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Methyl acetoacetate is used as a chemical reagent used in the synthesis of pharmaceuticals. It participates in the Biginelli reaction, forming molecules including dihydropyrimidinones.Methyl acetoacetate (MAA) is used for the synthesis of alpha-substituted aceto- acetic esters and cyclic compounds, e.g. pyrazole, pyrimidine and coumarin derivatives.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Methyl stearate is used as a nonionic surfactant, thereby enhancing the solubility of chemicals by dissociating aggregates and unfolding proteins. It is a fatty acid ester, which is used as an emulsifier and stabilizer.

nThis Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Increase filtration efficiency. Thermo Scientific™ Nalgene™ Rapid-Flow™ single use bottle tops feature the exclusive Rapid-Flow support column design, providing fast flow rates and high throughput. The last line of defense against cell culture contamination—Nalgene Rapid-Flow bottle tops are the best choice for the filtration of cell culture media, serum, additives and buffers.

• • Available in PES and SFCA membranes. Devices are color coded according to membrane.

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• • Devices feature the fastest flow rate and lowest clogging of comparable alternatives

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• • 0.1um PES available for mycoplasma protection

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• • 0.2um PES devices are stem cell and mouse embryonic assay (MEA) tested

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• • Non-pyrogenic and endotoxin free – Rapid-Flow filters meet the most rigorous testing standards for any vacuum filtration unit

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• • Available with Fit for Purpose Sterile documentation

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Photometric analyzer with pre-programmed protocols for use with all MP Biomedicals’ SafTest kits

Roll of thermal paper (2 1/4 in x 80 ft)

Alexa Fluor™ 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor™ 647 Streptavidin Conjugates:
• Alexa Fluor™ 647 streptavidin conjugate has Ex/Em maxima of ~ (650/668)
• Bright, photostable fluorescence
• High solubility in aqueous solutions
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

MP Magnetic Rack 24 is a novel tool designed for fast and easy extraction of nucleic acids using a magnetic method.

• Easy to Handle
• Strongest Magnetic Material
• Fast and Efficient Magnetic Separation
• Up to 24 Tubes 

MP Magnetic Rack 8 is a novel tool designed for fast and easy extraction of nucleic acids using a magnetic method. It is commonly used in combination with MagBeads FastDNA Kits from MP Biomedicals.

• Small, Easy to Handle 
• Strong Magnetic Material 
• Fast and Efficient Magnetic Separation 
• Up to 8 tubes of 1 to 5mL 

MP Magnetic Rack 96

Fully automated and integrated platform to purify nucleic acids of the highest yield and purity for downstream applications.

Guarantee excellent dispensing results and day-to-day performance with Thermo Scientific™ Multidrop™ Combi, 384 and DW Reagent Dispenser Accessories.

NeutrAvidin Protein, FITC conjugate, 31006, from Invitrogen., 31006, from Invitrogen. Species Reactivity: ; Applications: ELISA, Immunohistochemistry, In Situ Hybridization (ISH), Western Blot.

nNeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified and conjugated forms of NeutrAvidin Protein provide exceptional performance in Western blot, ELISA and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin. Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin-binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin binding proteins. The specific activity for biotin-binding is approximately 14ug/mg of protein, which is near the theoretical maximum activity.

Color: White; Sterility: Sterile; Lid: With Lid

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nEnsure consistent and reliable results using the high quality Thermo Scientific™ Nunc™ F96 MicroWell™ White Polystyrene Plate. White plates are optimized for robotics, plate readers and liquid handling systems and designed for key imaging characteristics.nn n

• Background, autoflorescence and crosstalk are improved when you use Nunc Black or White microplates

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• Black microplates are recommended for florescence measurements; with minimum back-scattered light and background fluorescence

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• White microplates are recommended for luminescence measurements; with maximum reflection and minimal autoluminescence

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• Working volume range: 50 to 250µL/well

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• Choice of Thermo Scientific™ Nunclon™ Delta surface for cell-based assays

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• Customized bar coding is available

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Enhance cell attachment, growth and differentiation with Thermo Scientific™ Nunc™ Cell Culture Treated EasYFlasks™. These polystyrene flasks receive the proprietary Nunclon™ Delta surface treatment to maximize adhesion for a broad range of cell types. The ergonomic design with angled neck and wide opening allows easy access to the growth surface by pipettes or cell scrapers.

nFeatures:n

• • Ergonomic design with a 1/3 turn to open and close the cap

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• • Low profile to maximize incubator space

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• • Volume graduations printed and molded on both sides

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• • Slightly angled side walls for complete viewing under a microscope

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• • Sterilized by gamma irradiation and certified to be nonpyrogenic

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• • Nunclon Delta Certification, including testing on four different cell types, is provided in every case.

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nFilter Caps:n

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  • Filter membrane provides effective barrier against micro-organisms.

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• • Well-defined, hydrophobic filter membrane offers continuous venting with consistent gas exchange and no lot-to-lot variation.

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• • Chemically robust membrane meets USP class VI standards and is produced without animal-derived substances.

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nSolid Caps:n

• • Solid caps feature a “Y” mark that allows visual verification of vent position.

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• • Any of the legs in the “Y” pointing upwards indicates that the cap is in venting position.

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• • Vent position can be easily distinguished when flasks are stacked in the incubator.

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Thermo Scientific™ Nunc MicroWell 96-Well Optical-Bottom Plates with Polymer Base have a solid polystyrene black or white upper structures with a polystyrene film at the bottom of the plate. The plates are optimized for fluorescence and luminescence in cell culture and microscopic applications.nn nnWhite or black polystyrene upper structure with polymer or coverglass bottom plates combine the optical clarity of virgin crystalline polystyrene with optimal surface for a wide range of HTS applications.

• • Background, autoflorescence and crosstalk are improved when you use Nunc Black or White microplates

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• • Black microplates are recommended for florescence measurements; with minimum back-scattered light and background fluorescence

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• • White microplates are recommended for luminescence measurements; with maximum reflection and minimal autoluminescence

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• • Available in black for fluorescence assays or white for luminescence and fluorescence assays; both with clear bottoms

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• • Polymer base plate

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• • Working range, 50-200µL/well

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• • Customized bar coding is available

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nOrdering Information:nnPart No. 152040, 152036, 152028 and 152037 is produced in a clean environment.

Outlet Tubing 0.5 – 2.5M Dispenser

Description

nPierce Protease Inhibitor Tablets protect proteins from proteolytic degradation through inhibition of the serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. The tablets contain AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A. Stable for storage at 4°C for up to 12 months, each mini or large tablet tablet is sufficient for 10mL or 50mL of solution, respectively, and is suitable for reagent preparation prior to protein extraction from tissue and cultured cells.

Highlights:

• • Improved formulation&mdsashtablets dissolve quickly into a clear solution

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• • Economical – more cost-effective than other commercially available formulations for equivalent volumes

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• • Convenient&mdsashready-to-use, fully disclosed, broad-spectrum formulations

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• • Compatible&mdsashuse directly with Thermo Scientific™ Pierce™ BCA Assays

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• • EDTA-free- formulated without EDTA, a metalloproteinase inhibitor (only for EDTA-free formulations)

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Thermo Scientific™ RIPA Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.nn nnThis RIPA buffer effectively lyses and extracts protein from cultured mammalian cells, including plated cells and pelleted suspension cells. The popular reagent enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, the Thermo Scientific BCA Protein Assay, immunoassays and protein purification. Inhibitors such as Thermo Scientific Halt Protease Inhibitor Cocktail (Part No. 78430) and Halt Phosphatase Inhibitor Cocktail (Part No. 78420) are also compatible with this RIPA buffer formulation and can be added before use to prevent proteolysis and maintain protein phosphorylation.

Contains all of the instrumentation necessary to test liquid samples using MP Biomedicals’ Free Fatty Acid, Malonaldehyde, Percent Fat, and Total Glycerin Test Kits. Requires the use of MP Biomedicals’ Liquid Sample Consumables Platform (Item Number 07LC2000).

MP Biomedicals’ Solid Sample Instrument Platform contains all of the instrumentation necessary to run solid samples using MP Biomedicals’ Free Fatty Acid, Malonaldehyde, Percent Fat, and Total Glycerin Test Kits. This Solid Sample Instrument Platform requires the use of MP Biomedicals’ Solid Consumables Platform (Item Number 07SC2000).
Each Solid Sample Instrument Platform contains the following items: timer, two block dri-bath 110V, 12.75mm dri-block insert, vortex 110V, 13mm test tube rack, 10-100µL pipette PDP, vacuum pump assembly 110V, acrylic base, 100-1000µL pipette PDP, 15mm dri-block insert, 20mm test tube rack, SafTest analyzer, O-ring for vacuum pump assembly.

Contains all of the instrumentation necessary to test liquid samples using MP Biomedicals’ Peroxide or Alkenal Test Kit. Requires the use of MP Biomedicals’ Liquid Sample Consumables Platform (Item Number 07LC2000).

For use in conjunction with the Solid Sample Consumables Platform and the Peroxide or Alkenal Test Kit.

Porous Plate TransDNA for Vacuum blotter.

Pipettes well suited for liquids with high densities, viscosities or vapor pressures. To be used with Positive displacement pipette tips, 10-100 µL (catalog number 07DP1035)

Pipettes well suited for liquids with high densities, viscosities or vapor pressures. To be used with Positive displacement pipette tips, 100-1000 µL (catalog number 07DP1038)

Thermo Scientific™ QuantaBlu Fluorogenic Peroxidase Substrate is a soluble fluorogenic (chemifluorescent) substrate for detection of peroxidase activity in ELISA methodnnIn the presence of active peroxidase the working solution of QuantaBlu Substrate produces a blue fluorescent product that can be quantitated by fluorometry in microplates or cuvettes. Fluorometric-based detection has a large dynamic range, which overcomes the limitations of using spectrophotometry to measure colorimetric substrates. The blue fluorescent product is not sensitive to light and does not photobleach. QuantaBlu Fluorogenic Peroxidase Substrate is stable, highly sensitive, produces high signal-to-noise ratios and allows for a broad dynamic detection range of peroxidase activity. This substrate is also flexible and can be used for stopped, nonstopped and kinetic assays. Incubation for stopped and nonstopped assays can be performed between 5 and 90 minutes at either room temperature (RT) or 37°C. The QuantaBlu NS/K Substrate formulation can be integrated into robotic-based assays and is designed for assays that do not need to be stopped.