Description
The Fragile X Syndrome is identified by an ampiflication of the FMR1 (CGG)n repeat region. The Fragile X CHEMITM probe is a digoxigenin labeled DNA probe (pFxa1NHE) which can be used in conjunction with the Sure Blot® CHEMITM Kit (Cat. No. 11S4250KIT) to detect (CGG) repeats in the FMR-1 gene. The same results are obtained as when using the standard P32-labeled probes, but without radioactivity. The amplification of the FMR-1 gene is detected on Southern blots by an increase in the band sizes. Also provided is a ready-to-use mixture of unlabeled and digoxigenin-labeled Lambda DNA/Hind III Molecular Weight Markers for convenient visualization on both ethidium bromide-stained agarose gels and Southern blots.