5 mL

CAS Number: 462-94-2

The anti-globulin or Coombs’ test is widely used to detect the presence of antibody in the diagnosis of autoimmune hemolytic anemia.

Diethyl Pyrocarbonate

Lyophilized goat antibody to mouse IgA (alpha chain)

Pooled guinea pig serum is filtered through a 0.22 µm filter. Rapidly processed to preserve complement activity.

Lyophilized goat IgG fraction to chicken egg albumin and buffer salts.

Lyophilized goat IgG fraction to human albumin and buffer salts.

Polyclonal Antibody against human IgA alpha chain. Suitable for immunostaining and immunodetection.

Lyophilized goat IgG fraction to human IgM (5Fc μ) and buffer salts.

Lyophilized goat IgG fraction to mouse complement C3 and buffer salts.

Lyophilized rabbit polyclonal antiboody againts human red blood cells. For immunoblotting, immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.

ITS Premix stimulates cell proliferation while decreasing substantially the serum requirements for culture of many cell types. Contains 0.5 mg/ml insulin from bovine pancreas, 0.5 mg/ml human transferrin (substantially iron-free) and 0.5 µg/ml sodium selenite. Prepared in Earle’s balanced Salt Solution (EBSS) without phenol red. Sterile-filtered.

L-PROLINOL

Mineral Oil

Eliminates mycoplasma contamination from cell cultures. Broad spectrum activity. Effective at low concentration. Proven low in cytotoxicity

N-Methylphenethylamine

Nonidet P-40 (substitute)

Pristane

Proteinase K, Ready-to-use

The complement is produced from a large pool of New Zealand White rabbits, 8-12 weeks old. The animals are of United States origin. Cytotoxic titer is determined by NIH standards for microlymphocytotoxicity. Minimum release criteria is 80%-100% cell death in 1:2 dilution.

Rat Liver S9 Homogenate is a Supernatant fraction obtained from an organ (usually liver) homogenate by centrifuging at 9000 g for 20 minutes in a suitable medium; this fraction contains cytosol and microsomes. The microsomes component of the S9 fraction contain cytochrome P450 isoforms (phase I metabolism) and other enzyme activities. The cytosolic portion contains the major part of the activities of transferases (phase II metabolism).

Sample loading buffer for agarose gel electrophoresis (bromophenol blue and sucrose).

Sample loading buffer for agarose gel electrophoresis (bromophenol blue, xylene cyanol FF and glycerol).

Mouse serum is used for blocking agents and controls in immunoassays.

Accelerate throughput for automated magnetic purification of biotinylated molecules with Thermo Scientific™ Pierce Streptavidin Magnetic Beads.nn nnMagnetic streptavidin beads enable affinity purification of biotin-labeled target molecules without columns or centrifugation. The platform provides a fast and convenient method for manual or automated immunoprecipitation, protein interaction analysis, DNA-protein pull-down, and purification involving biotin-labeled proteins and nucleic acids. The iron oxide superparamagnetic particles offer superior performance (high capacity and low nonspecific binding) compared with other commercial magnetic beads. Biotinylated molecules are simply added to the streptavidin-coated magnetic beads for binding. Pierce Streptavidin Magnetic Beads are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher™ 96 and KingFisher Flex Instruments, but the beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand.

Thermo Scientific™ Bond-Breaker TCEP Solution, Neutral pH is a stable, 0.5M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulfide bonds.

nHighlights:n

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  • Ready-to-use, odorless, stable and neutral 0.5M TCEP solution

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• • Eliminates TCEP·HCl stock solution preparation and neutralization prior to SDS-PAGE gel loading

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• • Convenient, time-saving format for TCEP reductions

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• • Neutral pH minimizes possibility of amide bond cleavage during reduction

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• • Room temperature-stable, saves valuable refrigerator space

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• • Contributes to more pleasant, safer laboratory environment

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Thermo Scientific™ Pierce Ethidium Bromide is available as both dilute (0.625mg/mL) and more concentrated (10mg/mL) solutions to accommodate most molecular biology applications for fluorescent DNA visualization.

Thermo Scientific™ Pierce Ethidium Bromide is available as both dilute (0.625mg/mL) and more concentrated (10mg/mL) solutions to accommodate most molecular biology applications for fluorescent DNA visualization.

Highlights:

0.625mg/mL EtBr Solution:

• Each drop contains 25μg of ethidium bromide and is ideal for a 50mL agarose gel
• After adding one drop of solution per 50mL of gel, the final concentration of EtBr will be 0.5μg/mL, which is the recommended concentration for electrophoresis of nucleic acids
• Can also be used as the running buffer or for staining gels after electrophoresis

10mg/mL EtBr Solution:

• A safe and convenient way to use ethidium bromide while minimizing exposure

From Helix pomatia . Sulfatase Activity ≤5000 u/ml

γ-Methacryloxypropyl-Trimethoxysilane