DMEM/F-12, HEPES

Description

• • Contains four times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium

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• • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate

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• • Contains no proteins, lipids, or growth factors

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• • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)

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• • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO₂ environment to maintain physiological pH

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• • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

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