Description
Accuris 1st Strand cDNA Synthesis Flex Kit includes four components: a high capacity Reverse Transcriptase, an optimized 5X buffer, and separate solutions of oligo (dT) primers, and random hexamer primers. This multi-component format is ideal for 10pg to 2.0µg of input RNA and allows for greater flexibility in assay design. High quality, PCR ready cDNA is quickly generated with the Accuris qMax First Strand cDNA Synthesis Flex Kit. The exceptionally thermostable MMLV- derived qMax High Capacity Reverse Transcriptase combined with a potent RNase inhibitor allows for transcription to take place at higher temperatures (alleviating secondary structure problems) and maintains the integrity of the total RNA. The 5X buffer provides the perfect environment for highly efficient cDNA synthesis and greater yields. The buffer is optimized for superior data accuracy and reproducibility with a wide range of RNA sources.
• Separate solutions of oligo (dT) primers and random hexamer primers for greater flexibility in assay design.
• Input range of 10pg to 2g of total RNA
• Thermostable, high capacity reverse transcriptase and optimized next generation buffer system provide consistent, high yields of fragments up to 9Kb in length
• Cat. No. 735-10074: Accuris qMax First Strand cDNA Synthesis Flex Kit, sample pack, 5 reactions: Includes 10ul RT, 40l 5X Buffer, 20l Random
Hexaprimers and 20l Oligo (dT) Primers
• Cat. No. 735-10077: Accuris qMax First Strand cDNA Synthesis Flex Kit, 50 reactions: Includes 50l of RT, 200l 5X buffer, 100l Random Hexaprimers and 100l Oligo (dT) Primers
• Cat. No. 735-10080: Accuris qMax First Strand cDNA Synthesis Flex Kit, 200 reactions: Contains 4×50 reaction packs (see above)
†Upon receipt, immediately store at -20°C. Avoid excessive freeze/thaw cycles. When stored as directed, this product will retain its activity for 12 months from date of receipt.
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