Description
This Tmem119 antibody has been knockout validated in IHC, meaning it demonstrated the expected staining in wild type mouse brain sections and no staining was observed in Tmem119 knockout mouse brain sections. This data is shown on this datasheet in the images section. To detect mouse Tmem119 by flow cytometry, we recommend using ab210405. To detect human TMEM119 by IHC, we recommend using ab185333.
IHC-Frozen protocol advice:
For immunohistochemistry on frozen sections, it is recommended that a high concentration of Triton X-100 (0.5%) is used during permeabilization and antibody incubation steps. This may increase the proportion of microglia that stain positive for Tmem119.