1 each

Thermo Scientific™ Pierce Trifluoroacetic Acid (TFA) is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

nFor complex peptide separations, the key to success can be to vary selectivity. Varying mobile phase composition on the same column can change selectivity enough to resolve peptide that would otherwise overlap. The TFA concentration is usually specified as 0.1% for reverse-phase HPLC of peptides. For reproducible separations from run-to-run or from lab-to-lab, it is essential to make concentrations the same.

Thermo Scientific™ Pierce Trifluoroacetic Acid (TFA) is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

nFor complex peptide separations, the key to success can be to vary selectivity. Varying mobile phase composition on the same column can change selectivity enough to resolve peptide that would otherwise overlap. The TFA concentration is usually specified as 0.1% for reverse-phase HPLC of peptides. For reproducible separations from run-to-run or from lab-to-lab, it is essential to make concentrations the same.

Freeze cells in tubes from 1 to 5 mL using the Thermo Scientific™ Mr. Frosty™ Freezing Container. The simple-to-use system is designed to achieve a rate of cooling very close to -1°C/minute, the optimal rate for cell preservation. It holds 12 to 18 tubes, and a screw top lid secures samples inside in the event of accidental container tipping.nn nnPolycarbonate containers with blue high-density polyethylene closure. Gray high-density polyethylene vial holder, foam insert.

• • Holds 12 to 18 tubes and has profile height of 86 or 151mm (3-7/16 to 5-5/16 in.), which saves precious freezer space during the cooling process

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• • Screw top lid secures samples inside in the event of accidental container tipping

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• • Eliminates unreliable, improvised foam container devices

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• • Easy to use—requires only 100% isopropyl alcohol and a mechanical freezer

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• • No contact with the alcohol: no wicking, no label removal and no compromise of tube hand printing

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Ordering Information: Containers have 4.75 in. diameter (120mm) closure.

Holds 72 test tubes

Variable Dispenser used with Prep Reagent for making sample dilutions (0.5 – 2.0 mL).

• • Constructed from a single rigid piece of polypropylene in a 384-well format

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• • Designed to work with Applied Biosystems 384-well Real-Time PCR systems and thermal cyclers

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• • Frosted to minimize interfering fluorescence from wells of cycling block

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• • Screened to eliminate auto-fluorescent plates

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• • Unique design provides barrier to ambient air to help ensure well-to-well temperature uniformity

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• • Each reaction plate includes unique serialized, eight character number label (barcode) that is user-readable and machine-readable to prevent tracking errors

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• • Platform: 3130 Genetic Analyzer, 3130xl Genetic Analyzer, 3500 Dx Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Dx Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, 7900HT System, GeneAmp™ 9700, QuantSt

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PCR, PCR & Real-Time PCR, Real Time PCR (qPCR)

• • Delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms

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• • Provides best in class performance including the widest quantifiable range, in both single and duplex reactions, even with challenging targets

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• • TaqMan Fast Advanced Master Mix contains AmpliTaq Fast DNA Polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components

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• • Designed to provide performance equal to or better than standard master mixes

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• • Engineered to retain a high level of performance in preassembled reactions for up to 72 hours

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• • It is supplied at a 2X concentration

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• • Store at 4°C

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Features of the TaqMan Fast Advanced Master Mix include:

• • Best-in-class performance – superior sensitivity, accuracy, dynamic range, and specificity compared to standard mixes in standard mode

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• • Engineered for enhanced benchtop stability – stable at room temperature for up to 72 hours in preassembled reactions

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• • Optimized for multiplexing – validated for duplexing with exogenous and endogenous internal positive control assays

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• • Reduced run times – optimized for fast mode on fast instruments and fast cycling conditions on standard instruments
    

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• • Seamlessly transitions into experiments – validated with TaqMan assays for gene expression and microRNAs, and TaqMan array micro-fluidic cards
    

Order Info

Shipping Conditions: Dry Ice

• • Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe

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• • The IPC can be spiked into samples to distinguish true target negatives from PCR inhibition

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• • This kit allows users to amplify a low-copy target DNA in the same tube with the IPC

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• • Although the target and IPC DNAs may differ in initial copy number, the concentration of the IPC primers in the PCR reaction is limiting so that the amplification efficiency of the target reaction is not compromised

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• • Pre-designed primers and TaqMan™ probe eliminate assay design

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• • Rapid assay development guidelines can minimize optimization time Separate components provide flexibility in assay set-up

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• • Reagents may be stored at 2°C to 4°C if they are to be consumed within one month

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• • Otherwise, store at -20°C to -25°C

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Perform efficient, gentle lysis and extraction of E.coli and other bacterial cells with Thermo Scientific™ B-PER Bacterial Protein Extraction Reagents.nn nnThese easy-to-use cell lysis reagents are nonionic detergent solutions that effectively disrupt cells and solubilize native or recombinant proteins without denaturation. Certain formulations include DNase I and lysozyme enzyme supplements to improve yield of large molecular weight proteins that are difficult to purify. B-PER Reagents are compatible with the addition of standard protease inhibitors and with downstream protein purification methods, such as GST purification and His-tagged immobilized metal affinity chromatography (IMAC). Highlights:

• • Ready to use – B-PER Reagents provide one-step E.coli cell lysis by a mild, nonionic detergent (proprietary) in your choice of Tris or phosphate buffer formulations

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• • Fast and simple – just add B-PER Reagent to a bacterial pellet, shake for 10 minutes and recover soluble proteins after pelleting the cell debris

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• • Excellent yields – recover both soluble and insoluble recombinant protein from bacterial lysates and purify inclusion bodies to near-homogeneous levels

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• • Flexible – B-PER Reagents are suitable for any scale of protein extraction and are available in phosphate and 1X and 2X Tris formulations, with and without enzymes

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• • Compatible – completely compatible with addition of protease inhibitor cocktails, and resulting protein extract can be used in protein assays, typical affinity purification methods (e.g., GST, 6xHis) and other applications

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Convenient, ready-to-use formats:

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  • B-PER and B-PER II (2X) Reagents are free of enzymatic components

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• • B-PER Reagent (in phosphate buffer) is an amine-free formulation for direct compatibility with labeling and crosslinking

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• • B-PER with Enzymes and B-PER Direct with Enzymes include lysozyme and DNase I for improved lysis and recovery

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nIncludes:nnReagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.

BACK PANEL FOR TEMPLATE-TAMER

Like CFDA SE, CellTrace™ Oregon Green™ 488 (carboxy-DFFDA SE) should be a useful tool for following proliferating cells. This Oregon Green™ 488 probe passively diffuses into cells, where it is colorless and nonfluorescent until its acetate groups are removed by intracellular esterases to yield a highly fluorescent, amine-reactive dye. Upon reaction with intracellular amines, the probe forms Oregon Green™ 488 conjugates that are well-retained by cells. Unlike fluorescein conjugates, Oregon Green™ 488 conjugates exhibit bright, photostable green fluorescence that is independent of pH at typical cellular pH values (pH-6-8).

Analytical comb for MiniCuve electrophoresis tank.

Analytical comb for MiniCuve electrophoresis tank.

Analytical comb for MiniCuve electrophoresis tank.

Analytical comb for MiniCuve electrophoresis tank.

Analytical comb for MiniCuve electrophoresis tank.

Preparative comb for MiniCuve electrophoresis tank.

Preparative comb for MiniCuve electrophoresis tank.

Contains all of the consumables necessary to test liquid samples using MP Biomedicals’ Peroxide, Free Fatty Acid, Malonaldehyde, Alkenal, Percent Fat, and Total Glycerin Test Kits. Requires the use of one of MP Biomedicals’ Liquid Sample Instrument Platforms (Item Number 07LI2000 or 07LI2020).

MP Biomedicals’ Solid Sample Consumables Platform contains all of the consumables necessary to run solid samples using MP Biomedicals’ Peroxide, Free Fatty Acid, Malonaldehyde, Alkenal, Percent Fat, and Total Glycerin Test Kits. This Solid Sample Consumables Platform requires the use of one of MP Biomedicals’ Solid Instrument Platforms (Item Number 07SI2000 or 07SI2020). Each Solid Sample Consumables Platform contains the following items: printer paper, membranes, precision tissue wipers, solid glass beads, 15mL conical tubes, 10-100uL pipette tips, 200-1000uL pipette tips, 12mm culture tubes, 12mm culture tube caps, sharpie marker.

Gibco CTS OpTmizer T-Cell Expansion SFM supports the growth and expansion of human T lymphocytes. It is a complete serum-free, xeno-free medium consisting of CTS OpTmizer T-Cell Expansion Basal Medium and CTS OpTmizer T-Cell Expansion Supplement, which are mixed together prior to use.

CTS OpTmizer T-Cell Expansion SFM is available with and without phenol red. This version of CTS OpTmizer SFM lacks phenol red, and is available in both bottle and bag format.

Features of CTS OpTmizer T-Cell Expansion SFM include:
• Supports high-density T cell culture (>4 x 10⁶ CD3+ T-cells/mL)
• Supports T-cell activation using Dynabeads magnetic beads, soluble antibodies, and stimulatory antibody-presenting cell protocols
• Similar phenotype, function (e.g., cytokine secretion profile), and viability to T-cells cultured with conventional human AB serum-supplemented medium
• Supports a T-cell phenotype similar to human serum-supplemented medium
• Demonstrates enhanced efficacy and persistence of CART-19 cells when grown in medium supplemented with CTS Immune Cell Serum Replacement (ICSR)

Demonstrated functional performance
Cells grown in CTS OpTmizer T-Cell Expansion SFM with or without human serum demonstrate good cytokine production profiles in polyclonally activated T cells. CTS OpTmizer T-Cell Expansion SFM maintains the desired the CD62L+ central memory T-cell phenotype.

Applied Biosystems Custom TaqMan Gene Expression assays are the custom version of our predesigned TaqMan assays for quantitative real-time PCR. The Custom TaqMan Gene Expression assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3’ end.

Features include:
• Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
• Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
• Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
• Accurate—identify small fold-changes with high accuracy of quantitation
• Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific’s bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
• Sequences provided with every Custom TaqMan Gene Expression Assay you design and order

Approximate ship time
Made to order: 4–6 days in North America, 4–8 days in Europe

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.

Dispenser set needed for the Alkenal Test Kit. Includes one dispenser for each reagent (A, B, and Preparation Reagent) and one reagent holding rack.

Dispenser set needed for the Free Fatty Acid Test Kit. Includes one dispenser for each reagent (A, B, and Preparation Reagent) and one reagent holding rack.