Protein Analysis

Kit Description

GenScript cAMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cAMP (Adenosine 3′,5′-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. cAMP is an important secondary messenger in signal transduction pathways that follows a number of extracellular signals. cAMP activates or inhibits various enzymes by promoting their phosphorylation or dephosphorylation. Its concentration is converted from adenosine triphosphate (ATP) via adenylyl cyclases (AC), and is inactivated by hydrolysis to 5′-AMP by the actions of phosphodiesterases.

The anti-IgG Capture Plate is pre-coated with a fixed amount of Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free cAMP or specimen and HRP-cAMP are added to the well, they compete in the solution to interact with the cAMP antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the cAMP amount present in the unknown samples can be calculated by transforming its absorbance value.

cAMP-peroxidase conjugate is a product most suitable for GenScript’s THE^TM Anti cAMP monoclonal antibody (Cat. No. A01509) and Rabbit Anti-cAMP polyclonal antibody (Cat. No. A00614) in immunoassay development for cAMP detection. HRP is conjugated to the C-8 position of cAMP.

Kit Description

GenScript cGMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cGMP (Guanosine 3′,5′-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. cGMP is an important secondary messenger in signal transduction pathways. It is a common regulator of ion channel conductance, glycogenolysis, and cellular apoptosis. cGMP is a cyclic nucleotide derived from guanosine triphosphate (GTP). The cellular production of cGMP is mediated by Guanylate cyclase (GC). It activates the cGMP-dependent protein kinases which in turn phosphorylate several downstream protein targets. cGMP has an effect on the regulation of cAMP levels by activating or inhibiting specific phosphodiesterases (PDEs).

The anti-IgG Capture Plate is pre-coated with fixed amount of Goat anti-rabbit IgG to capture Rabbit Anti-cGMP Polyclonal Antibody. When free cGMP or specimen and HRP-cGMP conjugate are added to the well, they compete in the solution to interact with the cGMP antibody captured on the plate. Other unbound molecules are removed by a wash step. The cGMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the amount of cGMP present in the unknown samples can be calculated by transforming its absorbance value.

cGMP-peroxidase conjugate is a product most suitable for GenScript’s THE^TM Anti cGMP monoclonal antibody (Cat. No. A01508) and Rabbit Anti-cGMP polyclonal antibody (Cat. No. A00615) in immunoassay development for cGMP detection. HRP is conjugated to the C-8 position of cGMP.

Each kit contains 2x 1L eBlot L1 PVDF Membrane Transfer Buffer (5X), 2x 15mL eBlot L1 PVDF Membrane Equilibration Buffer (10X), 2x 30pK eBlot L1 Transfer Sponge and 2x 15pK eBlot L1 PVDF Membrane. The kit is for 30x transfers with standard program.

Transfer Cassette

eBlot L1 is a highly efficient wet protein transfer system, which uses a patented technology developed by GenScript. eBlot L1 combines the high stability and efficiency of the traditional wet transfer with the rapidness and convenience of the semi-dry transfer system. The device allows the efficient transfer of small, medium as well as large molecular weight proteins within 15 minutes.

Each kit contains 2x 1L eBlot L1 NC Membrane Transfer Buffer (5X), 2x 15mL eBlot L1 NC Membrane Equilibration Buffer (10X) and 2x 30pk eBlot L1 Transfer Sponge. The kit is for 30x transfers with standard program.

eBlot L1 is a highly efficient wet protein transfer system, which uses a patented technology developed by GenScript. eBlot L1 combines the high stability and efficiency of the traditional wet transfer with the rapidness and convenience of the semi-dry transfer system. The device allows the efficient transfer of small, medium as well as large molecular weight proteins within 15 minutes.

Each kit contains 2x 1L eBlot L1 PVDF Membrane Transfer Buffer (5X), 2x 15mL eBlot L1 PVDF Equilibration Buffer (10X ) and 2x 30pK eBlot L1 Transfer Sponge. The kit is for 30x transfers with standard program.

eStain Filter Paper for eStain L1, eStain L1C, and eStain LG.

eZwest Cleaning Cassette for eZwest Lite (L00816)

Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, which bind to the Fc region of immunoglobulins, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L.

Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, which bind to the Fc region of immunoglobulins, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L.

Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, which bind to the Fc region of immunoglobulins, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L.

Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, which bind to the Fc region of immunoglobulins, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L.

Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, which bind to the Fc region of immunoglobulins, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L.

Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, which bind to the Fc region of immunoglobulins, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L.

Liquid container for eStain L1, eStain L1C, eStain LG，eBlot L1, and eBlot LG.

Membrane spacer for eZwest Lite (L00816)

Protein A affinity chromatography is widely used for antibody purification. However, the leakage of Protein A, though covalently conjugated to chromatography supports, posts a big problem for antibody based pharmaceutical industry where false results could be generated in immunological assays from contaminated Protein A.

GenScript Protein A ELISA Kit
is developed for accurate quantification of the leakage of native Protein A, recombinant Protein A variants and MabSelect SuRe ^TM
in the presence or absence of antibodies. The kit offers pharmaceutical users a sensitive assay to measure mini amount of Protein A in therapeutic antibody products. It also facilitates Protein A resin manufacturers in monitoring leaching characteristics of their resins under specific conditions.

Key Features:

• Accurate detection of 20 pg/ml Protein A and 50 pg/ml MabSelect SuRe ^TM
  in antibody preps
• Broad-range recongnition of native Protein A, recombinant Protein A variants and MabSelect SuRe
  ^TM
• Low level deviation – Intra-assay
  <5%, Inter-assay <10%
• High accuracy/recovery within 85-115%
• Ready-to-use ELISA plate with fast & simple samples preparation
• Time-saving protocol – complete assay in less than 2 hours
• Exceptional lot-to-lot consistency ensured by high-quality monoclonal antibodies
• Wide applications for the quantification of Protein A contaminant and leaching

Storage:

The kit is stable for 12 months if stored at 2-8 &#8451.
Do NOT freeze any component of the kit.

Tube for eStain L1, eStain L1C, eStain LG，eBlot L1, and eBlot LG.