5000U
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DNase I
$237.19 Add to cart View Product DetailsDeoxyribonuclease
I (DNase I) is DNA-specific endonuclease that cleaves both single-stranded DNA,double-stranded
DNA and DNA-RNA hybrids, yielding 5′-phosphate-terminated polynucleotides with
a free hydroxyl group on position 3′
dependent on Ca2+ and can be activated by divalent metal ions such
as Mg2+ or Mn2+. In the presence of Mg2+,
DNase I nonspecifically recognizes and cleaves a double-stranded DNA at anysite
on either strand, and in the presence of Mn2+, it recognizes and
cleaves almost the same sites on both strands of the DNA to produce DNA
fragments with blunt ends or sticky ends with 1~2 nucleotide overhangs.GenScript is
offering DNase I produced by expression in a P. pastoris strain carrying a plasmid encoding the bovine DNase I. -
Enterokinase Animal-Free, His, Bovine
$905.63 Add to cart View Product DetailsEnterokinase (EK) is a serine protease produced
by cells of the duodenum, and is involved in mammalian digestion. It plays the role
of turning trypsinogen to the active form of trypsin, and indirectly activates
the pancreatic digestive enzymes. Enterokinase is a specific protease that
cleaves after a lysine at its cleavage site: Asp-Asp-Asp-Asp-Lys. Enterokinase does
not function if the cleavage site is followed by a proline.
GenScript’s Enterokinase Animal-Free, His,
Bovine is the light chain catalytic subunit of bovine enterokinase consisting
of a single glycosylated polypeptide chain containing Ile801-His1035 (Accession
# P98072). This recombinant protein is expressed in P. pastoris and purified to obtain high yields of the active
enzyme. It is produced under an animal free process, and is suitable for drug and
vaccine development, manufacture and other applications. This product is
designed with a C-terminal 6 × His tag and can be removed after cleavage by Ni2+ affinity
chromatography in resin (Cat. No. L00223) or magnetic
beads (Cat. No. L00295). -
SUMO Protease Animal-Free, His
$434.70 Add to cart View Product DetailsSUMO protease, also known as Ulp, is a protease
that specifically removes small ubiquitin-related modifier (SUMO) in any recombinant SUMO fusion protein. Different
from other tag removal proteases such as enterokinase (EK) and TEV which
recognize a specific amino acid sequence and cut at a specific cleavage site,
SUMO protease recognizes the SUMO tertiary structure and cleave it, more
specifically and leaving no residual amino acids.
GenScript’s SUMO Protease Animal-Free, His, is
the recombinant SUMO protease fragment from Saccharomyces
cerevisiae. This recombinant enzyme is expressed in E.coli and purified to obtain high yields of the active enzyme. It
is produced under an animal free process, and is suitable for drug and vaccine
development, manufacture and other applications. This product is designed with
a C-terminal 6x His tag which can be removed after the SUMO cleavage reaction
by purification using Ni2+ affinity chromatography resin (Cat. No. L00223) or Ni-charged magnetic beads (Cat. No. L00295).
