Description
DWK Life Sciences (Wheaton) Tissue Grinders enable primary cells to be prepared from excised tissues, as well as subcellular components from harvested cell packs. Tissue grinders are used to extract DNA, RNA and proteins for blots or other procedures. Tissue grinders are also used to enhance recovery of other cell products. Capacities on the grinders range from 0.1 mL to 55 mL. There are a variety of grinders to suit many tissue types. Tapered tissue grinders are ideal for connective tissue including heart, muscle and lung. These glass-on-glass grinders feature a long taper design that maximizes grinding efficiency. The Potter-Elvehjem grinder incorporates a serrated pestle design to disperse homogenate into the mortar cylinder. Standard units with PTFE pestles are well suited for soft tissue, such as brain and liver. Tenbroeck grinders have a hollow handle that allows packing with ice, making them suitable for tissue such as liver, intestines and heart. The Dounce Grinder offers two pestles: a loose-fitting pestle for sample reduction and a second, tighter pestle for complete homogenization. The ball-shaped pestles leave cell nuclei intact in soft tissues.
These tapered tissue grinders have an improved design, which incorporates a longer tapered surface on both the mortar and pestle. The conical surface allows initial size reduction followed by passage through the cylindrical section for the final homogenization step. Grinding efficiency is improved, and less time is required as compared to the Tenbroeck and Potter-Elvehjem designs. When the pestle is bottomed within the mortar, the clearance is a uniform .004 to .006 (0.1 to 0.15 mm). The components are interchangeably ground, which allows easier replacement of parts; there is also no need to keep parts matched during cleaning. The ground glass surface on both the mortar and pestle are capable of homogenizing connective tissue including heart, muscle and lung. This unit can also be used for homogenizing skin and plant tissue, and is made from 33 low extractable borosilicate glass (meets ASTM Type I Class A and USP Type I Standards).
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