Description
Glycogen Assay Kit ab65620 is an easy and accurate assay to measure glycogen levels in biological samples. In the glycogen assay protocol, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a product that reacts with OxiRed probe to generate color (570 nm) and fluorescence (Ex 535/Em 587). The assay can detect glycogen 0.04 to 2 mg/ml.
Glycogen assay protocol summary:
– add samples and standards to wells
– add hydrolysis enzyme mix and incubate for 30 min
– add reaction mix and incubate for 30 min
– analyze with microplate reader
If your sample is likely to contain reducing substances, we recommend using Glycogen Assay Kit II (ab169558), as reducing substances may interfere with the assay detection method.