1000U

Deoxyribonuclease
I (DNase I) is DNA-specific endonuclease that cleaves both single-stranded DNA,double-stranded
DNA and DNA-RNA hybrids, yielding 5′-phosphate-terminated polynucleotides with
a free hydroxyl group on position 3′ producing tetranucleotides. The activity of DNase I is strictly
dependent on Ca²⁺ and can be activated by divalent metal ions such
as Mg²⁺ or Mn²⁺. In the presence of Mg²⁺,
DNase I nonspecifically recognizes and cleaves a double-stranded DNA at anysite
on either strand, and in the presence of Mn²⁺, it recognizes and
cleaves almost the same sites on both strands of the DNA to produce DNA
fragments with blunt ends or sticky ends with 1~2 nucleotide overhangs.

GenScript is
offering DNase I produced by expression in a P. pastoris strain carrying a plasmid encoding the bovine DNase I.

Hot Start Taq DNA Polymerase is a recombinant, thermostable Taq DNA polymerase complexed with a thermolabile, neutralizing antibody that blocks the polymerase activity prior to the initial DNA denaturation step of PCR. When the temperature of the PCR reaction mix reaches 95°C during the initial DNA denaturing step of PCR cycling, activity of the Taq DNA polymerase is fully restored.