10 mL

Brain Pituitary Extract (BPE) is made from fresh, unfrozen pituitary glands from young, healthy cattle less than 30 months old, born, raised and slaughtered in New Zealand from a USDA approved source.

Catalase

Cryopres Dimethyl Sulfoxide is commonly used in cell cryopreservation, along with glycerol, to prevent damage to the cell membrane during freezing.

Ethidium Bromide Solution

A 10 mg/mL ready-to-use solution of EtBr in specially filtered, deionized water.

Ethidium Bromide Solution, Ultra Pure

Freund’s incomplete adjuvant is used for the boosts. Prepared from non-metabolizable oils (paraffin oil and mannide monooleate)

Gentamicin is an aminoglycoside antibiotic complex that inhibits the growth of a wide variety of Gram-positive and Gram-negative microorganisms, including strains resistant to tetracycline, chloramphenicol, kanamycin, and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus, and Streptococcus. It is effective against mycoplasma as well.

Gentamicin is an aminoglycoside antibiotic complex that inhibits the growth of a wide variety of Gram-positive and Gram-negative microorganisms, including strains resistant to tetracycline, chloramphenicol, kanamycin, and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus, and Streptococcus. It is effective against mycoplasma as well.

• • Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G solution is now routinely used with other basal media to support a wide variety of cell types in the presence of 2 to 4% FBS

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• • Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids

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• • Transferrin is an iron carrier and it may also help to reduce toxic levels of oxygen radicals and peroxide

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• • Selenium, as sodium selenite, is a cofactor for glutathione peroxidase and other proteins, and is used as an antioxidant in media

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The most cited neuronal cell culture supplement, B-27 Supplement is an optimized serum-free supplement used to support the low- or high-density growth and short- or long-term viability of embryonic, post-natal, and adult hippocampal and other CNS neurons. B-27 Supplement is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.

• • Provided as 50X liquid

• • Used with Neurobasal™ Medium for neuronal cell culture

• • Tested in growth assay utilizing rat (Sprague Dawley) embryonic hippocampal neurons

• • Tested also for endotoxin at a 1X concentration

Glutaraldehyde Solution

Glutaraldehyde Solution

Description

Thermo Scientific™ Halt Protease Inhibitor Cocktails are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction procedures.

nHalt Protease Inhibitor Cocktails are 100X solutions containing optimized concentrations of six broad-spectrum protease inhibitors stabilized in high-quality dimethylsulfoxide (DMSO). The cocktails effectively inhibit serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. The identity and concentration of each component is fully disclosed, leaving no uncertainties about variables and compatibility with experiments. Regular and EDTA-free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis, respectively. The stable, concentrated liquid cocktails are easy to use, and the several different package sizes make Halt Protease Inhibitor Cocktails exceptionally convenient for either small-scale or large-scale uses. Highlights:

• • Better than tablets – the refrigerator-stable, 100X liquid format is more effective and easier to use than individual inhibitors or other formats; just pipette exactly the amount you need

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• • No proprietary ingredients – fully disclosed formulation contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A in DMSO

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• • Multiple package sizes – choose among several package sizes, including single-use microtubes that keep protease inhibitors fresh and reduce the risk of reagent contamination

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• • Two popular formulations – regular formulation includes a separate vial of EDTA solution for optional metalloprotease inhibition; EDTA-free formulation ensures compatibility with isoelectric focusing

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• • Lysis buffer compatible – use with Thermo Scientific Pierce™ Cell Lysis Buffers or nearly any other commercial or homemade detergent-based lysis reagent

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nIncludes:nnProtease inhibitor cocktails with EDTA include separate 0.5M EDTA solution.

Heptafluorobutyric Acid

IgG/IgM Depleted Human Complement

IgG/IgM Depleted Human Serum Heat-Inactivated

Mouse Serum(C57BL/6 background) is a pooled preparation obtained from a normal mouse population. Blood is collected from good quality, normal, healthy mice. The serum, pooled and sterile filtered, shows minimal
hemolysis.

Rat serum is pooled from a normal donor population.

NuPAGE Sample Reducing Agent contains 500mM dithiothreitol (DTT) at a ready-to-use, 10X concentration in a stabilized liquid form. Use NuPAGE Sample Reducing Agent when running proteins on NuPAGE Novex Bis-Tris gels to maintain protein samples in a reduced state during electrophoresis.nn1D Gel Electrophoresis, 2D Gel Electrophoresis, IsoElectric Focusing, Protein Gel Electrophoresis, Protein Sample Fractionation, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis

Orange G/blue loading dye (6X)

Orange G/blue tracking dye (6X)

Phosphatase Inhibitor Cocktail – 10X – 10mL

Protease and Phosphatase Inhibitor Cocktail – 10X – 10 mL

Ready-to-use proteinase K solution, 20 mg/mL in 10 mM Tris-HCl, 1 mM calcium acetate, pH 7.5 containing 50% glycerol.

Proteinase K, Ready-to-use

Pyronin Y, Solution (10 mg/ml), Electrophoresis Grade

SOC (sterile solution)

Squalene