Description
Enterokinase (EK) is a serine protease produced
by cells of the duodenum, and is involved in mammalian digestion. It plays the role
of turning trypsinogen to the active form of trypsin, and indirectly activates
the pancreatic digestive enzymes. Enterokinase is a specific protease that
cleaves after a lysine at its cleavage site: Asp-Asp-Asp-Asp-Lys. Enterokinase does
not function if the cleavage site is followed by a proline.
GenScript’s Enterokinase Animal-Free, His,
Bovine is the light chain catalytic subunit of bovine enterokinase consisting
of a single glycosylated polypeptide chain containing Ile801-His1035 (Accession
# P98072). This recombinant protein is expressed in P. pastoris and purified to obtain high yields of the active
enzyme. It is produced under an animal free process, and is suitable for drug and
vaccine development, manufacture and other applications. This product is
designed with a C-terminal 6 × His tag and can be removed after cleavage by Ni2+ affinity
chromatography in resin (Cat. No. L00223) or magnetic
beads (Cat. No. L00295).