Description
-
- HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media
n
-
- The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator
n
-
- A good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable
n
-
- It also has very low visible and UV light absorbance
n
-
- Store at 2°C to 8°C
n