500RXN

ABQubit 1X dsDNA HS Assay Kit (Pre-Mixed)

ABQubit dsDNA HS Assay Kit

Entrans 2X qPCR Probe Master Mix is a special reagent for qPCR reactions based on the primer-probe detection. When a probe is bound to the template DNA strand, the 5′-3 ‘exonuclease activity of Taq enzyme can be used to hydrolyze the probe, thereby releasing fluorescent signal. This product uses chemically modified hot-start Taq enzyme for amplification. Accurate quantification, high amplification efficiency, good repeatability and wide credibility. Entrans 2X qPCR Probe Master Mix is a 2X concentration master mix, which contains all the components required for qPCR except primers, probes and templates.

Entrans 2X SNP Genotyping qPCR Probe Master Mix (with UDG)

Entrans qPCR Probe Kit is designed for probe-based qPCR reactions. It contains all components required for a rapid and reliable qPCR reaction except primers, probes and templates. When an intact probe binds to the complementary template DNA strand, the reporter dye and quencher are in proximity and the quencher absorbs the fluorescence emitted by the reporter dye. In PCR extension, the 5 ‘-3 ‘exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. This kit includes an engineered version of Taq polymerase with antibody-mediated Hot-start capability to inhibit non-specific amplification at room temperature, while allowing high efficiency, sensitivity and reproducibility in qPCR amplification. The Entrans qPCR Probe Kit is an ideal product for high-speed multiplex qPCR analyses.

This product utilizes HS Taq DNA Polymerase as the core enzyme and adopts a dual modification method with chemical antibody and modification, aiming to ensure amplification efficiency while significantly enhancing the specificity of the product, suitable for single nucleotide polymorphism (SNP) genotyping detection.

The Fast One Step Probe RT-qPCR Kit is a reagent kit designed for rapid amplification quantitative RT-qPCR detection using the probe method. It includes reaction buffer, hot-start Taq polymerase, novel reverse transcriptase ABScript Ⅲ Reverse Transcriptase, and heat-labile UDG. This kit utilizes RNA as a template and includes gene-specific primers, enabling consecutive reverse transcription and PCR reactions to take place in the same tube without the requirement for additional pipetting or tube opening steps. This streamlined process significantly enhances throughput. The kit utilizes dUTP/UDG to prevent contamination, with the heat-sensitive UDG rapidly degrading uracil-containing contaminants at room temperature. At 50°C during reverse transcription, the heat-labile UDG quickly deactivates, ensuring it does not affect the efficiency and sensitivity of RT-qPCR.

The Lyophilization One-Step RT-qPCR Probe Kit is a universal lyophilized reagent designed for RT-qPCR reactions using probes. It is suitable for lyophilization of freeze-dried powder and freeze-dried microspheres. After freeze-drying into microspheres. This kit includes reaction buffer, hot-start Taq polymerase, novel reverse transcriptase ABScript Ⅲ Reverse Transcriptase, heat-labile UDG, and RNase inhibitor (RI). This kit utilizes RNA as a template and employs gene-specific primers. Reverse transcription and PCR reactions can be performed consecutively in the same tube, eliminating the need for additional tube opening or pipetting steps, significantly enhancing detection throughput. The kit utilizes dUTP/UDG for contamination prevention, with the heat-labile UDG quickly degrading U-containing contaminants at room temperature. The heat-labile UDG is rapidly deactivated at 50°C during reverse transcription, ensuring it does not affect the efficiency and sensitivity of RT-qPCR. This product contains glycerol content below 0.2% and includes lyoprotectants and excipients. It can be stored at 45°C for 6 months without degradation in performance.

Mouse Tissue Direct PCR Kit is specially designed for the rapid genotyping of mouse, which contains Tissue Lysis Buffer II and Powerpol HS 2X PCR Mix for Mouse Genotyping for DNA release and PCR amplification. This kit can be used for the rapid extraction of genomic DNA from mouse tails, ears, toes, and other tissues. The extracted genomic DNA can be used directly as template for PCR amplification, which greatly shortens the experimental time. Tissue Lysis Buffer II in this kit already contains proteinase K, which does not require additional preparation.
Powerpol HS 2X PCR Mix for Mouse Genotyping in this kit only needs to add primers and templates to perform amplification, and the reagent contains loading buffer, PCR products can be directly loaded for electrophoresis.