5mg

2-(Phosphonomethyl)-pentanedioic Acid

4-beta-Phorbol 12-Myristate 13-Acetate

9-cis-Retinoic Acid

Chymostatin

GenCRISPR™ Ultra eSpCas9-2NLS-GMP is utilized for CRISPR gene editing applications. The Cas9 nuclease forms a stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component. With the help of two nuclear localization signals (NLS) expressed with the Cas9 nuclease, the RNP complex enters the nucleus and cleaves target gene. When compared with a plasmid-based delivery system, the RNP delivery system has been observed to increase the on-target gene editing efficiency and decrease off-target effects.

The GenCRISPR™
Ultra eSpCas9 product line provides customers with a selection of research use,
basic GMP and GMP compliant Cas9 nucleases. The Cas9 protein can be formed with
the guide RNA into a ribonucleoprotien (RNP) complex. The use of an RNP complex
to perform gene editing has been shown to reduce the challenges encountered
with other CRISPR gene editing techniques such as viral and plasmid delivery.
Challenges include off-target effects, cell viability and transcription/translational
challenges.

GenCRISPR™
Ultra eSpCas9-2NLS is a mutant form of Cas9 nuclease and is produced by
expression in an E. coli strain
carrying a plasmid encoding the eSpCas9 gene from Streptococcus pyogenes with double-ended nuclear localization
signal (NLS). GenCRISPR™ Ultra eSpCas9-2NLS delivers higher fidelity and less
off-target activity than wild-type SpCas9 nuclease.

The GenCRISPR™
Ultra eSpCas9 product line provides customers with a selection of research use,
basic GMP and GMP compliant Cas9 nucleases. The Cas9 protein can be formed with
the guide RNA into a ribonucleoprotien (RNP) complex. The use of an RNP complex
to perform gene editing has been shown to reduce the challenges encountered
with other CRISPR gene editing techniques such as viral and plasmid delivery.
Challenges include off-target effects, cell viability and transcription/translational
challenges.

GenCRISPR™ Ultra eSpCas9-N-NLS
Research, tag-free is a mutant form of Cas9
nuclease and is produced by expression in an E. coli strain carrying a plasmid encoding the eSpCas9 gene from Streptococcus pyogenes with an N-terminal
nuclear localization signal (N-NLS). GenCRISPR™ Ultra eSpCas9-N-NLS Research,
tag-free delivers higher fidelity
and less off-target activity than wild-type SpCas9 nuclease.

GenCRISPR™ Ultra NLS-Cas9-GMP is utilized for CRISPR gene editing applications. It is manufactured in compliance with ISO 13485 and GMP quality management system standards and with stringent process controls and complete documentation records. This product is a ideal choice for most CRISPR gene editing applications where high editing efficiency is preferred.

The
GenCRISPR™ Ultra Cas9 product line provides customers with a selection of
research use, GMP preclinical and GMP compliant Cas9 nucleases. The Cas9
protein can be formed with the guide RNA into a ribonucleoprotien (RNP)
complex. The use of an RNP complex to perform gene editing has been shown to
reduce the challenges encountered with other CRISPR gene editing techniques
such as viral and plasmid delivery. Challenges include off-target effects, cell
viability and trascprition/translational challenges.

GenCRISPR™ Ultra Cas9 is
produced by expression in an E. coli
strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes with an N-terminal nuclear localization
signal (NLS).

Leptin is a cytokine belonging to the Interleukin 6 family, and has a four-helix bundle structure. Leptin is encoded by the ob gene, and produced and secreted by white adipose tissue. The receptors of Leptin are Type I cytokine receptors, which exist in two different forms: a short form expressed in multiple tissues, and a long form expressed exclusively in the central nervous system (CNS). Upon binding to Leptin, the receptors activate the JAK/STAT3 pathway and PI3K, and stimulate transcriptional programs that regulate feeding behavior, metabolic rate, endocrine axes, and glucose fluxes. The deficiency of Leptin in human and mouse causes morbid obesity, diabetes, and neuroendocrine anomalies. Leptin also has effects on reproduction and immunity. In summary, Leptin is a pivotal cytokine controlling energy balance, and as such has profound effects on human health.

Leptin is a cytokine belonging to the Interleukin 6 family, and has a four-helix bundle structure. Leptin is encoded by the ob gene, and produced and secreted by white adipose tissue. The receptors of Leptin are Type I cytokine receptors, which exist in two different forms: a short form expressed in multiple tissues, and a long form expressed exclusively in the central nervous system (CNS). Upon binding to Leptin, the receptors activate the JAK/STAT3 pathway and PI3K, and stimulate transcriptional programs that regulate feeding behavior, metabolic rate, endocrine axes, and glucose fluxes. The deficiency of Leptin in human and mouse causes morbid obesity, diabetes, and neuroendocrine anomalies. Leptin also has effects on reproduction and immunity. In summary, Leptin is a pivotal cytokine controlling energy balance, and as such has profound effects on human health.

Leupeptin Hemisulfate, Synthetic

Minoxidil Sulfate

Prostaglandin E2

Protein G, a cell surface protein of group G streptococci, is a Type III Fc receptor that binds to the Fc region of IgG by a non-immune mechanism similar to that of protein A from Staphylococcus aureus. This product contains three IgG binding domains, but its albumin-binding region has been removed. Additionally, His-tag and cysteine have been added to N- and C-termini, respectively, to facilitate purification and conjugation. This does not affect the protein’s ability to bind to IgG.

PSA monoclonal antibodies (5B10D4, 8A9B8, 1G9G8, 4A5D10 and 1A7D4) recognize total PSA, freePSA and ACT- PSA Complex (Prostate Specific Antigen / a-1 Antichymotrypsin Complex).

S-Acetyl Coenzyme A Lithium Salt

Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii. Streptavidin can bind to four moles of biotin per mole of protein,and it shows extremely high affinity, approximately 10,000-15,000 Da. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point of 6.5 to avidin’s 10 far closer to that at which most useful biological interactions occur. As a result, Streptavidin frequently exhibits much lower non-specific binding than avidin does.

Streptavidin (Recombinant)

GenScript Streptavidin Antibody is specific to streptavidin.

Tyrphostin AG 1295

Vinpocetine