5ml Resin (Total 10 ml)

GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) features high binding capacity, specificity, sensitivity, stability, and reusability, making it particularly useful for the affinity purification and immunoprecipitation of DYKDDDDK-tagged fusion proteins expressed in common protein expression systems such as bacteria, yeast, and mammalian cells.

GenScript Anti‐DYKDDDDK G1 Affinity Resin (Cat. No. L00432) is designed for the purification of DYKDDDDK‐tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells.

This product is designed for purification of proteins, antibodies and compounds containing (GnS)m linker (n≥2, m≥2) from commonly used protein expression systems such as bacteria, yeast and mammalian cells.

Principle
Add the sample containing the HA-tagged protein
to the Anti-HA IP Resin and allow the protein bind to the Resin during a short
time incubation. Then the HA-tagged protein can be eluted off the beads, or
used directly for pull-down of target protein.

Material Supplied
Genscript Anti-HA IP Resin is an agarose beads
coupled to THE™ HA Tag Antibody, mAb, Mouse (Genscript, A01244).
The antibody recognizes C-terminal, N-terminal, and internal HA tagged-fusion
proteins. It is supplied as a suspension in 50% glycerol containing 10 mM sodium phosphate, 150 mM
sodium chloride, pH 7.4, 0.02 % (w/v) sodium azide (PBS/A).

Storage and Stability
Store the resin: Anti-HA IP Resin is supplied at
-20°C.Do not store the gel at freezing temperatures in the absence of glycerol. 
Store the Column: Wash the column three times
with 5 ml of TBS/A (TBS containing 0.02 % sodium azide) then add another 5 ml
of TBS/A and store at 2-8°C without draining.

Additional Buffers Needed
Equilibration buffer:  50 mM Tris, 150 mM NaCl, pH 7.4.
1×SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at
25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue.

GenScript Anti-His Affinity Resin is designed for purification of His-tagged protein. It is composed of agarose and conjugated THE^TM anti-His monoclonal antibody. His-tagged protein in the crude samples can bind to the anti-His monoclonal antibodies coupled with the agarose, and the nonspecific proteins can be eliminated by washing. As a result, the His-tagged protein will finally be eluted with high recovery. Moreover, this product can be used for immunoprecipitation of His-tagged protein.

Camelid
VHH Antibody (also called single-domain antibody, sdAb or Nanobody by Ablynx)
is a peptide chain about 110 amino acids in length, containing one variable domain
of a heavy-chain antibody (a VHH fragment). Like a whole antibody, the camelid
VHH antibody is able to bind selectively to a specific antigen. This single-domain
antibody has a broad range of applications in biotechnical and therapeutic research
fields due to its small size, simple production and high affinity.
GenScript’s MonoRab™ Anti-Camelid VHH
Affinity Resin (Cat. No. L00905) is designed for purification of Camelid VHH
single domain antibody from commonly used protein expression systems such as bacteria,
yeast and mammalian cells. The VHH single domain antibody in cell lysate can specifically
bind to the Anti-Camelid VHH monoclonal antibody coupled to the resin. Nonspecific
binding reactions are eliminated by stringent washing steps, and the purified Camelid
VHH single domain antibody is eluted with a high recovery rate. Table
1 lists the main characteristics of GenScript’s MonoRab™ Anti-Camelid VHH
Affinity Resin.

 Table 1. Characteristics of Anti-Camelid VHH Affinity
Resin

GenScript’s MonoRabᵀᴹ Anti-DYKDDDDK Affinity Resin is designed for optimal purification of DYKDDDDK-tagged proteins. DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification. Based on our new rabbit monoclonal antibody technology (MonoRabᵀᴹ), this resin offers high binding capacity, high purity, and resistance to high concentrations of salt or acid during protein purification. The binding capacity of MonoRabᵀᴹ Anti-DYKDDDDK Affinity Resin is 1.5 mg DYKDDDDK-tagged protein per mL of settled resin. The recombinant proteins expressed in bacterial or mammalian cells cultures specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin, resulting in highly pure eluates. The resin can withstand stringent washing steps, especially with buffers containing high concentration of salt. The resin is resistant to up to 2 M NaCl, and to buffers with pH as low as 2.5.

GenScript Ni Resin FF is a 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni²⁺ by three coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. Ni Resin FF has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. Ni Resin FF is an excellent choice for high performance purification of polyhistidine-tagged proteins.

GenScript Protein A Resin is an affinity
chromatography medium designed for easy, one-step purification of classes, subclasses
and fragments of immunoglobulins from biological fluids and from cell culture
media. Protein A Resin can also be used for immunoprecipitation of
proteins, protein complexes or antigens. The recombinant protein A ligand is
coupled to 4%
agarose. The coupling is optimized to give high binding capacity for
immunoglobulins. The static binding
capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin.
The binding capacity will
vary depending on several factors such as target antibody, flow rate etc. Table
1 lists the characteristics of Protein A Resin.

Protein A, a bacterial cell wall protein isolated
from Staphylococcus
aureus,
binds to mammalian IgGs mainly through Fc regions. Native
protein A has five IgG binding domains and many other domains with unknown
functions. Recombinant protein A contains five high
affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region
is involved in binding, the Fab region is available for binding antigens.

GenScript’s Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens.The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg rabbit IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc. Table 1 lists the characteristics of Protein A Resin FF.

Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens.

Description:

GenScript Protein G Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin is over 20 mg sheep IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.

Total Volume:

5 ml settled resin (10 ml 50% slurry)

GenScript Protein G Resin FF is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin FF is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Table 1 lists the characteristics of Protein G Resin FF.
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.

Storage

Characteristics

GenScript Streptavidin Resin is an affinity chromatography medium designed for easy,
one-step purification of biotinylated peptides, antibodies, lectins, etc
from samples. The purified recombinant streptavidin is covalently coupling to 4%
highly cross-linked agarose. The
coupling is optimized to give high binding capacity for biotinylated molecules. The total
binding capacity of Streptavidin Resin is more
than 120 nmol of D-Biotin/ml
settled resin. Table 1 lists the characteristics of Streptavidin Resin.

Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces
avidinii. Streptavidin binds 4 moles of biotin per mole of protein with an
extremely high affinity. Streptavidin lacks carbohydrate side chains present on
avidin and has an isoelectric point of 6.5 (vs 10 for avidin) close to
where most useful biological interactions occur. As a result,
streptavidin frequently exhibits much lower non-specific binding than avidin
does.