Description
Product is the lyophilized powder of goat antiserum to human alpha-1-antitrypsin and buffer salts. The antiserum is prepared by hyperimmunizing goats with highly purified human alpha-1-antitrypsin. Antiserum is delipidated and made specific by solid-phase absorption. The resulting antiserum is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
USES: This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques.
SUGGESTED WORKING DILUTIONS (It is recommended that each lab obtain their own optimal working dilution for each assay):
1:50 – 1:200 for Tissue Staining
1:100 – 1:500 for Immunoblotting
1:400 – 2,000 for ELISA
RECONSTITUTION: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.