1 mL

dTTP (100 mM)

FastAb Adjuvant is a novel, water-soluble mouse adjuvant designed specifically for preparing monoclonal and polyclonal antibodies. It can be used as an alternative to the classic Freund’s adjuvant

Fluorescein-5-isothiocyanate (FITC “Isomer I”) – comjugated rabbit IgG fraction to ß-galactosidase (no cross to E. coli) and buffer salts.

• Routinely used for attachment and maintenance of human embryonic stem cells (hESC)
• Human ESCs have specific needs for attachment and maintenance and some lots of basement membrane extract may not provide adequate support
• Geltrex™ hESC-qualified reduced growth factor basement membrane matrix has been tested for support of hESC growth and pluripotency, therefore minimizing the need to test multiple lots
• Consistent manufacturing processes help ensure a protein concentration averaging 15 mg/mL with very little variation from lot-to-lot

Glutaraldehyde Solution

Alkaline phosphatase (AKP)-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) and buffer salts.

Product is guinea pig serum, rapidly processed to preserve complement activity.

IgG/IgM Depleted Human Serum Heat-Inactivated

L-PROLINOL

Protein Loading Buffer for Native-PAGE- 5X – 1 mL

Protein Loading Buffer for SDS-PAGE- 5X – 1 mL

Ready-to-use proteinase K solution, 20 mg/mL in 10 mM Tris-HCl, 1 mM calcium acetate, pH 7.5 containing 50% glycerol.

Lyophilized rabbit antiserum to bovine thyroxine-BSA

Product is rabbit serum, rapidly processed to preserve complement activity. Pooled rabbit serum is filtered through a 0.22 µm filter, vialed and lyophilized.

RNA Sample Loading Buffer

RNA Sample Loading Buffer

RNA Sample Loading Buffer, No Ethidium Bromide

10x concentrate solution of bromophenol blue, xylene cyanol FF and glycerol for loading samples in the wells of agarose or polyacrylamide gels.

Mouse serum is used for blocking agents and controls in immunoassays.

Ready-to-use PCR mastermix

Accelerate throughput for automated magnetic purification of biotinylated molecules with Thermo Scientific™ Pierce Streptavidin Magnetic Beads.

Magnetic streptavidin beads enable affinity purification of biotin-labeled target molecules without columns or centrifugation. The platform provides a fast and convenient method for manual or automated immunoprecipitation, protein interaction analysis, DNA-protein pull-down, and purification involving biotin-labeled proteins and nucleic acids. The iron oxide superparamagnetic particles offer superior performance (high capacity and low nonspecific binding) compared with other commercial magnetic beads. Biotinylated molecules are simply added to the streptavidin-coated magnetic beads for binding. Pierce Streptavidin Magnetic Beads are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher™ 96 and KingFisher Flex Instruments, but the beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand.

Highlights:

• High-performance beads – non-aggregating, pre-blocked, iron oxide, superparamagnetic microparticles provide exceptional uniformity for automated HTS and manual applications alike
• Stable immobilization chemistry – streptavidin is immobilized using leach-resistant chemistry
• High capacity – superior quality beads with high binding capacity provide rapid and efficient biomolecule purification from complex samples
• Low non-specific binding – stable, pre-blocked beads provide clean purification products (e.g., antigen eluted in IP with biotinylated antibody) that are compatible with mass spectrometry analysis and other downstream analyses
• Superior performance – nearly three times higher binding capacity than typical beads from other suppliers, allowing the use of smaller amounts per experiment

Properties of Streptavidin Magnetic Beads:

• Composition: ion oxide particles covalently coated with a monolayer of streptavidin protein
• Magnetization: superparamagnetic (no magnetic memory)
• Bead size: 1μm (nominal mean diameter)
• Density: 2g/cm³
• Concentration: 10mg beads per milliliter of slurry in 0.05% sodium azide
• Binding capacity: approx. 55μg biotinylated rabbit IgG per mg of beads; approx. 3500pmol biotinylated fluorescein per milliliter of beads
• RNase: these bead solutions are NOT tested and certified to be RNAse-free

Requires:

Thermo Scientific KingFisher 96 or KingFisher Flex Instrument or other magnetic separator device

Recommended for:

Immunoprecipitate antigens (using biotinylated antibodies) from a wide variety of sources; Co-immunoprecipitate interaction complexes using biotinylated antibodies; Capture protein-protein interactions in pull-down assays using biotinylated bait proteins; Isolate biotin-labeled DNA-protein complexes from cell or tissue extracts; Capture single-stranded biotinylated DNA oligos; Isolate biotinylated PCR products

Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. Human β-glucuronidase is a type of glucuronidase that catalyzes hydrolysis of β-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides such as heparan sulfate. In human gut β-glucuronidase converts conjugated bilirubin to the unconjugated form for reabsorption.