10 mL

TBE Urea Sample Buffer (2X)

Catalyst for polyacrylamide gel polymerization

Perform ribonucleotide and oligonucleotide RNA isolation with Thermo Scientific™ Pierce Boronic Acid Resin.

This immobilized boronic acid is an easy-to-use polyacrylamide affinity support for purification of ribonucleotides and other small molecular weight compounds that contain cis-diol groups. The ligand (m-aminophenyl-boronic acid) binds to the cis-diol groups on the sugar portion of nucleotides, forming a reversible five-member ring complex. After washing away non-bound molecules from the sample, the complex can be dissociated and the nucleotide eluted by lowering the pH or by addition of sorbitol.

Highlights:

• Ligand – boronic acid
• Support – beaded polyacrylamide (1800 MW exclusion limit)
• Spacer – m-aminophenyl group
• Loading – 100μmol boronate per milliliter of settled resin
• Capacity – at least 99% binding and recovery of 110μmol AMP per milliliter of settled resin
• Format – 50% resin slurry in 0.05% sodium azide

Rapidly desalt protein and biological samples for manual or automated liquid chromatography (LC) with Thermo Scientific Zeba Spin Desalting columns, plates, and cartridges, which enable =95% retention of salts and other small molecular contaminants (<1,000 Da). Zeba Spin Desalting products contain a proprietary high-performance size-exclusion chromatography resin that enables excellent protein desalting and recovery of 7K molecular weight cutoff (MWCO) molecules using a centrifuge format.

nZeba Spin Desalting columns, plates, and chromatography cartridge fittings are convenient, ready-to-use devices compatible with major automated liquid-chromatography systems or for manual syringe processing. The polypropylene columns have a 7K MWCO and contain a proprietary high-performance size-exclusion chromatography resin (Zeba High-Performance Resin) that offers excellent protein desalting and recovery in a centrifuge format. When using Zeba Spin Desalting Columns, even very dilute (25 µg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules (<1000 MW), and good recovery of proteins and other macromolecules (>7000 MW).

Similarly, Zeba Desalting plates (7K MWCO) are polypropylene devices that contain Zeba High-Performance Resin and provide excellent protein desalting performance, with high recovery of proteins >7000 Da in a centrifuge format for small sample volumes. The plates enable fast, consistent, well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations. Zeba 96-well spin desalting plates require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100 µL) in just five minutes, offering rapid and exceptional protein desalting.

The Zeba Desalting Chromatography Cartridges contain Zeba High-Performance Resin for desalting or buffer exchange applications. This resin is ideal for removing low molecular weight compounds including salts, fluorescent dyes, biotin, and other small labeling reagents. Protein samples can be processed with unsurpassed protein recovery and 95% retention of salts and other small molecular contaminants (<1,000 Da). Zeba Desalting cartridges provide fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses.

From Helix pomatia . Sulfatase Activity ≤5000 u/ml