Thermo Scientific

Biosate Peptone is an animal origin (AO) mixed hydrolysate comprised of 65% pancreatic digest of casein and 35% yeast extract. This combination provides synergistic nutritional benefits that are not provided by the components alone, resulting in a unique nutritional profile. Customers have shown the combined use of these two peptones, when supplied as Biosate Peptone, improves toxin production with Clostridial species for vaccines, such as Clostridium botulinum and Clostridium perfringens.

Features:
• Hydrochloric acid hydrolysate of casein
• Animal origin nutritional supplement
• Milk protein rich in amino acid nitrogen

Applications of Biosate Peptone include:
• Intended for use as a nutritional supplement in vitamin assays, susceptibility testing, and other laboratory media, and microbial fermentation in which the high salt content will not interfere

• • Collagenase is a protease that cleaves the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Gly-Pro, which is found with high frequency in collagen

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• • It is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in connective tissues such as skin, tendon, blood vessels, and bone

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• • Collagenase disaggregation is suitable for the culture of human tumors, mouse kidney, human adult and fetal brain, and many other tissues including epithelium

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• • Collagenase is relatively gentle, dissociates well at physiological temperature and pH, and requires no mechanical agitation or special equipment

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• • Gibco™ Collagenase Type I is isolated from Clostridium histolyticum and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation, and organ perfusions

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• • Compared to other collagenase preparations, Gibco™ Collagenase Type I has average levels of collagenase, caseinase, clostripain, and tryptic activities, and is well-suited for the digestion of fat, adrenal, and liver cells or tissues

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• • Gibco™ Collagenase Type I activity is guaranteed to be greater than 125 units/mg

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• • Store at 2°C to 8°C, protected from light

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Difco Beef Extract is an animal origin peptone derived from an infusion of beef. This nutritional supplement provides a mixture of peptides, individual amino acids, nucleotides, organic acids, minerals, and vitamins. Provided as a paste, customers have used Difco Beef Extract in a variety of microbial fermentation cell lines, including Escherichia coli, Bacillus subtillis, Pasteurella multocida, and Streptococcus species to produce numerous animal health vaccines.

Features:
• An animal origin nutritional supplement
• A mixture of peptides, individual amino acids, nucleotides, organic acids, minerals, and vitamins

Applications of Difco Beef Extract include:
• Use in a variety of microbial fermentation cell lines
• Used to produce numerous animal health vaccines

Difco Phytone, ultra-filtered (UF), is an animal origin-free enzymatic digest of soy. Due to the naturally high concentrations of vitamins and carbohydrates in soy, Difco Phytone UF is a nutritious and excellent carbohydrate source. This product is an ultra-filtered version of Phytone Peptone, making it desirable for use with mammalian cell culture applications where reduced endotoxin levels are important.

Features:
• Ultra-filtered
• Animal origin-free nutritional supplement
• Excellent carbohydrate source due to the naturally high concentrations of vitamins and carbohydrates in soy

Applications of Difco Phytone, ultra-filtered (UF), include:
• Desirable for use with mammalian cell culture applications where reduced endotoxin levels are important
• Recommended for use in media for the cultivation of a wide variety of organisms, including fungi

Difco Soytone is an enzymatic digest of soy. With high concentrations of vitamins and carbohydrates uniquely present in soy, this supplement offers an alternative carbohydrate profile to yeast supplements for mammalian cell processes, such as CHO.

Features:
• An enzymatic digest of soy
• High concentrations of vitamins and carbohydrate

Applications of Difco Soytone include:
• An alternative carbohydrate profile to yeast supplements for mammalian cell processes, such as CHO

Difco Tryptone Peptone is an animal origin (AO) pancreatic digest of casein. In addition to providing a unique nutritional profile, it has a higher nitrogen content than our other casein products. Tryptone Peptone supports the growth of many different types of bacteria and fungi, including fastidious organisms and in toxin production. Customers have used this supplement in the development of animal health vaccines, such as Clostridial vaccines, and with human health Botox-like products produced from Clostridium botulinum.

Features:
• Animal origin, pancreatic digest of casein
• Primary nitrogen source in trypticase soy broth and agar
• Referenced in Official Methods of Analysis of AOAC International
• Supports the growth of many different types of bacteria and fungi

Difco Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells. Our yeast extracts are derived from primary grown baker’s yeast. These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications. Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation.

Difco Yeast Extract Low-Dusting (LD) was designed to limit the amount of dust generated compared to other spray-dried yeast extracts. Difco Yeast Extract UF is ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g.

Related yeast extract products:
• Bacto Yeast Extract—one of the most complete and versatile fermentation supplements available. It is an important ingredient for the microbiological assay of vitamins. Yeast extract is also of value in the assay of antibiotics. B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract.
• Gibco Yeast Extract—developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Bacto Yeast Extract, Technical—developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics

Difco Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells. Our yeast extracts are derived from primary grown baker’s yeast. These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications. Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation.

Difco Yeast Extract Low-Dusting (LD) was designed to limit the amount of dust generated compared to other spray-dried yeast extracts. Difco Yeast Extract UF is ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g.

Related yeast extract products:
• Bacto Yeast Extract—one of the most complete and versatile fermentation supplements available. It is an important ingredient for the microbiological assay of vitamins. Yeast extract is also of value in the assay of antibiotics. B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract.
• Gibco Yeast Extract—developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Bacto Yeast Extract, Technical—developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics

Sera for cell culture with robust cell lines
• Good value for basic research
• Endotoxin level: = 20 EU/mL (levels routinely = 10 EU/mL)
• Hemoglobin level: = 25 mg/dL
• Origin: Refer to CoA for specific origin (US, Canada, Mexico, Brazil, Australia, New Zealand)n

Gibco serum delivers:
• ISO 13485-certified, processed in FDA-registered facilities
• Triple filtered at 0.1 µm
• Gibco bottle is easier to use in hood, reduces risk of contamination, and helps you perform cell culture more consistently
• Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook

• Routinely used for attachment and maintenance of human embryonic stem cells (hESC)
• Human ESCs have specific needs for attachment and maintenance and some lots of basement membrane extract may not provide adequate support
• Geltrex™ hESC-qualified reduced growth factor basement membrane matrix has been tested for support of hESC growth and pluripotency, therefore minimizing the need to test multiple lots
• Consistent manufacturing processes help ensure a protein concentration averaging 15 mg/mL with very little variation from lot-to-lot

• • A water-soluble antibiotic originally purified from the bacterium Streptomyces kanamyceticus

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• • Kanamycin acts by binding to the 30S subunit of the bacterial ribosome and inhibiting protein synthesis in susceptible bacteria

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• • Gibco™ Kanamycin is effective against a wide variety of gram-negative and some gram-positive bacteria

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• • It is used for the prevention of bacterial contamination of cell cultures

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• • Provided as a 10 mg/mL stock solution, the recommended working concentration for cell culture is 100 µg/mL

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• • Manufactured at a cGMP compliant facility that is registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards

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• • Store at -5°C to -20°C

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For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Order Info

Shipping Condition: Room Temperature

Compliance

Manufactured at a cGMP compliant facility, which is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Phytone Peptone is an animal origin-free (AOF) enzymatic digest of soy. Due to the naturally high concentrations of vitamins and carbohydrates in soy, Phytone Peptone is a nutritious and excellent carbohydrate source. Customers have successfully used this supplement in CHO-based production of monoclonal antibodies for oncological and rheumatological therapeutics, as well as with many different microbial cell types to produce animal health vaccines for swine, such as Clostridial vaccines.

Features:
• Animal origin-free (AOF) supplement
• Enzymatic digests of soybean meal/flour
• Retains the high vitamin and high carbohydrate content of the soy plant tissue

Applications of Phytone Peptone include:
• It is an excellent plant peptone for the cultivation of fungi and fastidious types of bacteria, such as members of the Clostridium and Neisseria genera
• It has been used in mammalian cell culture applications due to its high carbohydrate content

RevitaCell™ Supplement (100X) has been optimized for use with pluripotent stem cells (PSCs), either as a post-thaw recovery solution to improve cell viability or in combination with Essential 8™ Medium to enhance single cell passaging applications. It can also be used for recovery of primary neurons, traditionally a challenging cell to efficiently recover. The significant improvement seen in these applications is due to the presence of a more specific ROCK inhibitor coupled with molecules containing antioxidant and free radical scavenger properties that minimize the impact of stress on the cells.

• • Enabling—provides maximum cell viability when performing single cell passaging

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• • Rapid cell recovery—high post-thaw cell viability and growth of pluripotent stem cells (passaged as clumps or single cells) and neurons

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• • Consistent—formulated using animal origin-free and chemically defined materials for quality and confidence
    
    Single cell passaging
    Use of the RevitaCellTM Supplement for 18-24 hours post-single cell passaging provides vast improvement in cell survival. Cells are shown to reach passaging confluency within 3-4 days post-seeding.
    
    Higher specificity
    Kinase profiling of pro-survival small molecules in RevitaCell™ Supplement shows higher specificity in comparison to traditional ROCK inhibitors (i.e., Y-27632 and Thiazovivin).
    
    Quicker post-thaw recovery
    PSCs recovered at 40,000 viable cells/cm² using media supplemented with RevitaCell™ Supplement for the first 24 hours post-thaw recovery followed by feeding with unsupplemented Essential 8™ Medium will typically reach 80% confluency in <5 days, significantly reducing the average post-thaw recovery time of most cryopreservation solutions. Neurite outgrowth of neurons is also significantly improved using RevitaCell™ Supplement.

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Starter Pak No. 1 features a combination of yeast- and soy-based peptones. Three of the products in this pack have been ultra-filtered to reduce endotoxin levels. The yeast products add a mixture of peptides, amino acids, carbohydrates (simple and complex), nucleosides, and vitamins to any medium formulation. Starter Pak No. 1 provides conveniently packaged, 100-g, non-GMP samples of these peptones that are best suited for use in human and animal health applications and contains:
• Difco TC Yeastolate UF and Bacto TC Yeastolate—these peptones are ideal for CHO-based applications of biotherapeutic monoclonal antibodies and recombinant proteins
• Difco Yeast Extract UF and Bacto Yeast Extract, Technical—these peptones support optimal growth of many microbial species for a variety of human and animal health vaccines
• Difco Phytone Supplement, UF—this enzymatic digest of soy is an excellent, nutritious source of carbohydrates and is used in mammalian cell culture. This peptone works well alone and when blended with yeast-based peptones.

Our Starter Paks provide conveniently packaged samples of the peptones most commonly used in mammalian cell culture and microbial fermentation bioproduction processes. Starter Paks are tailored for specific applications, including the production of monoclonal antibodies, recombinant proteins, and vaccines.

Starter Pak No. 2 contain a variety of animal origin and animal origin-free peptones that offer many essential nutrients needed for the production of human and animal vaccines. It provides conveniently packaged, 100-g, non-GMP samples of these peptones that are best suited for vaccine production and contains:
• Bacto Yeast Extract—this yeast has the highest level of carbohydrates of our yeast products and works well in a variety of human and animal health vaccines
• Phytone Peptone and Difco Soytone—both enzymatic digests of soy, these peptones also are a nutritious source of carbohydrates. These products work well in microbial fermentation processes as well as in mammalian cell culture processes such as CHO. Blending these soy peptones with yeast peptones has been shown to provide additional benefit to cultures.
• Bacto Proteose Peptone No. 2 and Bacto Proteose Peptone No 3—these enzymatic digests of porcine protein provide nutrition for fastidious microorganisms. Bacto Proteose Peptone No. 3 can replace serum in many applications and helps increase monoclonal antibody and recombinant protein production in CHO cells.
• Bacto Casamino Acids—this supplement has low salt and iron content, making it an excellent supplement for media formulations for which nitrogen requirements are minimal

Starter Pak No. 3 provides a variety of yeast and soy products, ideal for processes where an animal origin-free medium is preferred. It provides conveniently packaged, 100-g, non-GMP samples of these peptones that are best suited for human and animal vaccine production and contains:
• Bacto Yeast Extract and Gibco Yeast Extract—these peptones contain a mixture of peptides, amino acids, carbohydrates, and vitamins to support optimal growth in microbial species, and are ideal for the production of vaccines
• Bacto TC Yeastolate—this peptone is ideal for CHO-based applications of biotherapeutic monoclonal antibodies and recombinant proteins, as well as vaccine applications
• Phytone Peptone and Difco Soytone—these soy-based supplements provide a nutritious source of carbohydrates for successful use in microbial fermentation. Blending these soy peptones with yeast peptones has been shown to provide additional benefit to cultures.
• Bacto Malt Extract—a water-soluble portion of malted barley, this peptone also provides carbohydrates for a variety of microbial fermentation processes

Cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. Used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells.

• • Gentle on cells

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• • Room temperature-stable for 24 months

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• • Easy to use

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• • Animal origin-free

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• • Gentle on cells, purity increases specificity due to the action of a single enzyme and reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts

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• • Can be directly substituted for trypsin in existing protocols

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• • Dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS

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Energy efficient, less hazardous, sustainable packagingnnCell Culture, Mammalian Cell Culture

TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A549, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE™ formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, Gibco™ TrypLE™ Select is:

• Gentle on cells—maintains cell health for reproducible results
• Room-temperature stable—convenient to use
• Easy to use—substitutes directly into existing protocols, with no inactivation required
• Animal origin free (AOF)—pure, consistent, and effective

Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells. They are derived from primary grown baker’s yeast. These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications. Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation.

Related yeast extract products:
• Bacto Yeast Extract—one of the most complete and versatile fermentation supplements available. It is an important ingredient for the microbiological assay of vitamins. Yeast extract is also of value in the assay of antibiotics. B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract.
• Bacto Yeast Extract, Technical—developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics
• Gibco Yeast Extract—developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF—ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g.
• Difco Yeast Extract Low-Dusting (LD)—designed to limit the amount of dust generated compared to other spray-dried yeast extracts.

Description

Thermo Scientific™ Halt Protease Inhibitor Cocktails are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction procedures.

nHalt Protease Inhibitor Cocktails are 100X solutions containing optimized concentrations of six broad-spectrum protease inhibitors stabilized in high-quality dimethylsulfoxide (DMSO). The cocktails effectively inhibit serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. The identity and concentration of each component is fully disclosed, leaving no uncertainties about variables and compatibility with experiments. Regular and EDTA-free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis, respectively. The stable, concentrated liquid cocktails are easy to use, and the several different package sizes make Halt Protease Inhibitor Cocktails exceptionally convenient for either small-scale or large-scale uses. Highlights:

• • Better than tablets – the refrigerator-stable, 100X liquid format is more effective and easier to use than individual inhibitors or other formats; just pipette exactly the amount you need

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• • No proprietary ingredients – fully disclosed formulation contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A in DMSO

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• • Multiple package sizes – choose among several package sizes, including single-use microtubes that keep protease inhibitors fresh and reduce the risk of reagent contamination

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• • Two popular formulations – regular formulation includes a separate vial of EDTA solution for optional metalloprotease inhibition; EDTA-free formulation ensures compatibility with isoelectric focusing

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• • Lysis buffer compatible – use with Thermo Scientific Pierce™ Cell Lysis Buffers or nearly any other commercial or homemade detergent-based lysis reagent

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nIncludes:nnProtease inhibitor cocktails with EDTA include separate 0.5M EDTA solution.

Hank’s Balanced Salt Solution (HBSS) formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation. This HBSS is modified as follows: with glucose; without calcium magnesium, or phenol. Animal origin-free. The complete formulation is available.

• • Sustainable packaging

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Cell Culture, Mammalian Cell Culturen

Order Info

Shipping Condition: Room Temperature

Hank’s Balanced Salt Solution (HBSS) formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation. This HBSS is modified as follows: with glucose; without calcium magnesium, or phenol. Animal origin-free. The complete formulation is available.

• • Sustainable packaging

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Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale. Highlights:

• • High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin

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• • Versatile – purify proteins using native or denaturing conditions

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• • Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives

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• • Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges

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• • Cost-effective – reuse the same batch of resin at least five times

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• • Easy to use – pre-formulated buffers available for kit formats

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nAvailable Formats:n

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  • Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles

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• • Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns

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• • Purification Kits – complete kits in all three column sizes

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• • Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing

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• • Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin

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nIncludes:nnKits contain HisPur Ni-NTA Columns (store at 4°C)

Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin.nn nnThe specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.nnHisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.nnHighlights:n

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  • High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin

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• • Versatile – purify proteins using native or denaturing conditions

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• • Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives

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• • Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges

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• • Cost-effective – reuse the same batch of resin at least five times

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• • Easy to use – pre-formulated buffers available for kit formats

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nAvailable Formats:n

• n
  • Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles

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• • Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns

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• • Purification Kits – complete kits in all three column sizes

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• • Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing

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• • Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin

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This antibody recognizes an epitope at the C-terminus of alpha-2 helix and a turn on one of the underlying beta strands within the human HLA-A2 histocompatibility antigen.

nHLA-A2 (44 kDa) is the most frequent HLA-A allele in human ethnic populations. HLA-A, together with HLA-B and HLA-C, represent human HLA class I major histocompatibility (MHC) antigens. These intrinsic membrane glycoproteins are expressed on nucleated cells and noncovalently associate with an invariant beta2 microglobulin. They carry foreign determits important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence.

Human 4-1BB Receptor ELISA Kit

The Applied Biosystems™ Human ACTB (actin, beta) Endogenous Control (FAM™ Dye / MGB Probe, Non-Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other TaqMan™ Gene Expression Assays. Probe is labeled with 6FAM™ dye – MGB and the primers are not limited. Can be used for singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan™ Gene Expression Assays, Custom TaqMan™ Gene Expression Assays, and/or Custom TaqMan™ Primers and Probes.

Assay Details:
Gene Symbol: ACTB
RefSeq: NM_001101.2
Probe Exon Location:1
Amplicon Size: 171
Corresponding TaqMan Assay ID: Hs99999903_m1

TaqMan™ Endogenous Controls
Eliminate months of assay design, formulation, and testing by using TaqMan™ Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression
Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan™ Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use
All components of the TaqMan™ Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan™ Universal PCR Master Mix (with or without AmpErase™ UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering
We build each endogenous control using our proven 5′ nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:

• • A FAM™ dye-labeled TaqMan™ MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)

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• • A VIC™ dye-labeled TaqMan™ MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

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• • A VIC™ dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
    
    Choosing the Right Endogenous Control
    Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.
    
    Multiplex vs. Singleplex PCR
    All TaqMan™ Endogenous Controls that contain probes labeled with the VIC™ reporter dye are primer limited. This allows multiplexing of TaqMan™ Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan™ Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .
    
    For Research Use Only. Not for use in diagnostic procedures.

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Inhibin beta A (human) Recombinant Protein, RP-75637, from Invitrogen.

nActivins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Activin A mutations are associated with fibrodysplasia ossificans progressive. RP-75637 contains purified recombinant human Inhibin beta A protein with an N-terminal hexahistidine tag. RP-75637 may be used as a positive control in many immunological assays.

Human BD-1 ELISA Kit

Human BD-2 ELISA Kit

Human BD-3 ELISA Kit

Human Betacellulin ELISA Kit

Human CTACK (CCL27) ELISA Kit

CTRP5 Synthetic Peptide, PEP-0003, from Invitrogen.

nThis peptide corresponds to 15 amino acids near the center of human CTRP5PEP-0003 can be used as a blocking peptide with polyclonal antibody PA5-20153.

DR5 Synthetic Peptide, PEP-0021, from Invitrogen.

nDR5 (Apo2, TRAIL-R2, TRICK2, KILLER) is a recently identified death domain containing receptor for TRAIL, which mediates TRAIL induced apoptosis. DR5 is a member of the TNF-receptor superfamily, and contains an intracellular death domain. DR5 can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by DR5. Two transcript variants encoding different isoforms of DR5 and one non-coding transcript have been found. Overexpression of DR5 induces apoptosis and activates NF-kappa-B. DR5 is expressed in a number of cell types, and to particularly high levels in lymphocytes and spleen. Diseases associated with DR5 dysfunction include squamous cell carcinoma, and diffuse infiltrative lymphocytosis syndrome. This peptide corresponds to 20 amino acids near the carboxy terminus of human DR5.PEP-0021 can be used as a blocking peptide with polyclonal antibody PA5-19895.

Human EG-VEGF ELISA Kit

ELISA

Human FGF-basic ELISA Kit

Human Follistatin ELISA Kit

Human G-CSF ELISA Kit

The Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™ / MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye – MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan™ Gene Expression Assays, Custom TaqMan™ Gene Expression Assays, and/or Custom TaqMan™ Primers and Probes.

Assay Details:
Gene Symbol: GAPDH
RefSeq: NM_002046.3
Probe Exon Location:3
Amplicon Size: 122
Corresponding TaqMan Assay ID: Hs99999905_m1

TaqMan™ Endogenous Controls
Eliminate months of assay design, formulation, and testing by using TaqMan™ Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression
Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan™ Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use
All components of the TaqMan™ Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan™ Universal PCR Master Mix (with or without AmpErase™ UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering
We build each endogenous control using our proven 5′ nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:

• • A FAM™ dye-labeled TaqMan™ MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)

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• • A VIC™ dye-labeled TaqMan™ MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

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• • A VIC™ dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
    
    Choosing the Right Endogenous Control
    Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.
    
    Multiplex vs. Singleplex PCR
    All TaqMan™ Endogenous Controls that contain probes labeled with the VIC™ reporter dye are primer limited. This allows multiplexing of TaqMan™ Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan™ Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .
    
    For Research Use Only. Not for use in diagnostic procedures.

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ELISA

Luteinizing Hormone Releasing Hormone Synthetic Peptide, PEP-10933, from Invitrogen.

nPEP-10933 is a synthetic peptide suitable for use as a control in ELISA and Western blot applications.PEP-10933 has the amino acid sequence: Pyr-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.Store lyophilized product at -20 C. Upon reconstitution store at 4C for 2-7 days or aliquot and store at -20C. For long term storage add HSA or BSA to 0.1%. Avoid repeated freeze/thaw cycles.

Human GRO-β (CXCL2) ELISA Kit

Human Heregulinβ-1 ELISA Kit

ELISA

ELISA

Human IGF-BP1 ELISA Kit

ELISA