1mg

The GenCRISPR™
Ultra eSpCas9 product line provides customers with a selection of research use,
basic GMP and GMP compliant Cas9 nucleases. The Cas9 protein can be formed with
the guide RNA into a ribonucleoprotien (RNP) complex. The use of an RNP complex
to perform gene editing has been shown to reduce the challenges encountered
with other CRISPR gene editing techniques such as viral and plasmid delivery.
Challenges include off-target effects, cell viability and transcription/translational
challenges.

GenCRISPR™ Ultra eSpCas9-N-NLS
Research, tag-free is a mutant form of Cas9
nuclease and is produced by expression in an E. coli strain carrying a plasmid encoding the eSpCas9 gene from Streptococcus pyogenes with an N-terminal
nuclear localization signal (N-NLS). GenCRISPR™ Ultra eSpCas9-N-NLS Research,
tag-free delivers higher fidelity
and less off-target activity than wild-type SpCas9 nuclease.

GenCRISPR™ Ultra NLS-Cas9-basic GMP is utilized for CRISPR gene editing applications. The Cas9 nuclease forms a stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component. With the help of nuclear localization signal (NLS) expressed with the Cas9 nuclease, the RNP complex enters the nucleus and cleaves the target gene. When compared with a plasmid-based delivery system, the RNP delivery system has been observed to increase the on-target gene editing efficiency and decrease off-target effects.

The
GenCRISPR™ Ultra Cas9 product line provides customers with a selection of
research use, GMP preclinical and GMP compliant Cas9 nucleases. The Cas9
protein can be formed with the guide RNA into a ribonucleoprotien (RNP)
complex. The use of an RNP complex to perform gene editing has been shown to
reduce the challenges encountered with other CRISPR gene editing techniques
such as viral and plasmid delivery. Challenges include off-target effects, cell
viability and trascprition/translational challenges.

GenCRISPR™ Ultra Cas9 is
produced by expression in an E. coli
strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes with an N-terminal nuclear localization
signal (NLS).

Molecular Formula : C29 D3 H28 N7 O

Molecular Formula : C21H16Na2O14S

Molecular Formula : C15 2H4 H6 O5

Molecular Formula : C21H43N5O7.5C2H4O2

Molecular Formula : C29H59N5O17

Molecular Formula : C20 H41 N5 O7 . 5 C2 H4 O2

Molecular Formula : C18 H32 O16

Molecular Formula : C20H29D5O

Molecular Formula : C15H24

Molecular Formula : C21 H25 O5 S . C5 H6 N

Molecular Formula : C21 D7 H19 O2

Molecular Formula : C25 H24 N4 O2

Growth Hormone (GH) is a member of the somatotropin/prolactin family which play an important role in growth control. The human GH cDNA encodes a 217 amino acid (aa), and the first 26 aa is a signal peptide. By alternative splicing, at least four isoforms of GH have been identified. The major role of GH in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. GH stimulates both the differentiation and proliferation of myoblasts, and also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

Growth Hormone (GH) is a member of the somatotropin/prolactin family which play an important role in growth control. The human GH cDNA encodes a 217 amino acid (aa), and the first 26 aa is a signal peptide. By alternative splicing, at least four isoforms of GH have been identified. The major role of GH in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. GH stimulates both the differentiation and proliferation of myoblasts, and also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

Growth Hormone (GH), is a member of the somatotropin / prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.

Molecular Formula : C149H249N47O42 . x(CH3COOH)

Molecular Formula : C32H50N10O5 . xC2HF3O2

Molecular Formula : C34H37N7O4 .xC2HF3O2

Molecular Formula : C43H45N7O7 .xC2HF3O2

Molecular Formula : 13C C18 D2 H20 O6

Molecular Formula : C19 H24 O6

Molecular Formula : C19 H24 O5

Molecular Formula : C19H22O6

Molecular Formula : C19 H24 O7

Molecular Formula : C19H24O4

Molecular Formula : C48 H82 O18

Molecular Formula : C36H60O8

Molecular Formula : C41 H64 O14

GITR Ligand, also known as TNFSF18 and TL6, is an approximately 30 kDa type II transmembrane glycoprotein in the TNF superfamily (1). Human GITR Ligand consists of a 50 amino acid cytoplasmic domain, a 21 aa transsmembrane segment, and a 128 aa extracellular domain (ECD). Within the ECD, human GITR Ligand shares 56% and 60% aa sequence identity with mouse and rat GITR Ligand, respectively. TNFSF18 is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. GITRL/TNFSF18 is up-regulated after stimulation by bacterial lipopolysaccharides (LPS). TNFSF18 Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. TNFSF18 / GITR Ligand is important for interactions between activated T-lymphocytes and endothelial cells.Recombinant Human GITR Ligand Fc Chimera produced in HEK293 cells is a polypeptide chain containing 359 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhGITRL has a molecular mass of 50 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Molecular Formula : C15 2H4 H17 N3 O3 S

Molecular Formula : C24H29D5N4O5S

Molecular Formula : C13 H14 N2 O4 S2

Molecular Formula : C21 D11 H16 N5 O4 S

Molecular Formula : C19H14F3N3O3

Molecular Formula : C153H225N43O49S . x(C2HF3O2)

Molecular Formula : C153H216D9N43O49S x(C2HF3O2)

Molecular Formula : C12 H23 N O10 S3

Molecular Formula : C24H47NO7

Molecular Formula : C5 H12 N O4 P

Molecular Formula : C5 2H3 H9 N O4 P . Cl H

Molecular Formula : C813C2H17N215NO6S • C2HF3O2

Molecular Formula : C10H12D5N3O6S (free acid)

Glutathione S-Transferase (GST), an antioxidant enzyme, is involved in the primary cellular defense mechanism against reactive oxygen species. GST is soluble in water and has a mass of 26.98 kDa. It occurs as a dimer in all aerobic organisms.

Molecular Formula : C23 D11 H17 Cl N3 O5 S

Molecular Formula : C5 H13 N Na O6 P

Glycerolkinase

Molecular Formula : C25H43D5O3