10000U

Deoxyribonuclease
I (DNase I) is DNA-specific endonuclease that cleaves both single-stranded DNA,double-stranded
DNA and DNA-RNA hybrids, yielding 5′-phosphate-terminated polynucleotides with
a free hydroxyl group on position 3′ producing tetranucleotides. The activity of DNase I is strictly
dependent on Ca²⁺ and can be activated by divalent metal ions such
as Mg²⁺ or Mn²⁺. In the presence of Mg²⁺,
DNase I nonspecifically recognizes and cleaves a double-stranded DNA at anysite
on either strand, and in the presence of Mn²⁺, it recognizes and
cleaves almost the same sites on both strands of the DNA to produce DNA
fragments with blunt ends or sticky ends with 1~2 nucleotide overhangs.

GenScript is
offering DNase I produced by expression in a P. pastoris strain carrying a plasmid encoding the bovine DNase I.

GenScript M-MuLV Reverse Transcriptase (M-MLV) is derived from a cloned region of the pol gene of MMLV and isolated from an E. coli strain overexpressing this construct. To increase cDNA yields and get a higher percentage of longer transcripts, the M-MLV Reverse Transcriptase has been modified with reduced RNase H activity, and expressed free of exogenous RNases and other nucleases. The enzyme can synthesize a complementary cDNA strand initiating from a primer using RNA as template (cDNA synthesis), making it ideal for a wide range of applications.