DNA Polymerase I, Large (Klenow) Fragment

$33.81

DNA Polymerase I, Large (Klenow) Fragment (about 68 kD) is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3’→5′ exonuclease activity, but has lost 5’→3′ exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5′ termini.
It is applicable to DNA sequencing by the Sanger dideoxy method, fill-in of 5′ overhangs to form blunt ends, removal of 3′ overhangs to form blunt ends, second strand cDNA synthesis and second strand synthesis in mutagenesis protocols.

Weight 1.00 lbs
Dimensions 5.00 × 5.00 × 5.00 in
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SKU: Abclonal-RK20525-200U
SKU: Abclonal-RK20525-200U Category: Brand:

Description

Size quantity : 1-EA
Description : DNA Polymerase I, Large (Klenow) Fragment (about 68 kD) is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3’→5′ exonuclease activity, but has lost 5’→3′ exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5′ termini.
It is applicable to DNA sequencing by the Sanger dideoxy method, fill-in of 5′ overhangs to form blunt ends, removal of 3′ overhangs to form blunt ends, second strand cDNA synthesis and second strand synthesis in mutagenesis protocols.

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