HisPur™ Ni-NTA Resin

Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin.nn nnThe specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.nnHisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.nnHighlights:n

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  • High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin

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• • Versatile – purify proteins using native or denaturing conditions

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• • Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives

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• • Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges

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• • Cost-effective – reuse the same batch of resin at least five times

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• • Easy to use – pre-formulated buffers available for kit formats

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nAvailable Formats:n

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  • Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles

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• • Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns

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• • Purification Kits – complete kits in all three column sizes

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• • Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing

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• • Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin

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