity (Arabidopsis thaliana)

application (WB, ELISA) & cross reactivity (Human, Rat)

application (WB, ELISA) & cross reactivity (Human, Rat)

application (WB, ELISA) & cross reactivity (Mouse, Rat)

application (WB, ELISA) & cross reactivity (Mouse, Rat)

application (WB, ELISA) & cross reactivity (Mouse, Rat)

application (WB, ELISA) & cross reactivity (Mouse, Rat)

application (WB, ELISA) & cross reactivity (Mouse, Rat)

application (WB, ELISA) & cross reactivity (Mouse, Rat)

ABScript Full Length Reverse Transcriptase for Single Cell

ABScript Full Length Reverse Transcriptase for Single Cell

ABscript II Reverse transcriptase is a recombinant M-MuLV reverse transcriptase with low RNase H activity and enhanced thermal stability.Compared to wild-type M-MuLV,recombinant M-MuLV reverse transcriptase can synthesize first-strand cDNA at higher temperatures.The enzyme is active at up to 48℃ with higher specificity and cDNA yield.The kit provides two reverse transcription primers.The Oligo-dT primer [d(T)₂₃VN] forces the primer to anneal to the beginning of the polyA tail.The optimized random primer premix has low specificity,and all RNA,including mRNA,rRNA,and tRNA can be used as a reverse transcription template.This kit can amplify cDNA up to 10 kb.

ABScript II One Step SYBR Green RT-qPCR Kit is a special kit for one-step RT-qPCR reaction by using SYBR Green I chimeric fluorescence. The kit takes RNA as the template, uses gene-specific primers, and reverse transcription and PCR reaction can be carried out continuously in the same tube without additional pipe opening and pipetting operations, greatly improving the detection flux and effectively preventing contamination. This reaction system can detect the amplification products in real time, greatly improving the detection sensitivity, and omitting the electrophoresis step after PCR reaction, which is very suitable for the detection of RNA virus and other trace RNA. This product integrates the superiority of ABScript II Reverse Transcriptase and Taq DNA Polymerase, cooperate with optimized buffer system, with high amplification efficiency and high amplification specificity, one-step RT – qPCR reaction can be stable. In addition, all the enzymes used in the reaction are made into enzyme Mix, which is easier and more convenient to operate.

ABScript II One Step SYBR Green RT-qPCR Kit is a special kit for one-step RT-qPCR reaction by using SYBR Green I chimeric fluorescence. The kit takes RNA as the template, uses gene-specific primers, and reverse transcription and PCR reaction can be carried out continuously in the same tube without additional pipe opening and pipetting operations, greatly improving the detection flux and effectively preventing contamination. This reaction system can detect the amplification products in real time, greatly improving the detection sensitivity, and omitting the electrophoresis step after PCR reaction, which is very suitable for the detection of RNA virus and other trace RNA. This product integrates the superiority of ABScript II Reverse Transcriptase and Taq DNA Polymerase, cooperate with optimized buffer system, with high amplification efficiency and high amplification specificity, one-step RT – qPCR reaction can be stable. In addition, all the enzymes used in the reaction are made into enzyme Mix, which is easier and more convenient to operate.

ABScript III One Step RT-qPCR Probe Kit with UDG V5 is a universal kit for one step RT-qPCR reactions using the probe method. This kit uses RNA as the template, reverse transcription and PCR reactions can be performed continuously in the same tube, which improves the detection throughput. This kit introduces the dUTP/UDG anti-contamination system, heat-sensitive UDG can rapidly degrade U-containing contaminants at room temperature; and the heat-sensitive UDG is rapidly inactivated during reverse transcription at 50℃, which will not affect the efficiency and sensitivity of RT-qPCR. The reaction system can perform real-time detection of amplified products and improve the detection sensitivity, which is very suitable for the detection of trace RNA such as RNA viruses.

ABScript III One Step RT-qPCR Probe Kit with UDG V5 is a universal kit for one step RT-qPCR reactions using the probe method. This kit uses RNA as the template, reverse transcription and PCR reactions can be performed continuously in the same tube, which improves the detection throughput. This kit introduces the dUTP/UDG anti-contamination system, heat-sensitive UDG can rapidly degrade U-containing contaminants at room temperature; and the heat-sensitive UDG is rapidly inactivated during reverse transcription at 50℃, which will not affect the efficiency and sensitivity of RT-qPCR. The reaction system can perform real-time detection of amplified products and improve the detection sensitivity, which is very suitable for the detection of trace RNA such as RNA viruses.

ABScript III Reverse Transcriptase is a genetically engineered M-MuLV reverse transcriptase with reduced RNase H activity and improved thermal stability. Compared with wild-type M-MuLV, it can synthesize first-strand cDNA at higher temperature. Product still has features such as high activity at 50-55℃, higher specificity, higher yield, and the ability to synthesize cDNA up to 12 kb.

ABScript III Reverse Transcriptase is a genetically engineered M-MuLV reverse transcriptase with reduced RNase H activity and improved thermal stability. Compared with wild-type M-MuLV, it can synthesize first-strand cDNA at higher temperature. Product still has features such as high activity at 50-55℃, higher specificity, higher yield, and the ability to synthesize cDNA up to 12 kb.

ABScript III RT Master Mix for qPCR is developed based on ABScript II Reverse Transcriptase and suitable for two-step RT-qPCR detection. 5X ABScript III RT Mix contains all the reagents needed for reverse transcription reaction (ABScript III Reverse Transcriptase, RNase Inhibitor, RandomPrimer, Oligo dT Primer, dNTP and reaction buffer), and a reaction can be started simply by adding template RNA and Nuclease-free H₂O.
This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)₂₀VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance gene expression analysis.

ABScript III RT Master Mix for qPCR is developed based on ABScript II Reverse Transcriptase and suitable for two-step RT-qPCR detection. 5X ABScript III RT Mix contains all the reagents needed for reverse transcription reaction (ABScript III Reverse Transcriptase, RNase Inhibitor, RandomPrimer, Oligo dT Primer, dNTP and reaction buffer), and a reaction can be started simply by adding template RNA and Nuclease-free H₂O.
This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)₂₀VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance gene expression analysis.

ABScript III RT Master Mix for qPCR with gDNA Remover is developed based on ABScript III Reverse Transcriptase and suitable for two-step RT-qPCR detection. The 5X ABScript III RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H2O. The gDNA Remover Mix in this product can completely remove the genomic DNA remaining in the RNA template and make the qPCR results more accurate. The dsDNase is heat-sensitive and can be quickly and irreversibly inactivated under high temperature conditions. Therefore, it only needs one sample to be used to remove genomic DNA contamination and reverse transcription reactions in the same tube.
This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)₂₀VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance geneexpression analysis.

ABScript III RT Master Mix for qPCR with gDNA Remover is developed based on ABScript III Reverse Transcriptase and suitable for two-step RT-qPCR detection. The 5X ABScript III RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H2O. The gDNA Remover Mix in this product can completely remove the genomic DNA remaining in the RNA template and make the qPCR results more accurate. The dsDNase is heat-sensitive and can be quickly and irreversibly inactivated under high temperature conditions. Therefore, it only needs one sample to be used to remove genomic DNA contamination and reverse transcription reactions in the same tube.
This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)₂₀VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance geneexpression analysis.

ABScript Neo RT Master Mix for qPCR is an efficient and fast cDNA first-strand synthesis master mix, suitable for two-step RT-qPCR detection. The 4X ABScript Neo RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H₂O.
This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)20VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance gene expression analysis.

ABScript Neo RT Master Mix for qPCR with gDNA Remover is an efficient and fast cDNA first-strand synthesis master mix, suitable for two-step RT-qPCR detection. The 4X ABScript Neo RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H₂O. The gDNA Remover Mix in this product can completely remove the genomic DNA remaining in the RNA template and make the qPCR results more accurate. The dsDNase is heat-sensitive and can be quickly and irreversibly inactivated under high temperature conditions. Therefore, it only needs one sample to be used to remove genomic DNA contamination and reverse transcription reactions in the same tube.

ABScript Neo RT Master Mix for qPCR with gDNA Remover is an efficient and fast cDNA first-strand synthesis master mix, suitable for two-step RT-qPCR detection. The 4X ABScript Neo RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H₂O. The gDNA Remover Mix in this product can completely remove the genomic DNA remaining in the RNA template and make the qPCR results more accurate. The dsDNase is heat-sensitive and can be quickly and irreversibly inactivated under high temperature conditions. Therefore, it only needs one sample to be used to remove genomic DNA contamination and reverse transcription reactions in the same tube.

application (WB, ELISA) & cross reactivity (Mouse, Rat)

application (WB, ELISA) & cross reactivity (Mouse, Rat)

application (WB, ELISA) & cross reactivity (Human, Rat)

application (WB, ELISA) & cross reactivity (Human, Rat)

application (WB, ELISA) & cross reactivity (Mouse)

application (WB, ELISA) & cross reactivity (Mouse)

application (WB, IHC-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IHC-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IHC-P, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IHC-P, IF/ICC, ELISA) & cross reactivity (Human, Mouse, Rat)

application (IHC-P, ELISA) & cross reactivity (Human,Mouse,Rat)

application (IHC-P, ELISA) & cross reactivity (Human,Mouse,Rat)

application (WB, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IHC-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, IHC-P, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, ELISA) & cross reactivity (Mouse, Rat)

application (WB, ELISA) & cross reactivity (Mouse, Rat)

application (WB, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, ELISA) & cross reactivity (Human, Mouse, Rat)

application (WB, ELISA) & cross reactivity (Human, Mouse)

application (WB, ELISA) & cross reactivity (Human, Mouse)

application (WB, IHC-P, ELISA) & cross reactivity (Human, Mouse, Rat)