1 each

Perform quick protein runs with the economical and simple, yet rugged Thermo Scientific™ Owl™ P81 Single-Sided Vertical Electrophoresis System.

Features:

• Upper buffer chamber extends the length of the gel to distribute heat evenly across the entire gel surface
• Side clamps press glass plates against silicone gasket to provide uniform pressure and form a leakproof seal resulting in flat, even banding patterns
• Notched alumina ceramic plate (included) may be used in front of notched glass for cooler, faster runs

Includes:

• Upper and lower buffer chambers
• Thermo Scientific™ SuperSafe™ lid with attached power supply leads
• Blank glass plate
• Notched glass plate
• Notched alumina plate
• Two combs (10 well, 0.8mm thick)
• Spacer set (0.8mm thick)

Thermo Scientific™ Pierce BCA Protein Assay Kit remains one of the most popular protein quantitation methods worldwide.

nn

n

• • Colorimetric – estimate visually or measure with a standard spectrophotometer or plate reader (562nm)

n

• • Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods

n

• • Compatible – unaffected by typical concentrations of most ionic and nonionic detergents

n

• • Moderately fast – much easier and four times faster than the classical Lowry method

n

• • High linearity – linear working range for BSA equals 20 to 2000µg/mL

n

• • Sensitive – detect down to 5µg/mL with the enhanced protocol

n

• • Studying protein:protein interactions

n

• • Measuring column fractions after affinity chromatography

n

• • Estimating percent recovery of membrane proteins from cell extracts

n

• • High-throughput screening of fusion proteins

Thermo Scientific™ Pierce Biotinylated Protein Interaction Pull-Down Kit contains the necessary components to capture and purify interactors of a biotin-labeled protein or ligand.

The kit makes it possible to perform an efficient pull-down assay with high protein recovery and reproducibility. This protocol uses immobilized streptavidin to capture a biotinylated protein and a spin column format that enables complete gel retention during each capture, wash and elution step. When the biotinylated bait protein is immobilized, it can withstand stringent washes and remains bound during prey protein elution, which results in an isolated prey protein without bait protein contamination. This technique is ideal when using native or recombinant proteins that are not expressed with a fusion tag. Additionally, prey protein is isolated from the immobilized bait protein using native binding conditions and nondenaturing elution conditions. The Biotinylated Protein Interaction Pull-Down Kit is designed to teach the method to first-time users and to increase ease-of-use and convenience for experienced researchers.

Highlights:

• Biotin pull-down – purifies protein interactors of any biotinylated protein or biotin-tagged ligand
• Complete kit – provides all components and detailed protocol for purifying protein:protein interactions
• No special equipment needed – uses common laboratory equipment and reagents (e.g., microcentrifuge)
• Spin column efficiency – microcentrifuge spin columns facilitate microcentrifuge spin columns facilitate simple and efficient manipulation of streptavidin agarose beads, including simple processing of multiple samples

Includes:

Streptavidin agarose resin, TBS pack, biotin blocking buffer, wash buffer, elution buffer, spin columns and collection tubes

Recommended for:

Discover a new protein interaction complex using a biotinylated protein as the bait to capture a putative binding partner from a cell lysate or other sample; Confirm expression of a known protein interaction target in a cell lysate; Determine the ability of two purified proteins to interact (bind) in various buffer conditions; Extract protein:protein interaction information from in vitro transcription/ translation lysates

Thermo Scientific™ Pierce Disposable Plastic Columns are empty columns with matching frits and caps for use in a variety of resin-based affinity or desalting techniques.

These bulk packages of empty columns are economical and convenient devices for packing affinity purification supports and desalting resins for gravity-flow procedures. Three columns sizes are offered, suitable for procedures requiring 0.5 to 10mL resin-bed volumes. The plastic columns are supplied with matching pairs of polyethylene discs and polypropylene caps (top and bottom). The columns are economically-priced for single use and sufficiently durable to prepare and store columns for multiple uses.

Highlights:

• For bench top gravity-flow affinity purification and size exclusion chromatography
• Supplied complete with porous polyethylene discs, stoppers and end caps
• Columns can be pre-packed and stored until needed
• Compatible with most types of aqueous buffer eluents commonly used in chromatography
• Plastic funnels can be attached to expand the buffer reservoir for increased flow rates

Includes:

Trial pack contains two columns of each available size (six columns total), matching column accessories (discs and caps), and one funnel (column reservoir extender)

Thermo Scientific™ Pierce Disposable Plastic Columns are empty columns with matching frits and caps for use in a variety of resin-based affinity or desalting techniques.

These bulk packages of empty columns are economical and convenient devices for packing affinity purification supports and desalting resins for gravity-flow procedures. Three columns sizes are offered, suitable for procedures requiring 0.5 to 10mL resin-bed volumes. The plastic columns are supplied with matching pairs of polyethylene discs and polypropylene caps (top and bottom). The columns are economically-priced for single use and sufficiently durable to prepare and store columns for multiple uses.

Highlights:

• For bench top gravity-flow affinity purification and size exclusion chromatography
• Supplied complete with porous polyethylene discs, stoppers and end caps
• Columns can be pre-packed and stored until needed
• Compatible with most types of aqueous buffer eluents commonly used in chromatography
• Plastic funnels can be attached to expand the buffer reservoir for increased flow rates

Includes:

Trial pack contains two columns of each available size (six columns total), matching column accessories (discs and caps), and one funnel (column reservoir extender)

Thermo Scientific™ Pierce Heptafluorobutyric Acid (HFBA) is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

Highlights:

• Typical purity is 99.7% by GC; <0.1% water
• Sequencing reagent for classical and automated Edman degradation of peptides and proteins
• Density: 1.645
• B.P. 120°C
• Packaged under nitrogen in amber glass ampules or bottles
• Clear, colorless liquid

Ordering Information:

X in the ordering table indicates that hazardous shipping charges apply

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.

nTMB (3,3′,5,5′ tetramethylbenzidine) is a chromagen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) with major absorbances at 370 and 652nm. The color then changes to yellow (measured at 450nm) upon addition of sulfuric or phosphoric acid to stop the reaction. TMB is very sensitive and more quickly oxidized than other HRP substrates, resulting in faster color development. Unlike other commercially available substrates, these products contain no DMF or DMSO. There are three formulations that differ primarily in their sensitivities. Slow TMB is intermediate in sensitivity, ideal for kinetic readings. The sensitivity of the Turbo TMB compares to that of OPD used at approximately 1mg/mL. Ultra TMB-ELISA produces the highest signal-to-noise ratio and sensitivity in the picogram range.nnHighlights:n

• n
  • Ready-to-use solutions

n

• • No hydrogen peroxide required

n

• • No filtering required

n

• • Noncarcinogenic

n

• • Various sensitivities to suit any assay

n

n1-Step TMB Substratesn

• n
  • Ready-to-use, one-component substrates

n

• • Do not require hydrogen peroxide

n

• • Noncarcinogenic

n

• • Do not contain DMF or DMSO

n

• • Available in three formulations that vary by sensitivity

n

nTMB Substrate Kitn

• n
  • More sensitive than OPD in endpoint ELISAs

n

• • Easy to use

n

• • Provides results in seconds

n

nIncludes:nnTMB Substrate Kit (PI-34021): Peroxidase substrate, 200mL; Peroxide solution, 200mL

Reduce the amount of reagents used in the lab with Thermo Scientific™ Plates and Modules with Covalent Binding Surfaces.

Covalent Surfaces (Thermo Scientific™ CovaLink™ and Immobilizer Amino)

• • Minimal leaching

n

• • Withstands vigorous washing

n

• • Coating with lower amounts of reagent may be possible

n

• • Control of orientation

n

Help ensure measurable Class 10 air quality performance with Thermo Scientific™ Replacement HEPA Filters and Cleaning Kits.

HEPA Filters

• • 99.97% filtering at 0.3µm

n

• • Compatible with all Thermo Scientific™ Forma™ Series II 3110, Thermo Scientific™ Steri-Cycle™, and Forma™ 310 Series (with HEPA filtration system option) incubators

n

• • Main chamber filter available as a stand-alone or packaged in a group with the other chamber in-line filters

n

Decontamination Kits

• n
  • Consists of filters for difficult-to-clean chambers and gaskets (except door) and tubing

n

• • Reduces downtime and labor spent on decontamination

n

Carry out longer, fatigue-free pipetting with the Thermo Scientific™ S1 Pipette Fillers. These instruments are ergonomically designed and offer simple, efficient pipetting performance, as well as maximum pipetting comfort. Lightweight and cordless, they feature a heavy-duty rechargeable lithium-ion battery that allows approximately three times longer operation between charges than typical units. For use with graduated and volumetric glass and plastic serological pipets. Monitor Performance

• • Large, backlit LCD display provides visual confirmation of remaining battery charge and speed settings

n

nControl Speedn

• n
  • Separate aspirate and dispense speed controls provide precise control over pipetting speeds and reduce risk of overpipetting with smaller pipets

n

• • Choose from eight speeds with a simple “+” or “-” to adjust pipetting speed which is displayed on the LCD

n

nReady When You Need Itn

• n
  • Rechargeable lithium-ion battery offers long runtime per charge while eliminating battery memory problems

n

• • Battery has rapid charge rate of 80% in one hour and delivers up to 15 hours of continuous operation before recharge

n

n

Warranty and Services

n

2-years with web registration

Carry out longer, fatigue-free pipetting with the Thermo Scientific™ S1 Pipette Fillers. These instruments are ergonomically designed and offer simple, efficient pipetting performance, as well as maximum pipetting comfort. Lightweight and cordless, they feature a heavy-duty rechargeable lithium-ion battery that allows approximately three times longer operation between charges than typical units. For use with graduated and volumetric glass and plastic serological pipets.

Thermo Scientific™ SuperBlock Blocking Buffers are optimized PBS and TBS solutions containing a protein that provides incredibly fast and effective blocking for ELISA, immunohistochemistry and Western blotting applications.

nBlocking microplates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.nnHighlights:n

• • Fast – block membranes in 5 to 10 minutes and ELISA plates in 2 minutes

• • Flexible – guaranteed to be biotin-free for use with streptavidin system

• • Convenience – available in multiple buffer formats (in PBS or TBS with or without Tween™-20 detergent) and package sizes (1L, 5L and dry-blend pouches)

• • Low background – the non-serum protein solution yields a high signal-to-noise ratio

• • Stable – store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Thermo Scientific™ SuperSignal West Femto Substrate is an ultra-sensitive enhanced chemiluminescent substrate (ECL substrate) for detection of peroxidase activity from HRP-conjugated antibodies and probes in Western blotting.

nSuperSignal West Femto Substrate Maximum Sensitivity Substrate is an ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme. The substrate kit enables detection of low femtogram (that’s mid-zeptomole!) amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are small to be seen with typical ECL substrates.nnHighlights:n

• n
  • ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

n

• • Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes

n

• • Quantitative – produces quantitative signal that is measurable over two orders of magnitude

n

• • Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks)

n

• • Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system

n

• • Excellent signal duration – 8 hours of usable light-output when conditions are optimized

n

• • Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature

n

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility. Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• n
  • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

nCompatible with:nnLids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility.Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• n
  • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

nCompatible with:nnLids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility. Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• n
  • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

nCompatible with:nnLids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility. Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• n
  • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

nCompatible with:nnLids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility.

Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• n
  • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

nCompatible with:nnLids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility.

Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

Compatible with: Lids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Thermo Scientific™ Zeba Spin Desalting Columns (7K MWCO) contain a size-exclusion chromatography resin that provides excellent protein desalting performance in a centrifuge format for 2µL to 4mL samples.

nThese specially designed polypropylene centrifuge columns are pre-packed with high-performance Zeba Desalting Resin to facilitate rapid protein desalting with high recovery. The resin effectively traps salts and other small sample components but does not impede proteins and other macromolecules, which pass directly into the centrifuge collection tube. These particular columns use Zeba Resin having a molecular-weight cutoff (MWCO) rating equal to 7K; this ensures that proteins larger than 7000 MW will be recovered with high efficiency.nnThe easy-to-use spin column format dramatically improves results over standard drip-column methodologies. This system eliminates the need to wait for samples to emerge by gravity flow and to monitor fractions for protein recovery. Samples are processed in these desalting columns using a standard centrifuge or microcentrifuge. No chromatography hardware or column preparation steps are required. Multiple samples can be processed in less than 10 minutes. Zeba Spin Desalting Columns are available in five different column sizes, and these provide trouble-free desalting and buffer exchange for sample volumes ranging from 2µL to 4mL

Highlights:

• • Fast centrifuge format – resin and columns are optimized for rapid processing by centrifugation with standard collection tubes and rotors

• • Replaces drip-column methods – no need to collect and screen slowly emerging protein fractions

• • Exceptional protein yields – recover greater than 95% in typical applications with minimal dilution

• • Five convenient sizes – columns with 75µL to 10mL of Zeba Resin provide desalting matched for sample volumes between 2µL and 4mL

• • 7K MWCO – resin is rated to recover proteins larger than 7000 daltons and retain (remove) solutes smaller than 1000 MW

Requires:nnMicrocentrifuge and microcentrifuge collection tubes (for 75µL and 0.5mL columns) or centrifuge and collection tubes (15mL tubes for 2 and 5mL columns; 50mL tubes for 10mL columns)

User-friendly timer that displays three different countdown/alarm times simultaneously.

The MP Bio Transilluminator offers highly sensitive detection of double-stranded nucleic acids that have been labeled with fluorescent dyes such as ethidium bromide or acridine orange.

The MP Bio Transilluminator offers two wave-length for different applications. For the detection of double-stranded and single-stranded ethidium bromide/acridine orange stained nucleic acids..

The MP Bio Transilluminator offers three wave-length for different applications. For the detection of double-stranded and single-stranded ethidium bromide/acridine orange stained nucleic acids..

The MP Bio Transilluminator offers the researcher uniform and intense sources of ultraviolet light (radiation) in a compact package. The 302nm UV back-illumination provides a highly sensitive method to detect double-stranded nucleic acids that have been labeled with fluorescent dyes such as ethidium bromide or acridine orange. Single stranded nucleic acids may be detected, but with a lower excitation wavelength more sensitive for nucleic acid visualization than the 365nm model.

• • Each drop of beads contains both a positive population that will capture any mouse, rat or hamster antibody and a negative population that will not react with antibody

n

• • This bimodal distribution can be used for single-color compensation controls in multicolor flow cytometry experiments

n

• • Compatible with all fluorochromes excited by an ultraviolet (355nm) or violet (405nm) laser

n

• • Should be used with standard staining buffers which contain PBS or HBSS, protein such as BSA or FBS, and sodium azide. No other additives should be used.

n

• • Binds the spherical particles for use as a single-color compensation controls.

n

• • Binds with mouse, rat and hamster antibodies, but also bind with rabbit and recombinant human antibodies.

n

• • Designed for use in compensation with all fluorochromes excited by ultraviolet (355 nm) violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), and red (633-640 nm) lasers.

n

• • Compensate in a manner similar to cells when used with Brilliant Violet 785/786-, Brilliant Violet 711-, Super Bright 780- and Super Bright 702-conjugated antibodies.

n

• • Multispecies compatibility—bind with mouse, rat, hamster, rabbit and recombinant human antibodies

n

• • Improved fluorophore compatibility

n

• • Each drop of beads contains two populations: a positive population that bind with any mouse, rat, hamster, rabbit or recombinant human antibody and a negative population that will not bind with antibody.

n

• • When a fluorochrome-conjugated antibody is added to the beads, both positive and negative populations are observed.

n

• • This bimodal distribution can be used for single-color compensation controls to accurately setup instrumentation for multicolor flow cytometry experiments.

n

• • UltraPure DNase/RNase-Free Distilled Water is 0.1-µm membrane-filtered and tested for DNase and RNase activity

n

• • Performance and quality testing: No DNase, RNase, or protease activity detected. Our distilled water system is routinely monitored for compliance with current USP monograph test requirements for Water for Injection (WFI)

n

• • Sustainable packaging

n

Universal Repipet II Dispenser

UV BULB FOR TEMPLATE-TAMER

Long life UV tubes designed for MP Bio Transilluminators; Portable lamps or Template Tammer

The Vacuum Blotter for quick and efficient transfer of DNA or RNA onto suitable membrane such as Positive Nylon membrane. Transfer efficiency close to 100% in less than one hour with low transfer solution consumption. Large working area 28 x 28 cm.

Vitamin E acetate is used as a fortifying agent and dietary supplement in food. It is used in dermatological products such as skin creams. It is an alternative to tocopherol and provides protection against the sun’s ultraviolet rays.

nThis Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Used in conductometric titration, Synthesis of nanomaterials. Mostly it is used as solvent in industries and in all research fields.

nThis Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

WHITE LIGHT BULB FOR T TAMER

Rapidly desalt protein and biological samples for manual or automated liquid chromatography (LC) with Thermo Scientific Zeba Spin Desalting columns, plates, and cartridges, which enable =95% retention of salts and other small molecular contaminants (<1,000 Da). Zeba Spin Desalting products contain a proprietary high-performance size-exclusion chromatography resin that enables excellent protein desalting and recovery of 7K molecular weight cutoff (MWCO) molecules using a centrifuge format.

nZeba Spin Desalting columns, plates, and chromatography cartridge fittings are convenient, ready-to-use devices compatible with major automated liquid-chromatography systems or for manual syringe processing. The polypropylene columns have a 7K MWCO and contain a proprietary high-performance size-exclusion chromatography resin (Zeba High-Performance Resin) that offers excellent protein desalting and recovery in a centrifuge format. When using Zeba Spin Desalting Columns, even very dilute (25 µg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules (<1000 MW), and good recovery of proteins and other macromolecules (>7000 MW).

Similarly, Zeba Desalting plates (7K MWCO) are polypropylene devices that contain Zeba High-Performance Resin and provide excellent protein desalting performance, with high recovery of proteins >7000 Da in a centrifuge format for small sample volumes. The plates enable fast, consistent, well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations. Zeba 96-well spin desalting plates require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100 µL) in just five minutes, offering rapid and exceptional protein desalting.

The Zeba Desalting Chromatography Cartridges contain Zeba High-Performance Resin for desalting or buffer exchange applications. This resin is ideal for removing low molecular weight compounds including salts, fluorescent dyes, biotin, and other small labeling reagents. Protein samples can be processed with unsurpassed protein recovery and 95% retention of salts and other small molecular contaminants (<1,000 Da). Zeba Desalting cartridges provide fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses.

Benefits of Zeba Spin Desalting columns, plates, and cartridges include:
• High recovery—low-binding resin maximizes protein recovery
• Fast—no fraction screening or waiting for protein to emerge by gravity flow
• Economical—great performance at lower cost than other commercially available cartridges
• Easy-to-use—no cumbersome column preparation or equilibration
• Flexible—available in spin columns, filter spin plates and cartridges for a range of needs

Available formats
• Spin columns—75 µL, 0.5 mL (microcentrifuge), 2 mL, 5 mL, 10 mL columns
• 96-well spin plates—pre-dispensed 96-well filter plates, compatible with centrifugation for manual or automated purification
• Chromatography cartridges—compatible with automated liquid chromatography systems and with syringe processing

Rapidly desalt protein and biological samples for manual or automated liquid chromatography (LC) with Thermo Scientific Zeba Spin Desalting columns, plates, and cartridges, which enable =95% retention of salts and other small molecular contaminants (<1,000 Da). Zeba Spin Desalting products contain a proprietary high-performance size-exclusion chromatography resin that enables excellent protein desalting and recovery of 7K molecular weight cutoff (MWCO) molecules using a centrifuge format.

nZeba Spin Desalting columns, plates, and chromatography cartridge fittings are convenient, ready-to-use devices compatible with major automated liquid-chromatography systems or for manual syringe processing. The polypropylene columns have a 7K MWCO and contain a proprietary high-performance size-exclusion chromatography resin (Zeba High-Performance Resin) that offers excellent protein desalting and recovery in a centrifuge format. When using Zeba Spin Desalting Columns, even very dilute (25 µg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules (<1000 MW), and good recovery of proteins and other macromolecules (>7000 MW).

Similarly, Zeba Desalting plates (7K MWCO) are polypropylene devices that contain Zeba High-Performance Resin and provide excellent protein desalting performance, with high recovery of proteins >7000 Da in a centrifuge format for small sample volumes. The plates enable fast, consistent, well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations. Zeba 96-well spin desalting plates require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100 µL) in just five minutes, offering rapid and exceptional protein desalting.

The Zeba Desalting Chromatography Cartridges contain Zeba High-Performance Resin for desalting or buffer exchange applications. This resin is ideal for removing low molecular weight compounds including salts, fluorescent dyes, biotin, and other small labeling reagents. Protein samples can be processed with unsurpassed protein recovery and 95% retention of salts and other small molecular contaminants (<1,000 Da). Zeba Desalting cartridges provide fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses.

Rapidly desalt protein and biological samples for manual or automated liquid chromatography (LC) with Thermo Scientific Zeba Spin Desalting columns, plates, and cartridges, which enable =95% retention of salts and other small molecular contaminants (<1,000 Da). Zeba Spin Desalting products contain a proprietary high-performance size-exclusion chromatography resin that enables excellent protein desalting and recovery of 7K molecular weight cutoff (MWCO) molecules using a centrifuge format.

nZeba Spin Desalting columns, plates, and chromatography cartridge fittings are convenient, ready-to-use devices compatible with major automated liquid-chromatography systems or for manual syringe processing. The polypropylene columns have a 7K MWCO and contain a proprietary high-performance size-exclusion chromatography resin (Zeba High-Performance Resin) that offers excellent protein desalting and recovery in a centrifuge format. When using Zeba Spin Desalting Columns, even very dilute (25 µg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules (<1000 MW), and good recovery of proteins and other macromolecules (>7000 MW).

Similarly, Zeba Desalting plates (7K MWCO) are polypropylene devices that contain Zeba High-Performance Resin and provide excellent protein desalting performance, with high recovery of proteins >7000 Da in a centrifuge format for small sample volumes. The plates enable fast, consistent, well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations. Zeba 96-well spin desalting plates require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100 µL) in just five minutes, offering rapid and exceptional protein desalting.

The Zeba Desalting Chromatography Cartridges contain Zeba High-Performance Resin for desalting or buffer exchange applications. This resin is ideal for removing low molecular weight compounds including salts, fluorescent dyes, biotin, and other small labeling reagents. Protein samples can be processed with unsurpassed protein recovery and 95% retention of salts and other small molecular contaminants (<1,000 Da). Zeba Desalting cartridges provide fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses.

Benefits of Zeba Spin Desalting columns, plates, and cartridges include:
• High recovery—low-binding resin maximizes protein recovery
• Fast—no fraction screening or waiting for protein to emerge by gravity flow
• Economical—great performance at lower cost than other commercially available cartridges
• Easy-to-use—no cumbersome column preparation or equilibration
• Flexible—available in spin columns, filter spin plates and cartridges for a range of needs

Available formats
• Spin columns—75 µL, 0.5 mL (microcentrifuge), 2 mL, 5 mL, 10 mL columns
• 96-well spin plates—pre-dispensed 96-well filter plates, compatible with centrifugation for manual or automated purification
• Chromatography cartridges—compatible with automated liquid chromatography systems and with syringe processing