Description
Size quantity : 1-EA
Description : DNA Polymerase I, Large (Klenow) Fragment (about 68 kD) is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3’→5′ exonuclease activity, but has lost 5’→3′ exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5′ termini.
It is applicable to DNA sequencing by the Sanger dideoxy method, fill-in of 5′ overhangs to form blunt ends, removal of 3′ overhangs to form blunt ends, second strand cDNA synthesis and second strand synthesis in mutagenesis protocols.


