2ml (0.5 ml Settled Beads)

The GenScript Glutathione
MagBeads are developed for quick and efficient small‐scale purification of
recombinant glutathione‐S‐transferase (GST)-tagged fusion proteins from
bacteria, yeast and mammalian crude cell lysates.

GenScript’s Glutathione
MagBeads are superparamagnetic beads pre-coupled with reduced glutathione (GSH).
For purification, samples containing the target proteins are added to the
Glutathione MagBeads. The GST-tagged proteins bind to GSH on the MagBeads. The beads
are then washed and the GST-tagged proteins are eluted. Magnetic separation simplifies protein purification process by eliminating
the need for centrifugation, minimizes sample loss
and removes the excessive steps of a centrifugation-based purification process.

GenScript Protein A/G MagBeads are superparamagnetic beads covalently bound with recombinant proteins A and G for antibody purification. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Protein A/G MagBeads have a binding capacity of more than 10 mg Goat IgG per 1 ml settled beads.
Protein A/G is a genetically engineered protein (MW ≈43 kDa) that combines the IgG binding sites of both Protein A and Protein G. 6×His-tag was attached to its N-terminal to facilitate the purification. The secreted Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a more universal tool to bind and purify immunoglobulins.

Product Details

The
GenScript Protein A/G MagBeads MX are ideal for small‐scale purification of antibodies
from  plasma, serum, ascites, and cell
culture.

The
Protein A/G is a genetically engineered protein (MW≈43 kDa) that combines the
IgG binding sites of both Protein A and Protein G. It contains four Fc‐binding
domains from Protein A and two from Protein G, making it a universal tool to
bind and purify immunoglobulins (IgGs). GenScript’s Protein A/G MagBeads MX have
Protein A/G pre-coupled on the surface of superparamagnetic beads. To purify
antibodies, a sample containing the antibody of interest is incubated with the Protein
A/G MagBeads MX. The antibody binds to the Protein A/G on the beads during a
short incubation. This is followed by washing the beads to remove
non-specifically bound materials, and eluting the purified antibody. Magnetic
separation simplify purification process by eliminating the need for
centrifugation, minimizes sample loss and removes the excessive steps of a traditional
centrifugation-based purification method.

GenScript Protein G MagBeads are superparamagnetic beads covalently bound with recombinant protein G for antibody purification. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Protein G MagBeads have a binding capacity of more than 10 mg Goat IgG per 1 ml settled beads.
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native Protein G has three IgG binding domains and also the sites for albumin and cell-surface binding. Albumin and cell-surface binding domains have been eliminated from recombinant Protein G to reduce nonspecific binding. Protein G has greater affinity than Protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike Protein A, Protein G does not bind to human IgM, IgD and IgA.

Product Details

Intended Use
GenScript Streptavidin
MagBeads are ideal for fast and convenient purification of biotinylated
proteins, peptides, antibodies and oligonucleotides, and protein interaction
studies.

Principle
Add the sample containing
biotinylated molecules to the Streptavidin MagBeads and allow the molecules
bind to the MagBeads during a short incubation. Then separate the molecule‐bound beads with a magnetic separation rack. With indirect capture, mix
the biotinylated molecule with the sample to capture the molecule‐target complex before adding the MagBeads.

Description of Material
GenScript Streptavidin MagBeads
are super paramagnetic beads of approximately 45-100
μm in diameter, covalently coated with highly
purified streptavidin. These magnetic beads are composed of magnetite core
encapsulated by agarose. The magnetic beads have streptavidin as its surface
protein which is 53kDa. in size having four binding site per molecule. These
beads have pH stability of 2 to 14 (short term); 3 to 13 (long term). The beads
are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4,
containing 20% ethanol.

Storage and Stability
This product is stable
until the expiration date stated on the COA, when stored unopened at 2–8°C. Do NOT freeze the product. Keep
the MagBeads in liquid suspension during storage and all handling steps. Drying
will cause loss of binding capacity and result in reduced performance.
Resuspend the beads well before use. Be careful to avoid bacterial/fungal
contamination.