Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC “Isomer I”)-conjugated goat F(ab’)2 fragment to guinea pig IgG (whole molecule) and buffer salts. Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.

USES

This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab’)2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

SPECIFICATIONS

The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The F(ab’)2 fragments are tested for purity and specificity at a minimum of 20 mg/ml using immunoelectrophoresis. The product is goat F(ab’)2; no trace of albumin, whole molecule IgG or Fc is detected. This product shows reactivity to guinea pig IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG, some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.

RECONSTITUTION

Reconstitute product with 1.0 ml of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.

STORAGE

Store lyophilized product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage, aliquot and freeze reconstituted product at -20°C or lower.