100RXN

ABQubit 1X dsDNA HS Assay Kit (Pre-Mixed)

ABQubit dsDNA HS Assay Kit

ABQubit ssDNA Assay Kit

ABscript II Reverse transcriptase is a recombinant M-MuLV reverse transcriptase with low RNase H activity and enhanced thermal stability.Compared to wild-type M-MuLV,recombinant M-MuLV reverse transcriptase can synthesize first-strand cDNA at higher temperatures.The enzyme is active at up to 48℃ with higher specificity and cDNA yield.The kit provides two reverse transcription primers.The Oligo-dT primer [d(T)₂₃VN] forces the primer to anneal to the beginning of the polyA tail.The optimized random primer premix has low specificity,and all RNA,including mRNA,rRNA,and tRNA can be used as a reverse transcription template.This kit can amplify cDNA up to 10 kb.

ABScript III RT Master Mix for qPCR is developed based on ABScript II Reverse Transcriptase and suitable for two-step RT-qPCR detection. 5X ABScript III RT Mix contains all the reagents needed for reverse transcription reaction (ABScript III Reverse Transcriptase, RNase Inhibitor, RandomPrimer, Oligo dT Primer, dNTP and reaction buffer), and a reaction can be started simply by adding template RNA and Nuclease-free H₂O.
This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)₂₀VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance gene expression analysis.

ABScript III RT Master Mix for qPCR with gDNA Remover is developed based on ABScript III Reverse Transcriptase and suitable for two-step RT-qPCR detection. The 5X ABScript III RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H2O. The gDNA Remover Mix in this product can completely remove the genomic DNA remaining in the RNA template and make the qPCR results more accurate. The dsDNase is heat-sensitive and can be quickly and irreversibly inactivated under high temperature conditions. Therefore, it only needs one sample to be used to remove genomic DNA contamination and reverse transcription reactions in the same tube.
This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)₂₀VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance geneexpression analysis.

ABScript Neo RT Master Mix for qPCR is an efficient and fast cDNA first-strand synthesis master mix, suitable for two-step RT-qPCR detection. The 4X ABScript Neo RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H₂O.
This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)20VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance gene expression analysis.

ABScript Neo RT Master Mix for qPCR with gDNA Remover is an efficient and fast cDNA first-strand synthesis master mix, suitable for two-step RT-qPCR detection. The 4X ABScript Neo RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H₂O. The gDNA Remover Mix in this product can completely remove the genomic DNA remaining in the RNA template and make the qPCR results more accurate. The dsDNase is heat-sensitive and can be quickly and irreversibly inactivated under high temperature conditions. Therefore, it only needs one sample to be used to remove genomic DNA contamination and reverse transcription reactions in the same tube.

This kit is a nucleic acid extraction kit based on magnetic beads, primarily designed for isolating and purifying high-quality plasmid DNA from bacteria. It utilizes the functional groups on the surface of magnetic beads to enrich nucleic acids from sample lysates onto the surface of the magnetic beads, followed by separation of the magnetic beads using a magnetic separation device to achieve rapid isolation and purification of nucleic acids. This reagent kit boasts high efficiency, speed, and convenience, and when paired with a fully automatic nucleic acid extraction instrument, it can complete the extraction of 96 samples in just approximately 25 minutes.

This kit is a nucleic acid extraction kit based on magnetic beads. The main principle involves using functional groups on the surface of the magnetic beads to enrich nucleic acids from the sample lysate onto the surface of the beads. The beads are then washed to remove proteins and other impurities. Then, a magnetic separation device is used to isolate the beads, allowing for rapid separation and purification of nucleic acids. The entire process does not require the use of organic reagents such as phenol, chloroform, or β-mercaptoethanol, making it safe and non-toxic. The use of paramagnetic magnetic beads is suitable for high-throughput automated extraction. The extracted product can be directly used for RT-PCR, RT-qPCR, Northern Blot, next-generation sequencing library construction, and cloning.

This kit uses magnetic bead-based technology for nucleic acid purification. It utilizes functional groups on magnetic beads to enrich nucleic acids from sample lysates onto the bead surface.The beads are then washed to remove proteins and other impurities. After that, a magnetic separation device is used to separate the magnetic beads, and then DNase I is used for digestion to remove DNA, so as to rapidly separate and purify RNA. The whole process does not require the use of phenol, chloroform and β-merphytoethanol and other harmful substances, safe and non-toxic, and the use of paramagnetic magnetic beads, suitable for high-throughput automated extraction.

AFTMag Quick FFPE DNA Extraction Kit

This kit uses magnetic bead-based technology for nucleic acid purification. It utilizes functional groups on magnetic beads to enrich nucleic acids from agarose gel onto the bead surface.The beads are then washed to remove impurities, and then separate magnetic beads with magnetic separation device, so as to quickly separate and purify DNA from samples. The whole process does not require the use of phenol, chloroform and β-merphytoethanol,, safe and non-toxic, and the use of paramagnetic magnetic beads, suitable for high-throughput automated extraction.

This kit is a nucleic acid extraction kit based on magnetic beads. The main principle involves using functional groups on the surface of the magnetic beads to enrich nucleic acids from the sample lysate onto the surface of the beads. The beads are then washed to remove proteins and other impurities. Then, a magnetic separation device is used to isolate the beads, allowing for rapid separation and purification of nucleic acids. The entire process does not require the use of organic reagents such as phenol, chloroform, or β-mercaptoethanol, making it safe and non-toxic. The use of paramagnetic magnetic beads is suitable for high-throughput automated extraction.

This kit is a nucleic acid extraction kit based on magnetic beads with high-specificity binding. The main principle involves utilizing the functional groups on the surface of the magnetic beads to enrich nucleic acids from the sample lysate onto the surface of the beads. The proteins and other impurities are removed with washing buffer, followed by separation of the beads using a magnetic separation device. DNase I digestion is then employed to remove DNA, allowing for the rapid separation and purification of RNA. The entire process does not require the use of harmful substances such as phenol, chloroform, or β-mercaptoethanol, making it safe and non-toxic. Moreover, the paramagnetic magnetic beads are suitable for high-throughput automated extraction. The extracted products can be directly used in downstream experiments such as RT-PCR, qRT-PCR, Northern Blot, next-generation sequencing library preparation, and molecular cloning.

This kit is a nucleic acid extraction kit based on magnetic bead separation and purification. Its main principle is to use the functional groups on the surface of functional bio – magnetic beads to enrich nucleic acids from the sample lysate onto the surface of the magnetic beads. Then, a magnetic separation device is used to separate the magnetic beads, thus enabling the rapid separation and purification of nucleic acids. During the extraction process, there is no need to use toxic phenol – chloroform extraction, nor time – consuming alcohol precipitation. This kit can be integrated with a magnetic rod method or pipetting method automatic nucleic acid extractor for high – throughput extraction experiments. It can also be manually operated using a magnetic separation rack.

This kit is a nucleic acid extraction kit based on magnetic beads. The main principle involves using functional groups on the surface of the magnetic beads to enrich nucleic acids from the sample lysate onto the surface of the beads. The beads are then washed to remove proteins and other impurities. Then, a magnetic separation device is used to isolate the beads, allowing for rapid separation and purification of nucleic acids. The entire process does not require the use of organic reagents such as phenol, chloroform, or β-mercaptoethanol, making it safe and non-toxic. The use of paramagnetic magnetic beads is suitable for high-throughput automated extraction. The extracted product can be directly used for RT-PCR, RT-qPCR, Northern Blot, next-generation sequencing library construction, and cloning.

Entrans 2X qPCR Probe Master Mix is a special reagent for qPCR reactions based on the primer-probe detection. When a probe is bound to the template DNA strand, the 5′-3 ‘exonuclease activity of Taq enzyme can be used to hydrolyze the probe, thereby releasing fluorescent signal. This product uses chemically modified hot-start Taq enzyme for amplification. Accurate quantification, high amplification efficiency, good repeatability and wide credibility. Entrans 2X qPCR Probe Master Mix is a 2X concentration master mix, which contains all the components required for qPCR except primers, probes and templates.

Entrans 2X SNP Genotyping qPCR Probe Master Mix (with UDG)

Entrans qPCR Probe Kit is designed for probe-based qPCR reactions. It contains all components required for a rapid and reliable qPCR reaction except primers, probes and templates. When an intact probe binds to the complementary template DNA strand, the reporter dye and quencher are in proximity and the quencher absorbs the fluorescence emitted by the reporter dye. In PCR extension, the 5 ‘-3 ‘exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. This kit includes an engineered version of Taq polymerase with antibody-mediated Hot-start capability to inhibit non-specific amplification at room temperature, while allowing high efficiency, sensitivity and reproducibility in qPCR amplification. The Entrans qPCR Probe Kit is an ideal product for high-speed multiplex qPCR analyses.

This product utilizes HS Taq DNA Polymerase as the core enzyme and adopts a dual modification method with chemical antibody and modification, aiming to ensure amplification efficiency while significantly enhancing the specificity of the product, suitable for single nucleotide polymorphism (SNP) genotyping detection.

The Fast One Step Probe RT-qPCR Kit is a reagent kit designed for rapid amplification quantitative RT-qPCR detection using the probe method. It includes reaction buffer, hot-start Taq polymerase, novel reverse transcriptase ABScript Ⅲ Reverse Transcriptase, and heat-labile UDG. This kit utilizes RNA as a template and includes gene-specific primers, enabling consecutive reverse transcription and PCR reactions to take place in the same tube without the requirement for additional pipetting or tube opening steps. This streamlined process significantly enhances throughput. The kit utilizes dUTP/UDG to prevent contamination, with the heat-sensitive UDG rapidly degrading uracil-containing contaminants at room temperature. At 50°C during reverse transcription, the heat-labile UDG quickly deactivates, ensuring it does not affect the efficiency and sensitivity of RT-qPCR.

The Lyophilization One-Step RT-qPCR Probe Kit is a universal lyophilized reagent designed for RT-qPCR reactions using probes. It is suitable for lyophilization of freeze-dried powder and freeze-dried microspheres. After freeze-drying into microspheres. This kit includes reaction buffer, hot-start Taq polymerase, novel reverse transcriptase ABScript Ⅲ Reverse Transcriptase, heat-labile UDG, and RNase inhibitor (RI). This kit utilizes RNA as a template and employs gene-specific primers. Reverse transcription and PCR reactions can be performed consecutively in the same tube, eliminating the need for additional tube opening or pipetting steps, significantly enhancing detection throughput. The kit utilizes dUTP/UDG for contamination prevention, with the heat-labile UDG quickly degrading U-containing contaminants at room temperature. The heat-labile UDG is rapidly deactivated at 50°C during reverse transcription, ensuring it does not affect the efficiency and sensitivity of RT-qPCR. This product contains glycerol content below 0.2% and includes lyoprotectants and excipients. It can be stored at 45°C for 6 months without degradation in performance.

Mouse Tissue Direct PCR Kit is specially designed for the rapid genotyping of mouse, which contains Tissue Lysis Buffer II and Powerpol HS 2X PCR Mix for Mouse Genotyping for DNA release and PCR amplification. This kit can be used for the rapid extraction of genomic DNA from mouse tails, ears, toes, and other tissues. The extracted genomic DNA can be used directly as template for PCR amplification, which greatly shortens the experimental time. Tissue Lysis Buffer II in this kit already contains proteinase K, which does not require additional preparation.
Powerpol HS 2X PCR Mix for Mouse Genotyping in this kit only needs to add primers and templates to perform amplification, and the reagent contains loading buffer, PCR products can be directly loaded for electrophoresis.