250U
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Hot Start Taq DNA Polymerase
Hot Start Taq DNA Polymerase is a recombinant, thermostable Taq DNA polymerase complexed with a thermolabile, neutralizing antibody that blocks the polymerase activity prior to the initial DNA denaturation step of PCR. When the temperature of the PCR reaction mix reaches 95°C during the initial DNA denaturing step of PCR cycling, activity of the Taq DNA polymerase is fully restored.
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SUMO Protease Animal-Free, His
SUMO protease, also known as Ulp, is a protease
that specifically removes small ubiquitin-related modifier (SUMO) in any recombinant SUMO fusion protein. Different
from other tag removal proteases such as enterokinase (EK) and TEV which
recognize a specific amino acid sequence and cut at a specific cleavage site,
SUMO protease recognizes the SUMO tertiary structure and cleave it, more
specifically and leaving no residual amino acids.
GenScript’s SUMO Protease Animal-Free, His, is
the recombinant SUMO protease fragment from Saccharomyces
cerevisiae. This recombinant enzyme is expressed in E.coli and purified to obtain high yields of the active enzyme. It
is produced under an animal free process, and is suitable for drug and vaccine
development, manufacture and other applications. This product is designed with
a C-terminal 6x His tag which can be removed after the SUMO cleavage reaction
by purification using Ni2+ affinity chromatography resin (Cat. No. L00223) or Ni-charged magnetic beads (Cat. No. L00295).