96RXN

FFPE refers to formalin-fixed paraffin-embedded samples, in which DNA is severely damaged. Damaged DNA Repair Enzymes II is an optimized enzyme mixture primarily used to repair FFPE sample DNA, thereby obtaining high-quality sequencing data.

Dual DNA Adapter 96 Kit for One-step DNA Lib Prep

Fast RNA-seq Lib Prep Kit V2

ABclonal First Strand Synthesis Module (RK20353) is designed for RNA fragmentation and first strand cDNA synthesis in RNA-seq library construction. The second strand cDNA can be directly synthesized without purifying the first strand cDNA product. The RT Reagent in this module does not contain Actinomycin D and can be used with ABclonal Second Strand Synthesis Module (RK20346) to make RNA libraries. In addition, Frag/Elute Buffer employs Mg²⁺ at high temperature conditions to fragment the RNA. Therefore, the RNA sample cannot contain metal ions such as Mg²⁺ or a metal ion chelating agent such as EDTA, which may affect the fragmentation size of RNA.

First Strand Synthesis Module (Stranded)

FS Module

The FS Pro DNA Lib Prep Kit V2 is designed for DNA library preparation for next generation sequencing (NGS) on Illumina® platforms. This kit integrates DNA fragmentation with end repair and dA tailing into one step, and it can be directly followed by adapter ligation without purification. With greatly simplified experimental flow, the entire experiment can be completed within 2 hours. This kit is compatible with DNA samples from different species and different origins. Optimization of reagents and protocol greatly reduced rates of false positives, significantly improving sequencing yield and overall results, including with FFPE DNA samples. This kit is suitable for the construction of PCR-Free libraries (e.g., 100 ng of high-quality genomic DNA).
The components of the kit have undergone strict quality control, mainly including component pollution test, functional test verification, application scenario test and product batch stability test, etc., to minimize the residue of pathogenic bacteria and apply to the detection of pathogenic bacteria.

The FS Pro DNA Lib Prep Kit for Illumina is designed for DNA library preparation for next generation sequencing (NGS) on Illumina® platforms. This kit integrates DNA fragmentation with end repair and dA tailing into one step, and it can be directly followed by adapter ligation without purification. With greatly simplified experimental flow, the entire experiment can be completed within 2 hours. This kit is compatible with DNA samples from different species and different origins. Optimization of reagents and protocol greatly reduced rates of false positives, significantly improving sequencing yield and overall results, including with FFPE DNA samples. This kit is suitable for the construction of PCR-Free libraries (e.g., 100 ng of high-quality genomic DNA).
The components of the kit have undergone strict quality control, mainly including component pollution test, functional test verification, application scenario test and product batch stability test, etc., to minimize the residue of pathogenic bacteria and apply to the detection of pathogenic bacteria.

Gloria Nova HS DNA polymerase is a typical B-family polymerase with a unique structure. It is a new enzyme similar to Pyrococcus furiosus, fused with a continuous synthesis enhancement domain, which improves elongation, yield and specificity.
Gloria Nova HS DNA polymerase is equipped with specific antibodies, which effectively inhibits non-specific amplification and improves the stability of Gloria Nova HS DNA polymerase.
Gloria Nova HS DNA polymerase has 5′-3′ continuous synthesis activity and 3′-5′ exonuclease activity, and its amplified products are blunt-ended.
Gloria Nova DNA polymerase is a mutant polymerase modified by genetic engineering. It has the ability to incorporate dUTP and can also amplify complex templates with dU.
Gloria Nova HS 2X PCR Mix for NGS is an easy-to-operate 2X Mix, which contains Gloria Nova HS DNA polymerase, buffer, Mg⁺ and dNTPs, etc. Only additional DNA template, primers and water are needed in the amplification system. Perform amplification.
All batches of Gloria Nova HS 2X PCR Mix for NGS have undergone strict quality control to ensure the stable performance of each batch of reagents.

The mRNA-seq Lib Prep Module for Illumina® kits provides a method for generating non-directional (non-strand-specific) RNA library that are compatible with the Illumina sequencing platform. The kit contains all of the enzymes and buffers for the First Strand Synthesis Module, Second Synthesis Module, and DNA Lib Prep Module. Its Compatible with Poly(A) mRNA Purification Module（RK20341）, rRNA Depletion module (H/M/R)（RK20348）and multiplexing adaptors and primers. The kit is applicable to animal, plant, and eukaryote (e.g., fungi) total RNA templates as well as the isolated and purified mRNA templates. The recommended total RNA input is 100 ng-1 μg.

One-step DNA Lib Prep Kit for Illumina V2 (1ng Input DNA)

One-step DNA Lib Prep Kit for Illumina V2 (50 ng Input DNA)

One-step DNA Lib Prep Kit for Illumina V2 (5ng Input DNA)

Poly(A) mRNA Capture Module

The Rapid Plus DNA Lib Prep kit provides a streamlined and efficient method to construction DNA library for next generation sequencing (NGS) at Illumina® platforms. The entire protocol can be completed in 2 hours. Starting from fragmented input-DNA (1 – 1000 ng), the kit contains all the enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, the fragmented DNA is end-repaired and adenylated during the initial step. The kit can be conveniently used for construction of PCR-free library from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in the library amplification.
The kit also provides DNA repair enzymes, which could repair DNA damages such as nicks, gaps, oxidized bases, damaged/blocked 3’ ends, AP sites (apurinic/apyrimidinic sites), and uracil bases. In addition, the DNA repair enzymes significantly increase the NGS library yields from FFPE-derived DNA.

The Rapid Plus DNA Lib Prep kit for Illumina® V2 provides a streamlined and efficient method to construct DNA libraries for next generation sequencing (NGS) on Illumina® sequencing platforms. This kit is an updated version of the original Rapid Plus DNA Lib Prep kit (Cat. Nos. RK20208) that has been further optimized to provide superior DNA damage repair and improve ligation efficiency with low-input DNA. The entire protocol can be completed within 2 hours. Starting from fragmented input-DNA (1 – 1000 ng), the kit contains all of the necessary enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, fragmented DNA is end-repaired and adenylated during one initial step. The kit can be conveniently used for construction of PCR-free libraries beginning with 100 ng of high-quality fragmented genomic DNA, or from 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in library amplification.The kit also provides DNA repair enzymes which repair DNA damage such as nicks, gaps, oxidized bases, damaged/blocked 3’ ends, AP sites (apurinic/apyrimidinic sites), and uracil bases. In addition, the DNA repair enzymes significantly increase NGS library yields from FFPE-derived DNA.

Rapid Plus DNA Lib Prep Kit for MGI V2

Rapid Plus DNA Lib Prep Kit V2 (No DDREs) (Cat. NO RK20271) contains the enzymes and buffers required to convert a broad range of input amounts of DNA into high quality libraries for next-generation sequencing on the Illumina® NGS platforms. Inputs of 1 ng to 1000 ng double-stranded DNA (dsDNA) are required for library preparation. The entire four-step workflow takes place in a single tube or well, in about three hours (Figure 1). No intermediate purification steps or sample transfers are necessary, thus preventing handling errors and loss of valuable samples.
Once purified and quantified, the resulting libraries are ready for Illumina NGS instruments using standard Illumina sequencing reagents and protocols. The kit provides excellent results for high-coverage deep sequencing, such as de novo sequencing, whole genome resequencing, whole exome sequencing, and other enrichment techniques.

rRNA Depletion Kits (Human rRNA & Globin) (ABclonal, Cat. RK30209) is specifically designed for human blood samples to remove rRNA and Globin mRNA from total RNA, suitable for 10-1000 ng total RNA templates.
This kit primarily includes four steps: hybridization of Ribo-Pools probes to target RNA, streptavidin magnetic bead pretreatment, rRNA removal, and RNA purification. The entire procedure can be completed within 60 min without enzymatic digestion reactions and is compatible with high-throughput automation. By utilizing the streptavidin magnetic bead adsorption method, it effectively removes rRNA (including cytoplasmic rRNA and mitochondrial rRNA) and Globin mRNA while preserving other mRNAs and non-coding RNAs for subsequent applications such as whole transcriptome sequencing, long non-coding RNA sequencing, and related research fields.

rRNA Depletion Kits (Pan-Prokaryote) (ABclonal, Cat. RK30207) is an efficient rRNA removal kit specifically designed for prokaryotes, including bacteria and archaea. This kit is suitable for total RNA samples ranging from 10 to 400 ng and effectively removes 23S, 16S, and 5S rRNA from mixed bacterial samples or single strains using streptavidin magnetic beads.
The kit’s workflow consists of four main steps: hybridization of Ribo-Pools probes with target RNA, pre-treatment with streptavidin magnetic beads, rRNA removal, and RNA purification. The entire process can be completed within 60 minutes without the need for enzymatic digestion and is compatible with high-throughput automation. Since it does not rely on poly A selection, this kit is suitable for the analysis of non-polyadenylated RNA, including non-coding RNA, histones, and RNA sequencing analysis of prokaryotes.

rRNA Depletion Kits (Plant) (ABclonal, Cat. RK30208) is a highly efficient rRNA removal kit specifically developed for angiosperms (leaf, seed, and root tissues). This kit is suitable for plant total RNA samples with a template amount of 10-1000 ng, effectively removing cytoplasmic rRNA (including cytoplasmic 5S rRNA, 5.8S rRNA, 18S rRNA, and 28S rRNA), plastid rRNA, and mitochondrial rRNA (including 12S rRNA and 16S rRNA) from total RNA through streptavidin magnetic bead adsorption.
The kit’s workflow consists of four main steps: hybridization of Ribo-Pools probes with target RNA, pre-treatment with streptavidin magnetic beads, rRNA removal, and RNA purification. The entire process can be completed within 60 minutes without the need for enzymatic digestion and is compatible with high-throughput automation. The kit does not rely on poly A selection and can be used for analyzing non-polyadenylated RNA, including non-coding RNA, histones, and whole transcriptome analysis.

The rRNA depletion module (H/M/R) is primarily designed for total RNA samples from humans, mice, and rats. It can effectively remove cytoplasmic rRNA (including cytoplasmic 5S rRNA, 5.8S rRNA, 18S rRNA, and 28S rRNA) and mitochondrial rRNA (including 12S rRNA and 16S rRNA) from total RNA. The resulting RNA can be used for library construction in Whole RNA-seq or other rRNA depletion analyses.
This product is suitable not only for total RNA samples with good integrity but also for degraded RNA samples, such as FFPE RNA. The required starting amount of total RNA is 10 ng to 1 μg.

Scale Methyl-DNA Lib Prep Kit for Illumina

Scale ssDNA-seq Lib Prep Kit for Illumina V2

The ABclonal Second Strand Synthesis Module (RK20346) uses productfrom the ABclonal First Strand cDNA Synthesis Module (RK20342 orRK20353) as a template for second strand cDNA synthesis to generate adouble-stranded cDNA product. This double-stranded cDNA product canbe directly used for DNA library construction.

Second Strand Synthesis Module (Stranded)

Single Cell/Low Input cDNA Synthesis & Amplification Module

The Small RNA-seq Lib Prep Kit for Illumina V3 (ABclonal, Cat. RK20312) is a kit designed for the preparation of small RNA libraries compatible with the Illumina high-throughput sequencing platform. It is suitable for total RNA samples from animals and plants, or purified small RNA samples, with template starting amounts ranging from 100 to 1000 ng. This kit is also compatible with RNA samples derived from sources such as serum, plasma, and exosomes.
Small RNA library preparation procedures include: ligation with the respective universal adapters at the 3′ and 5′ ends of the small RNA, reverse transcription, amplification by PCR, purification with magnetic beads, PAGE gel extraction, etc. The obtained target library is sequenced on the Illumina sequencing platform. For standard miRNA libraries, the fragment size is about 150 bp. The fragment at about 130 bp is the adapter dimer.The adapters provided in the kit are truncated, so they need to be used with the Small RNA Adapter Module for Illumina (RK20358/RK20359/RK20360), and PCR is required to ensure an intact library structure and enrich the library.
All reagents (including all enzymes and buffers) provided in the kit have undergone rigorous quality control and have been verified through library preparation and sequencing, ensuring stability and repeatability.

Stranded mRNA-seq Lib Prep Module for Illumina

TruePol 2X PCR Mix for NGS is a premixed solution derived from polymerase of the B family. It is easy to use, containing DNA polymerase, buffer, Mg2+, and dNTPs. For amplification, simply add DNA template, primers, and water to the system for amplification. This polymerase comes from a unique polymerase mutation library, offering strong extension capabilities that improve yield and specificity without significant GC bias. It has 5′-3′ continuous synthesis activity and 3′-5′ exonuclease activity, resulting in blunt-end amplification products.
All batches of TruePol Mix undergo rigorous quality control to ensure the consistent performance of each batch of reagent.